Coprococcus eutactus CAG:665
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2415 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5WHH5|R5WHH5_9FIRM Uncharacterized protein OS=Coprococcus eutactus CAG:665 OX=1263071 GN=BN751_01132 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.49 KK3 pKa = 10.03 KK4 pKa = 10.66 GYY6 pKa = 10.5 GKK8 pKa = 10.56 ALTLLLVTSLVAGTVLTGCGKK29 pKa = 10.62 KK30 pKa = 10.15 KK31 pKa = 10.25 VDD33 pKa = 3.55 YY34 pKa = 11.74 NMGDD38 pKa = 3.77 DD39 pKa = 4.1 EE40 pKa = 4.85 NGSGGGGKK48 pKa = 9.41 LASRR52 pKa = 11.84 LDD54 pKa = 3.62 VPDD57 pKa = 3.97 SYY59 pKa = 11.65 EE60 pKa = 4.01 GALEE64 pKa = 5.49 GIDD67 pKa = 3.57 TDD69 pKa = 3.83 ATGLTDD75 pKa = 3.76 VKK77 pKa = 10.84 INASKK82 pKa = 9.04 ITVPDD87 pKa = 3.64 TDD89 pKa = 3.75 KK90 pKa = 11.26 MSILYY95 pKa = 9.77 YY96 pKa = 10.39 NQNQVDD102 pKa = 3.81 NEE104 pKa = 4.21 YY105 pKa = 10.97 KK106 pKa = 10.58 KK107 pKa = 10.57 RR108 pKa = 11.84 VCEE111 pKa = 3.92 NFFDD115 pKa = 4.09 VSAGVYY121 pKa = 8.34 TYY123 pKa = 10.93 SWEE126 pKa = 4.09 KK127 pKa = 9.96 PYY129 pKa = 10.98 KK130 pKa = 10.2 GDD132 pKa = 4.26 LEE134 pKa = 4.72 RR135 pKa = 11.84 EE136 pKa = 3.83 IEE138 pKa = 4.16 NYY140 pKa = 10.4 EE141 pKa = 4.17 EE142 pKa = 4.58 LSKK145 pKa = 11.16 QSTSDD150 pKa = 3.57 DD151 pKa = 3.53 EE152 pKa = 5.5 KK153 pKa = 11.57 SFFDD157 pKa = 5.75 DD158 pKa = 4.35 YY159 pKa = 11.36 ISSLKK164 pKa = 10.2 EE165 pKa = 3.75 QLKK168 pKa = 8.1 TATDD172 pKa = 3.53 EE173 pKa = 4.31 RR174 pKa = 11.84 EE175 pKa = 4.05 GAGDD179 pKa = 3.53 YY180 pKa = 11.05 SADD183 pKa = 3.31 AFVGSKK189 pKa = 10.45 GEE191 pKa = 3.92 NMYY194 pKa = 10.01 MISFNSTEE202 pKa = 4.15 TGEE205 pKa = 4.28 SGGFSIDD212 pKa = 3.98 YY213 pKa = 10.13 YY214 pKa = 10.6 PSDD217 pKa = 3.04 QLINYY222 pKa = 7.47 RR223 pKa = 11.84 PKK225 pKa = 9.79 EE226 pKa = 4.12 GASSVYY232 pKa = 10.19 CYY234 pKa = 10.96 SSDD237 pKa = 3.99 YY238 pKa = 11.33 YY239 pKa = 11.25 DD240 pKa = 5.64 GEE242 pKa = 4.44 DD243 pKa = 3.43 TANTATVSQDD253 pKa = 3.36 DD254 pKa = 5.15 AIQQGLSFLAGCGISDD270 pKa = 4.98 IIEE273 pKa = 4.34 TGCTDD278 pKa = 6.08 LIWEE282 pKa = 4.47 YY283 pKa = 11.56 SDD285 pKa = 3.24 TSYY288 pKa = 9.9 NTLASEE294 pKa = 3.99 KK295 pKa = 9.13 SGYY298 pKa = 10.02 VITYY302 pKa = 8.41 KK303 pKa = 10.6 RR304 pKa = 11.84 SVDD307 pKa = 4.03 GIAPYY312 pKa = 9.41 TPNVYY317 pKa = 10.71 NIDD320 pKa = 4.02 SLNSSDD326 pKa = 4.88 DD327 pKa = 3.22 VWYY330 pKa = 8.94 DD331 pKa = 3.38 TMDD334 pKa = 3.31 EE335 pKa = 4.23 TFEE338 pKa = 5.03 LQIDD342 pKa = 3.9 DD343 pKa = 4.11 NGIVSAYY350 pKa = 10.2 CYY352 pKa = 10.46 DD353 pKa = 3.73 YY354 pKa = 11.37 FKK356 pKa = 11.03 ATGDD360 pKa = 3.27 KK361 pKa = 10.74 KK362 pKa = 11.29 EE363 pKa = 4.0 NVDD366 pKa = 4.42 LISWEE371 pKa = 4.29 DD372 pKa = 3.39 AVKK375 pKa = 10.6 ALPKK379 pKa = 10.42 AVNTYY384 pKa = 8.66 YY385 pKa = 11.1 AEE387 pKa = 4.4 NKK389 pKa = 7.57 TQYY392 pKa = 11.33 SSIEE396 pKa = 4.0 FNNVQLAYY404 pKa = 10.81 YY405 pKa = 9.49 KK406 pKa = 10.54 IKK408 pKa = 11.11 DD409 pKa = 3.42 GDD411 pKa = 3.61 KK412 pKa = 10.96 YY413 pKa = 11.19 EE414 pKa = 4.3 YY415 pKa = 10.87 LPVWAFAQCEE425 pKa = 4.2 KK426 pKa = 10.49 TGDD429 pKa = 3.81 GDD431 pKa = 4.13 SSQSEE436 pKa = 4.45 DD437 pKa = 3.71 GLDD440 pKa = 3.42 VSNPSQLIMLNAEE453 pKa = 3.97 TGEE456 pKa = 4.44 LIDD459 pKa = 5.36 LKK461 pKa = 11.12 SVLNTQSFSYY471 pKa = 10.46 TDD473 pKa = 3.44 STVVGGDD480 pKa = 3.79 DD481 pKa = 5.46 DD482 pKa = 5.73 NVTLDD487 pKa = 5.61 DD488 pKa = 4.8 SDD490 pKa = 5.1 LDD492 pKa = 3.85 EE493 pKa = 6.55 DD494 pKa = 4.44 GASIADD500 pKa = 3.97 DD501 pKa = 3.78 SAVSDD506 pKa = 4.54 DD507 pKa = 5.01 SSDD510 pKa = 3.68 SSDD513 pKa = 4.93 DD514 pKa = 3.53 GSIDD518 pKa = 4.4 INDD521 pKa = 4.91 LDD523 pKa = 4.57 LQVDD527 pKa = 3.93 DD528 pKa = 4.06 TTDD531 pKa = 3.2 AVEE534 pKa = 4.02 EE535 pKa = 4.23
Molecular weight: 58.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.923
Patrickios 0.998
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|R5VRR7|R5VRR7_9FIRM Putative DNA metabolism protein OS=Coprococcus eutactus CAG:665 OX=1263071 GN=BN751_02319 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.95 KK9 pKa = 7.58 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.07 VHH16 pKa = 5.95 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 KK29 pKa = 8.89 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 8.83 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2415
0
2415
797336
30
3567
330.2
36.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.279 ± 0.045
1.509 ± 0.022
6.923 ± 0.049
6.81 ± 0.051
3.843 ± 0.039
7.103 ± 0.044
1.58 ± 0.02
7.618 ± 0.052
6.976 ± 0.047
7.922 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.212 ± 0.032
4.724 ± 0.038
2.909 ± 0.028
2.717 ± 0.028
4.281 ± 0.041
6.243 ± 0.044
5.542 ± 0.051
7.416 ± 0.041
0.839 ± 0.017
4.543 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here