Rhodopseudomonas palustris (strain BisB18)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Rhodopseudomonas; Rhodopseudomonas palustris

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q21B60|Q21B60_RHOPB Ribonuclease VapC OS=Rhodopseudomonas palustris (strain BisB18) OX=316056 GN=vapC PE=3 SV=1
MM1 pKa = 7.35SLSGALSSAISALSAQSSAMAMISDD26 pKa = 5.2NIANSQTVGYY36 pKa = 7.61KK37 pKa = 6.88TTSASFQALVTSSSSATTYY56 pKa = 10.99SSGGVTTLSRR66 pKa = 11.84SNISEE71 pKa = 4.0QGLLTTTTSATDD83 pKa = 3.09IGISGNGFFPVTTALTGGSTLYY105 pKa = 10.03TRR107 pKa = 11.84SGSFTIDD114 pKa = 3.08DD115 pKa = 3.5SGYY118 pKa = 10.05LVSSGGAYY126 pKa = 10.69LLGWRR131 pKa = 11.84TDD133 pKa = 3.06ADD135 pKa = 4.03GNITGSADD143 pKa = 3.61SNNLEE148 pKa = 4.73PINTAVAATSGSASTEE164 pKa = 3.92TKK166 pKa = 10.33FALNLPADD174 pKa = 4.19AATDD178 pKa = 3.62ATFTSSMSVYY188 pKa = 10.57DD189 pKa = 3.85SLGTASSIQVTWTKK203 pKa = 10.09TGSNTWSASFGNPTLSSDD221 pKa = 3.51STTSTGTTTGSATITFNEE239 pKa = 4.59DD240 pKa = 2.34GSLASQSATDD250 pKa = 4.53ISVAWSTGAATSTIALNFGTVGGTDD275 pKa = 3.43GLTQKK280 pKa = 11.05ASGEE284 pKa = 4.3TTPSINLTSIEE295 pKa = 4.36SDD297 pKa = 2.8GLAYY301 pKa = 10.38GKK303 pKa = 10.35LSSIAVGDD311 pKa = 3.98DD312 pKa = 3.23GKK314 pKa = 11.91VNATYY319 pKa = 11.33SNGEE323 pKa = 3.86TIAIYY328 pKa = 9.78KK329 pKa = 10.13IPVATFSAPDD339 pKa = 3.33QLVAKK344 pKa = 10.75SNGLYY349 pKa = 10.23AASAASGTAMVQASGTNGAGTIYY372 pKa = 10.55GGEE375 pKa = 4.3LEE377 pKa = 5.44ASTSDD382 pKa = 3.39TNQEE386 pKa = 3.88FSNMISAQQAYY397 pKa = 9.53SAAAQVMSAVSDD409 pKa = 3.65MYY411 pKa = 10.8DD412 pKa = 3.35TLISAMRR419 pKa = 3.98

Molecular weight: 41.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q21B53|Q21B53_RHOPB Pyrimidine 5-nucleotidase OS=Rhodopseudomonas palustris (strain BisB18) OX=316056 GN=RPC_0812 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4864

0

4864

1559608

35

4435

320.6

34.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.081 ± 0.044

0.884 ± 0.012

5.648 ± 0.028

5.198 ± 0.032

3.624 ± 0.02

8.267 ± 0.039

1.982 ± 0.018

5.311 ± 0.026

3.432 ± 0.03

10.187 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.017

2.611 ± 0.022

5.265 ± 0.034

3.382 ± 0.02

7.087 ± 0.038

5.685 ± 0.029

5.235 ± 0.028

7.434 ± 0.027

1.232 ± 0.013

2.134 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski