Planktothrix agardhii (Oscillatoria agardhii)
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 147 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J1JMR7|A0A1J1JMR7_PLAAG TNase-like domain-containing protein OS=Planktothrix agardhii OX=1160 GN=PLAM_mp0046 PE=4 SV=1
MM1 pKa = 7.71 NEE3 pKa = 5.35 LNGQWLEE10 pKa = 4.38 CGGKK14 pKa = 9.18 PCFCPDD20 pKa = 3.85 NSPSPYY26 pKa = 10.42 DD27 pKa = 3.92 YY28 pKa = 11.36 LWDD31 pKa = 5.11 DD32 pKa = 4.85 DD33 pKa = 4.6 IEE35 pKa = 6.13 DD36 pKa = 3.55 IEE38 pKa = 4.42 EE39 pKa = 4.03 NKK41 pKa = 10.43 EE42 pKa = 3.94 VIEE45 pKa = 4.29 FRR47 pKa = 11.84 EE48 pKa = 4.09 KK49 pKa = 10.59 CKK51 pKa = 10.75 LISRR55 pKa = 11.84 YY56 pKa = 9.94 SSLIEE61 pKa = 4.41 PFDD64 pKa = 3.87 SAQEE68 pKa = 4.03 SPIEE72 pKa = 4.32 FEE74 pKa = 4.09 IEE76 pKa = 4.02 SNSRR80 pKa = 11.84 YY81 pKa = 10.54 DD82 pKa = 3.46 EE83 pKa = 4.22 FF84 pKa = 6.55
Molecular weight: 9.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.961
IPC_protein 3.872
Toseland 3.694
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.719
Rodwell 3.706
Grimsley 3.605
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.075
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.973
Patrickios 1.863
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A1J1JL52|A0A1J1JL52_PLAAG Uncharacterized protein OS=Planktothrix agardhii OX=1160 GN=PLAM_mp0009 PE=4 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 EE3 pKa = 3.42 IFVFFFIRR11 pKa = 11.84 WVLLTISDD19 pKa = 4.4 LSFFYY24 pKa = 10.68 HH25 pKa = 7.06 KK26 pKa = 10.26 SLHH29 pKa = 5.98 SLYY32 pKa = 10.59 CLVFSLVCRR41 pKa = 11.84 PVSFISLVLLIAIAYY56 pKa = 9.58 SSATIQGQQIKK67 pKa = 10.38 RR68 pKa = 11.84 KK69 pKa = 9.8 GIQKK73 pKa = 9.68 YY74 pKa = 7.72 IARR77 pKa = 11.84 IKK79 pKa = 9.98 EE80 pKa = 3.65 YY81 pKa = 10.47 GRR83 pKa = 11.84 IEE85 pKa = 3.92 RR86 pKa = 11.84 RR87 pKa = 11.84 HH88 pKa = 5.16 SSFYY92 pKa = 10.3 IGLYY96 pKa = 8.34 GQTWVNFKK104 pKa = 10.02 DD105 pKa = 3.38 VCMDD109 pKa = 4.65 LVTKK113 pKa = 10.52 LMKK116 pKa = 10.66 LNRR119 pKa = 11.84 NKK121 pKa = 10.3 CKK123 pKa = 10.45 YY124 pKa = 7.96 YY125 pKa = 9.95 QQGLRR130 pKa = 11.84 AMRR133 pKa = 11.84 LIEE136 pKa = 4.35 SVLL139 pKa = 3.53
Molecular weight: 16.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.589
IPC_protein 9.78
Toseland 9.999
ProMoST 9.78
Dawson 10.248
Bjellqvist 9.984
Wikipedia 10.452
Rodwell 10.57
Grimsley 10.335
Solomon 10.277
Lehninger 10.233
Nozaki 10.058
DTASelect 9.955
Thurlkill 10.087
EMBOSS 10.409
Sillero 10.175
Patrickios 9.882
IPC_peptide 10.277
IPC2_peptide 8.96
IPC2.peptide.svr19 8.458
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
147
0
147
44004
44
2995
299.3
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.531 ± 0.171
1.279 ± 0.075
5.3 ± 0.118
7.113 ± 0.149
3.877 ± 0.102
5.831 ± 0.19
2.095 ± 0.084
6.99 ± 0.187
5.849 ± 0.211
10.715 ± 0.19
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.773 ± 0.073
4.738 ± 0.127
4.734 ± 0.143
5.45 ± 0.181
5.031 ± 0.212
6.547 ± 0.132
5.259 ± 0.136
5.899 ± 0.135
1.559 ± 0.063
3.429 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here