Acetobacter pasteurianus (strain NBRC 3283 / LMG 1513 / CCTM 1153)
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2906 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7JH16|C7JH16_ACEP3 Phosphoribosyl-ATP pyrophosphatase OS=Acetobacter pasteurianus (strain NBRC 3283 / LMG 1513 / CCTM 1153) OX=634452 GN=hisE PE=3 SV=1
MM1 pKa = 7.87 PIITVVGASGNIQVTVDD18 pKa = 3.73 GAQNSALYY26 pKa = 10.04 NQATDD31 pKa = 4.36 LSNQLSSVISTLDD44 pKa = 3.38 AQNLSAGDD52 pKa = 3.71 TTFSDD57 pKa = 4.07 SNKK60 pKa = 10.12 AGYY63 pKa = 10.04 GVITSAGSYY72 pKa = 9.18 RR73 pKa = 11.84 VAGNVEE79 pKa = 3.96 YY80 pKa = 11.0 LGIGSDD86 pKa = 3.8 ARR88 pKa = 11.84 SQPLIALNGQVTVDD102 pKa = 3.48 AVGVTSKK109 pKa = 11.26 NMTILGGTNTGIAFYY124 pKa = 10.44 AGSQSGQFLAGAGANLFEE142 pKa = 5.68 GNSQYY147 pKa = 11.37 DD148 pKa = 3.29 AGNWSIMTGNGNDD161 pKa = 3.78 TVNSGAGNNTISAGLGHH178 pKa = 5.91 NTIDD182 pKa = 4.28 LGSGMNYY189 pKa = 8.49 VHH191 pKa = 7.38 SDD193 pKa = 3.33 GQDD196 pKa = 3.18 TITATSGRR204 pKa = 11.84 QSVTLSGSSSTVQLSDD220 pKa = 2.93 NSLVVDD226 pKa = 4.91 ANSSQQITVGGASTVTGGSLDD247 pKa = 3.89 YY248 pKa = 11.07 INFSGATGTVEE259 pKa = 4.47 GGQNSTISAAHH270 pKa = 6.63 GNLQTEE276 pKa = 4.54 NTDD279 pKa = 3.24 SALINVSDD287 pKa = 3.92 NLTFIGGTGEE297 pKa = 4.08 TTITAGHH304 pKa = 5.78 ATIFGSNGLDD314 pKa = 2.93 IHH316 pKa = 7.11 VSASQQGTIDD326 pKa = 3.46 GAGANNLFVANDD338 pKa = 3.66 GNEE341 pKa = 4.25 TLDD344 pKa = 3.89 GASSAFGFQAFGNNAGTTGTQTFIGGTASDD374 pKa = 3.79 TLVAGVGNATLEE386 pKa = 4.6 GGSGAANVFGFRR398 pKa = 11.84 NSVAGADD405 pKa = 3.68 YY406 pKa = 9.44 TIQDD410 pKa = 4.0 FGSAANNSVLLVDD423 pKa = 3.86 YY424 pKa = 10.2 DD425 pKa = 3.71 YY426 pKa = 11.63 TKK428 pKa = 11.27 ASFQTEE434 pKa = 4.28 VLDD437 pKa = 4.39 KK438 pKa = 10.32 ATHH441 pKa = 6.1 NGGNTTITLSDD452 pKa = 3.42 HH453 pKa = 5.64 SQITFVNVDD462 pKa = 3.93 TLNEE466 pKa = 4.04 NQFSGLKK473 pKa = 9.94
Molecular weight: 47.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.617
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.821
Sillero 3.973
Patrickios 1.214
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|C7JEJ0|C7JEJ0_ACEP3 Uncharacterized protein OS=Acetobacter pasteurianus (strain NBRC 3283 / LMG 1513 / CCTM 1153) OX=634452 GN=APA01_06710 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.03 IIGNRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.41 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2906
0
2906
941337
26
2008
323.9
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.331 ± 0.053
1.135 ± 0.016
5.226 ± 0.035
5.313 ± 0.057
3.64 ± 0.03
8.019 ± 0.05
2.464 ± 0.023
5.072 ± 0.034
3.637 ± 0.035
10.178 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.022
3.062 ± 0.033
5.389 ± 0.041
3.95 ± 0.03
6.453 ± 0.051
5.799 ± 0.035
5.75 ± 0.038
7.138 ± 0.041
1.455 ± 0.019
2.367 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here