Isobaculum melis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Isobaculum

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2700 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9S4T9|A0A1H9S4T9_9LACT Trk system potassium uptake protein TrkA OS=Isobaculum melis OX=142588 GN=SAMN04488559_106100 PE=4 SV=1
MM1 pKa = 7.45SHH3 pKa = 6.05EE4 pKa = 4.9HH5 pKa = 6.8DD6 pKa = 4.67HH7 pKa = 6.9DD8 pKa = 4.0HH9 pKa = 7.29HH10 pKa = 7.48DD11 pKa = 3.89HH12 pKa = 5.66EE13 pKa = 6.33HH14 pKa = 5.34ITVVDD19 pKa = 3.84EE20 pKa = 4.5NGNEE24 pKa = 3.83EE25 pKa = 4.35LYY27 pKa = 10.83EE28 pKa = 3.88ILFTFDD34 pKa = 2.91SDD36 pKa = 5.19DD37 pKa = 3.5YY38 pKa = 10.46GKK40 pKa = 10.75SYY42 pKa = 10.87VIVYY46 pKa = 8.04PAGIGEE52 pKa = 4.32GEE54 pKa = 4.22EE55 pKa = 4.27VEE57 pKa = 4.48LQAFSYY63 pKa = 10.74VEE65 pKa = 4.3SEE67 pKa = 4.11EE68 pKa = 4.19GMQGALNPIEE78 pKa = 5.71SNQEE82 pKa = 2.92WDD84 pKa = 3.44MVEE87 pKa = 4.16EE88 pKa = 4.07VLNTFLADD96 pKa = 3.51EE97 pKa = 4.63EE98 pKa = 4.55EE99 pKa = 4.26

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9RJU1|A0A1H9RJU1_9LACT Peptidoglycan hydrolase OS=Isobaculum melis OX=142588 GN=SAMN04488559_10494 PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.12QKK5 pKa = 7.54THH7 pKa = 6.73RR8 pKa = 11.84GSAKK12 pKa = 9.69RR13 pKa = 11.84FKK15 pKa = 10.09RR16 pKa = 11.84TGNGGLKK23 pKa = 9.56RR24 pKa = 11.84HH25 pKa = 5.79SAFTSHH31 pKa = 5.81MFANKK36 pKa = 8.3STKK39 pKa = 8.24QKK41 pKa = 10.61RR42 pKa = 11.84KK43 pKa = 9.11LRR45 pKa = 11.84KK46 pKa = 9.34ASMVSKK52 pKa = 11.05GDD54 pKa = 3.54FKK56 pKa = 11.14RR57 pKa = 11.84IRR59 pKa = 11.84QQLSQMKK66 pKa = 9.9

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2700

0

2700

818632

28

2247

303.2

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.371 ± 0.058

0.625 ± 0.014

5.111 ± 0.033

7.395 ± 0.058

4.541 ± 0.045

6.457 ± 0.047

1.929 ± 0.021

7.863 ± 0.039

7.093 ± 0.042

9.978 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.025

4.501 ± 0.035

3.387 ± 0.028

4.401 ± 0.04

3.316 ± 0.033

5.745 ± 0.038

6.077 ± 0.053

6.785 ± 0.042

0.846 ± 0.015

3.732 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski