Microbacterium phage Knox

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ilzatvirus; unclassified Ilzatvirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0HMN3|A0A2L0HMN3_9CAUD Tail assembly chaperone OS=Microbacterium phage Knox OX=2079586 GN=16 PE=4 SV=1
MM1 pKa = 7.49IQMACTDD8 pKa = 4.49CDD10 pKa = 4.26DD11 pKa = 4.15KK12 pKa = 11.46QQEE15 pKa = 4.4PFSEE19 pKa = 4.6TEE21 pKa = 3.94GEE23 pKa = 4.5GTVDD27 pKa = 3.4TQEE30 pKa = 4.49TPSEE34 pKa = 4.49VYY36 pKa = 10.59DD37 pKa = 3.71HH38 pKa = 7.36AEE40 pKa = 3.76HH41 pKa = 6.96FGPPEE46 pKa = 3.55AA47 pKa = 5.69

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0HMT3|A0A2L0HMT3_9CAUD Uncharacterized protein OS=Microbacterium phage Knox OX=2079586 GN=49 PE=4 SV=1
MM1 pKa = 7.2TVATYY6 pKa = 10.18IIARR10 pKa = 11.84TGKK13 pKa = 9.94GRR15 pKa = 11.84PTLQHH20 pKa = 6.76ALADD24 pKa = 4.25DD25 pKa = 4.41GQRR28 pKa = 11.84TVCGLDD34 pKa = 3.0VSYY37 pKa = 10.49WSRR40 pKa = 11.84AYY42 pKa = 9.87QSRR45 pKa = 11.84PITEE49 pKa = 3.92IVCRR53 pKa = 11.84KK54 pKa = 7.89CAKK57 pKa = 10.17RR58 pKa = 11.84LTGSAA63 pKa = 3.72

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13221

47

808

209.9

23.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.735 ± 0.45

0.658 ± 0.098

6.429 ± 0.271

6.52 ± 0.45

3.063 ± 0.202

7.874 ± 0.362

1.876 ± 0.191

5.06 ± 0.393

4.69 ± 0.375

8.532 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.155

3.207 ± 0.167

4.924 ± 0.235

4.069 ± 0.189

5.741 ± 0.339

6.021 ± 0.238

6.414 ± 0.294

7.443 ± 0.29

2.133 ± 0.228

3.094 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski