Streptococcus phage Javan497

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AJA8|A0A4D6AJA8_9CAUD Transcriptional activator OS=Streptococcus phage Javan497 OX=2548211 GN=Javan497_0022 PE=4 SV=1
MM1 pKa = 7.64NYY3 pKa = 10.44LEE5 pKa = 4.33YY6 pKa = 11.13ALAYY10 pKa = 10.27LEE12 pKa = 4.77RR13 pKa = 11.84EE14 pKa = 4.2LEE16 pKa = 4.54IIDD19 pKa = 4.43DD20 pKa = 3.92EE21 pKa = 4.88VIEE24 pKa = 4.2VEE26 pKa = 4.76LPGGDD31 pKa = 3.23WEE33 pKa = 4.69FVPNPYY39 pKa = 10.03YY40 pKa = 10.52EE41 pKa = 4.88EE42 pKa = 4.6GLHH45 pKa = 6.64DD46 pKa = 3.79SPYY49 pKa = 10.48YY50 pKa = 10.02RR51 pKa = 11.84SQVAKK56 pKa = 10.55DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AHH3|A0A4D6AHH3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan497 OX=2548211 GN=Javan497_0011 PE=4 SV=1
MM1 pKa = 7.32IEE3 pKa = 5.0KK4 pKa = 8.7YY5 pKa = 8.55TKK7 pKa = 10.41KK8 pKa = 10.57DD9 pKa = 2.72GTTAYY14 pKa = 10.65RR15 pKa = 11.84LRR17 pKa = 11.84AYY19 pKa = 10.71LGVDD23 pKa = 3.48PMTGKK28 pKa = 9.66QVRR31 pKa = 11.84TTRR34 pKa = 11.84QGFKK38 pKa = 9.38TEE40 pKa = 4.06RR41 pKa = 11.84EE42 pKa = 4.18AKK44 pKa = 8.09RR45 pKa = 11.84TEE47 pKa = 4.01VKK49 pKa = 10.68LVDD52 pKa = 3.62DD53 pKa = 5.01FQRR56 pKa = 11.84QGAWKK61 pKa = 10.31NNDD64 pKa = 2.62KK65 pKa = 8.55TTFDD69 pKa = 3.5DD70 pKa = 5.1AARR73 pKa = 11.84LWFEE77 pKa = 3.98QYY79 pKa = 11.18KK80 pKa = 8.56NTVKK84 pKa = 10.76PSTFLVNQNYY94 pKa = 9.38YY95 pKa = 10.64KK96 pKa = 10.0STIKK100 pKa = 10.34PHH102 pKa = 6.22IGKK105 pKa = 8.65SQISKK110 pKa = 7.62ITVMACQNLINHH122 pKa = 7.46LAQYY126 pKa = 8.0SACASYY132 pKa = 10.74ISVINRR138 pKa = 11.84IFKK141 pKa = 10.19FAVHH145 pKa = 6.62LGIIEE150 pKa = 4.38NNPMDD155 pKa = 3.51RR156 pKa = 11.84TIRR159 pKa = 11.84ARR161 pKa = 11.84CTYY164 pKa = 9.73AHH166 pKa = 6.98KK167 pKa = 11.03EE168 pKa = 3.59KK169 pKa = 10.24MFYY172 pKa = 10.59SKK174 pKa = 11.32EE175 pKa = 3.87EE176 pKa = 3.83LLIFLDD182 pKa = 3.65IVKK185 pKa = 10.25KK186 pKa = 10.63EE187 pKa = 4.05EE188 pKa = 4.17SLEE191 pKa = 3.75MLLIYY196 pKa = 10.48RR197 pKa = 11.84LLAYY201 pKa = 10.06GGLRR205 pKa = 11.84IGEE208 pKa = 4.13AVALYY213 pKa = 8.48DD214 pKa = 4.0TDD216 pKa = 5.82FDD218 pKa = 4.2FTNNTISITKK228 pKa = 9.07TLAQTKK234 pKa = 9.56AGKK237 pKa = 10.07VIQNPKK243 pKa = 7.57TKK245 pKa = 10.04KK246 pKa = 9.1SSRR249 pKa = 11.84TISMDD254 pKa = 3.19QEE256 pKa = 4.1TMRR259 pKa = 11.84LAKK262 pKa = 10.13EE263 pKa = 3.84YY264 pKa = 9.12MRR266 pKa = 11.84QSVRR270 pKa = 11.84PLHH273 pKa = 6.09GSFRR277 pKa = 11.84VFNLLTSTVRR287 pKa = 11.84RR288 pKa = 11.84RR289 pKa = 11.84LKK291 pKa = 9.44RR292 pKa = 11.84TVEE295 pKa = 3.72RR296 pKa = 11.84HH297 pKa = 4.98HH298 pKa = 6.73LKK300 pKa = 10.45KK301 pKa = 10.44INPHH305 pKa = 5.44GFRR308 pKa = 11.84HH309 pKa = 4.99THH311 pKa = 6.0ASLLFEE317 pKa = 5.22AGVPAKK323 pKa = 10.26LAQEE327 pKa = 4.26RR328 pKa = 11.84LGHH331 pKa = 5.98AKK333 pKa = 10.11ISITMDD339 pKa = 3.7LYY341 pKa = 10.51THH343 pKa = 7.22LSKK346 pKa = 11.23NQTDD350 pKa = 3.66NVAEE354 pKa = 4.12KK355 pKa = 10.42LADD358 pKa = 5.17FIAII362 pKa = 4.09

Molecular weight: 41.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12102

38

1460

242.0

27.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.147 ± 0.646

0.463 ± 0.106

6.148 ± 0.488

7.238 ± 0.617

3.685 ± 0.175

6.453 ± 0.434

1.231 ± 0.172

6.949 ± 0.377

8.651 ± 0.484

8.792 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.143

5.197 ± 0.255

2.661 ± 0.195

3.867 ± 0.277

4.338 ± 0.387

6.561 ± 0.721

6.313 ± 0.29

6.148 ± 0.194

0.942 ± 0.144

3.859 ± 0.427
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski