Mycobacterium phage Antsirabe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TMM2|A0A5J6TMM2_9CAUD Uncharacterized protein OS=Mycobacterium phage Antsirabe OX=2575610 GN=8 PE=4 SV=1
MM1 pKa = 6.53THH3 pKa = 6.11SPAMRR8 pKa = 11.84AALDD12 pKa = 3.99ALDD15 pKa = 4.17CAIVDD20 pKa = 3.52VFEE23 pKa = 6.13AIRR26 pKa = 11.84ADD28 pKa = 5.05DD29 pKa = 5.27DD30 pKa = 4.02DD31 pKa = 4.61TPADD35 pKa = 5.25AIPVDD40 pKa = 4.02AVLLIGVQHH49 pKa = 7.0IDD51 pKa = 2.98SDD53 pKa = 3.8DD54 pKa = 3.78DD55 pKa = 3.78ARR57 pKa = 11.84VGYY60 pKa = 8.4TEE62 pKa = 3.82VVPRR66 pKa = 11.84YY67 pKa = 8.46GAQPAYY73 pKa = 8.56ITRR76 pKa = 11.84GLLDD80 pKa = 4.29DD81 pKa = 4.3ARR83 pKa = 11.84KK84 pKa = 9.81LLDD87 pKa = 3.45QVADD91 pKa = 3.7QGDD94 pKa = 3.86DD95 pKa = 3.46RR96 pKa = 11.84PQGGG100 pKa = 3.28

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TH07|A0A5J6TH07_9CAUD Uncharacterized protein OS=Mycobacterium phage Antsirabe OX=2575610 GN=62 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.16NRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84LTVGAQPGQGRR17 pKa = 11.84SCGLRR22 pKa = 11.84CRR24 pKa = 11.84NHH26 pKa = 6.03LAVMCGRR33 pKa = 11.84SVGDD37 pKa = 3.67CLAAEE42 pKa = 4.53ALDD45 pKa = 4.04FGLGLSSLVGDD56 pKa = 3.71VSRR59 pKa = 11.84TCRR62 pKa = 11.84CSVVRR67 pKa = 11.84RR68 pKa = 11.84GRR70 pKa = 11.84LDD72 pKa = 3.58LNGPVWFAA80 pKa = 3.83

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14542

43

1325

220.3

23.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.189 ± 0.636

0.949 ± 0.195

7.289 ± 0.385

5.419 ± 0.421

2.978 ± 0.17

9.648 ± 0.701

1.891 ± 0.191

4.236 ± 0.207

3.301 ± 0.229

7.468 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.136

3.005 ± 0.164

6.271 ± 0.431

3.658 ± 0.17

7.104 ± 0.445

4.477 ± 0.288

6.01 ± 0.264

6.939 ± 0.212

2.056 ± 0.149

2.015 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski