Amino acid dipepetide frequency for bacterium 336/3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.586AlaAla: 2.586 ± 0.06	0.724AlaCys: 0.724 ± 0.025	3.005AlaAsp: 3.005 ± 0.048	3.995AlaGlu: 3.995 ± 0.063	3.378AlaPhe: 3.378 ± 0.051	3.966AlaGly: 3.966 ± 0.069	1.034AlaHis: 1.034 ± 0.03	5.067AlaIle: 5.067 ± 0.069	4.861AlaLys: 4.861 ± 0.074	6.118AlaLeu: 6.118 ± 0.079	1.387AlaMet: 1.387 ± 0.036	3.331AlaAsn: 3.331 ± 0.053	1.632AlaPro: 1.632 ± 0.042	3.055AlaGln: 3.055 ± 0.052	1.846AlaArg: 1.846 ± 0.038	4.004AlaSer: 4.004 ± 0.065	3.58AlaThr: 3.58 ± 0.063	3.368AlaVal: 3.368 ± 0.055	0.692AlaTrp: 0.692 ± 0.024	2.599AlaTyr: 2.599 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.4CysAla: 0.4 ± 0.019	0.1CysCys: 0.1 ± 0.01	0.368CysAsp: 0.368 ± 0.018	0.457CysGlu: 0.457 ± 0.02	0.555CysPhe: 0.555 ± 0.025	0.606CysGly: 0.606 ± 0.025	0.165CysHis: 0.165 ± 0.012	0.715CysIle: 0.715 ± 0.026	0.66CysLys: 0.66 ± 0.021	0.795CysLeu: 0.795 ± 0.026	0.184CysMet: 0.184 ± 0.012	0.429CysAsn: 0.429 ± 0.018	0.344CysPro: 0.344 ± 0.019	0.317CysGln: 0.317 ± 0.016	0.239CysArg: 0.239 ± 0.013	0.592CysSer: 0.592 ± 0.022	0.424CysThr: 0.424 ± 0.02	0.506CysVal: 0.506 ± 0.019	0.083CysTrp: 0.083 ± 0.008	0.345CysTyr: 0.345 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.126AspAla: 3.126 ± 0.058	0.409AspCys: 0.409 ± 0.019	2.276AspAsp: 2.276 ± 0.047	3.399AspGlu: 3.399 ± 0.063	3.523AspPhe: 3.523 ± 0.054	2.92AspGly: 2.92 ± 0.061	0.549AspHis: 0.549 ± 0.023	4.392AspIle: 4.392 ± 0.064	4.558AspLys: 4.558 ± 0.073	4.786AspLeu: 4.786 ± 0.067	1.093AspMet: 1.093 ± 0.027	2.805AspAsn: 2.805 ± 0.05	1.196AspPro: 1.196 ± 0.029	1.014AspGln: 1.014 ± 0.028	1.409AspArg: 1.409 ± 0.039	2.958AspSer: 2.958 ± 0.045	2.675AspThr: 2.675 ± 0.051	2.966AspVal: 2.966 ± 0.053	0.701AspTrp: 0.701 ± 0.026	2.399AspTyr: 2.399 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	4.307GluAla: 4.307 ± 0.068	0.395GluCys: 0.395 ± 0.018	3.159GluAsp: 3.159 ± 0.048	4.952GluGlu: 4.952 ± 0.077	2.84GluPhe: 2.84 ± 0.044	3.484GluGly: 3.484 ± 0.053	1.188GluHis: 1.188 ± 0.03	6.006GluIle: 6.006 ± 0.079	7.629GluLys: 7.629 ± 0.111	5.983GluLeu: 5.983 ± 0.079	1.692GluMet: 1.692 ± 0.038	4.963GluAsn: 4.963 ± 0.065	1.669GluPro: 1.669 ± 0.034	3.046GluGln: 3.046 ± 0.053	2.454GluArg: 2.454 ± 0.051	3.383GluSer: 3.383 ± 0.059	3.485GluThr: 3.485 ± 0.057	3.891GluVal: 3.891 ± 0.063	0.705GluTrp: 0.705 ± 0.024	2.827GluTyr: 2.827 ± 0.052	0.001GluXaa: 0.001 ± 0.001
Phe	3.214PheAla: 3.214 ± 0.051	0.535PheCys: 0.535 ± 0.022	2.85PheAsp: 2.85 ± 0.05	3.69PheGlu: 3.69 ± 0.065	3.19PhePhe: 3.19 ± 0.059	3.387PheGly: 3.387 ± 0.053	0.855PheHis: 0.855 ± 0.029	4.105PheIle: 4.105 ± 0.06	3.788PheLys: 3.788 ± 0.049	5.575PheLeu: 5.575 ± 0.082	1.119PheMet: 1.119 ± 0.035	2.739PheAsn: 2.739 ± 0.046	1.613PhePro: 1.613 ± 0.033	1.976PheGln: 1.976 ± 0.041	1.757PheArg: 1.757 ± 0.036	4.028PheSer: 4.028 ± 0.068	3.064PheThr: 3.064 ± 0.052	3.271PheVal: 3.271 ± 0.051	0.775PheTrp: 0.775 ± 0.028	2.48PheTyr: 2.48 ± 0.051	0.001PheXaa: 0.001 ± 0.001
Gly	3.592GlyAla: 3.592 ± 0.061	0.609GlyCys: 0.609 ± 0.022	2.75GlyAsp: 2.75 ± 0.054	3.351GlyGlu: 3.351 ± 0.057	3.559GlyPhe: 3.559 ± 0.055	4.196GlyGly: 4.196 ± 0.089	0.949GlyHis: 0.949 ± 0.029	5.107GlyIle: 5.107 ± 0.064	5.373GlyLys: 5.373 ± 0.073	5.499GlyLeu: 5.499 ± 0.061	1.385GlyMet: 1.385 ± 0.033	3.403GlyAsn: 3.403 ± 0.07	0.691GlyPro: 0.691 ± 0.024	1.87GlyGln: 1.87 ± 0.042	1.925GlyArg: 1.925 ± 0.048	3.677GlySer: 3.677 ± 0.073	3.576GlyThr: 3.576 ± 0.082	4.153GlyVal: 4.153 ± 0.069	0.748GlyTrp: 0.748 ± 0.031	2.646GlyTyr: 2.646 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.84HisAla: 0.84 ± 0.026	0.174HisCys: 0.174 ± 0.011	0.654HisAsp: 0.654 ± 0.024	0.941HisGlu: 0.941 ± 0.026	1.234HisPhe: 1.234 ± 0.035	0.849HisGly: 0.849 ± 0.024	0.544HisHis: 0.544 ± 0.022	1.619HisIle: 1.619 ± 0.039	1.314HisLys: 1.314 ± 0.031	2.01HisLeu: 2.01 ± 0.046	0.207HisMet: 0.207 ± 0.012	0.866HisAsn: 0.866 ± 0.025	0.866HisPro: 0.866 ± 0.027	0.957HisGln: 0.957 ± 0.028	0.565HisArg: 0.565 ± 0.022	1.03HisSer: 1.03 ± 0.029	1.065HisThr: 1.065 ± 0.031	0.776HisVal: 0.776 ± 0.027	0.241HisTrp: 0.241 ± 0.013	0.92HisTyr: 0.92 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.628IleAla: 5.628 ± 0.065	0.758IleCys: 0.758 ± 0.027	4.422IleAsp: 4.422 ± 0.067	5.972IleGlu: 5.972 ± 0.082	4.322IlePhe: 4.322 ± 0.073	4.796IleGly: 4.796 ± 0.064	1.617IleHis: 1.617 ± 0.039	6.198IleIle: 6.198 ± 0.09	5.999IleLys: 5.999 ± 0.079	8.136IleLeu: 8.136 ± 0.103	1.386IleMet: 1.386 ± 0.032	4.361IleAsn: 4.361 ± 0.069	3.181IlePro: 3.181 ± 0.048	3.491IleGln: 3.491 ± 0.059	2.98IleArg: 2.98 ± 0.045	5.786IleSer: 5.786 ± 0.075	4.689IleThr: 4.689 ± 0.069	4.777IleVal: 4.777 ± 0.065	0.918IleTrp: 0.918 ± 0.029	3.238IleTyr: 3.238 ± 0.051	0.001IleXaa: 0.001 ± 0.001
Lys	5.241LysAla: 5.241 ± 0.077	0.39LysCys: 0.39 ± 0.019	4.742LysAsp: 4.742 ± 0.065	6.79LysGlu: 6.79 ± 0.092	2.991LysPhe: 2.991 ± 0.052	4.715LysGly: 4.715 ± 0.065	1.47LysHis: 1.47 ± 0.035	7.484LysIle: 7.484 ± 0.084	8.416LysLys: 8.416 ± 0.112	6.804LysLeu: 6.804 ± 0.078	2.375LysMet: 2.375 ± 0.049	6.323LysAsn: 6.323 ± 0.08	2.678LysPro: 2.678 ± 0.052	3.523LysGln: 3.523 ± 0.051	2.743LysArg: 2.743 ± 0.047	4.617LysSer: 4.617 ± 0.064	4.914LysThr: 4.914 ± 0.062	4.765LysVal: 4.765 ± 0.071	0.821LysTrp: 0.821 ± 0.027	3.508LysTyr: 3.508 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	6.063LeuAla: 6.063 ± 0.08	0.83LeuCys: 0.83 ± 0.028	4.838LeuAsp: 4.838 ± 0.065	7.068LeuGlu: 7.068 ± 0.086	4.895LeuPhe: 4.895 ± 0.079	5.962LeuGly: 5.962 ± 0.081	1.71LeuHis: 1.71 ± 0.041	7.019LeuIle: 7.019 ± 0.084	8.152LeuLys: 8.152 ± 0.098	9.042LeuLeu: 9.042 ± 0.117	2.219LeuMet: 2.219 ± 0.045	5.165LeuAsn: 5.165 ± 0.062	3.74LeuPro: 3.74 ± 0.06	4.34LeuGln: 4.34 ± 0.062	3.229LeuArg: 3.229 ± 0.053	6.622LeuSer: 6.622 ± 0.081	4.647LeuThr: 4.647 ± 0.061	5.328LeuVal: 5.328 ± 0.075	0.983LeuTrp: 0.983 ± 0.03	3.64LeuTyr: 3.64 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	1.529MetAla: 1.529 ± 0.032	0.149MetCys: 0.149 ± 0.011	1.036MetAsp: 1.036 ± 0.028	1.358MetGlu: 1.358 ± 0.032	0.899MetPhe: 0.899 ± 0.029	1.45MetGly: 1.45 ± 0.036	0.348MetHis: 0.348 ± 0.017	1.699MetIle: 1.699 ± 0.043	2.146MetLys: 2.146 ± 0.04	2.154MetLeu: 2.154 ± 0.05	0.634MetMet: 0.634 ± 0.025	1.267MetAsn: 1.267 ± 0.033	1.005MetPro: 1.005 ± 0.029	0.997MetGln: 0.997 ± 0.028	0.813MetArg: 0.813 ± 0.027	1.315MetSer: 1.315 ± 0.034	1.024MetThr: 1.024 ± 0.029	1.185MetVal: 1.185 ± 0.031	0.189MetTrp: 0.189 ± 0.013	0.768MetTyr: 0.768 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.619AsnAla: 3.619 ± 0.063	0.444AsnCys: 0.444 ± 0.019	2.56AsnAsp: 2.56 ± 0.047	3.39AsnGlu: 3.39 ± 0.06	3.262AsnPhe: 3.262 ± 0.055	3.327AsnGly: 3.327 ± 0.076	1.089AsnHis: 1.089 ± 0.028	5.463AsnIle: 5.463 ± 0.08	4.456AsnLys: 4.456 ± 0.065	5.965AsnLeu: 5.965 ± 0.073	1.134AsnMet: 1.134 ± 0.031	3.698AsnAsn: 3.698 ± 0.085	2.925AsnPro: 2.925 ± 0.06	2.744AsnGln: 2.744 ± 0.052	1.934AsnArg: 1.934 ± 0.042	3.397AsnSer: 3.397 ± 0.066	3.759AsnThr: 3.759 ± 0.069	3.062AsnVal: 3.062 ± 0.055	0.762AsnTrp: 0.762 ± 0.027	2.79AsnTyr: 2.79 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	1.962ProAla: 1.962 ± 0.039	0.241ProCys: 0.241 ± 0.013	1.841ProAsp: 1.841 ± 0.04	2.564ProGlu: 2.564 ± 0.052	1.882ProPhe: 1.882 ± 0.043	0.991ProGly: 0.991 ± 0.035	0.685ProHis: 0.685 ± 0.024	2.849ProIle: 2.849 ± 0.049	2.786ProLys: 2.786 ± 0.05	2.917ProLeu: 2.917 ± 0.053	0.66ProMet: 0.66 ± 0.021	2.268ProAsn: 2.268 ± 0.051	0.853ProPro: 0.853 ± 0.03	1.57ProGln: 1.57 ± 0.037	0.906ProArg: 0.906 ± 0.026	2.152ProSer: 2.152 ± 0.05	2.162ProThr: 2.162 ± 0.058	1.972ProVal: 1.972 ± 0.04	0.275ProTrp: 0.275 ± 0.014	1.4ProTyr: 1.4 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.627GlnAla: 2.627 ± 0.049	0.216GlnCys: 0.216 ± 0.014	2.073GlnAsp: 2.073 ± 0.042	3.468GlnGlu: 3.468 ± 0.058	1.659GlnPhe: 1.659 ± 0.035	2.19GlnGly: 2.19 ± 0.046	0.616GlnHis: 0.616 ± 0.023	3.748GlnIle: 3.748 ± 0.055	4.662GlnLys: 4.662 ± 0.078	3.195GlnLeu: 3.195 ± 0.055	1.02GlnMet: 1.02 ± 0.029	3.204GlnAsn: 3.204 ± 0.049	1.125GlnPro: 1.125 ± 0.03	1.606GlnGln: 1.606 ± 0.041	1.388GlnArg: 1.388 ± 0.032	2.226GlnSer: 2.226 ± 0.044	2.573GlnThr: 2.573 ± 0.043	2.271GlnVal: 2.271 ± 0.041	0.396GlnTrp: 0.396 ± 0.017	1.585GlnTyr: 1.585 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	1.73ArgAla: 1.73 ± 0.044	0.21ArgCys: 0.21 ± 0.014	1.487ArgAsp: 1.487 ± 0.032	2.066ArgGlu: 2.066 ± 0.044	1.978ArgPhe: 1.978 ± 0.04	1.711ArgGly: 1.711 ± 0.039	0.549ArgHis: 0.549 ± 0.02	2.874ArgIle: 2.874 ± 0.049	3.036ArgLys: 3.036 ± 0.052	3.241ArgLeu: 3.241 ± 0.052	0.856ArgMet: 0.856 ± 0.028	2.066ArgAsn: 2.066 ± 0.043	1.05ArgPro: 1.05 ± 0.029	1.233ArgGln: 1.233 ± 0.029	1.294ArgArg: 1.294 ± 0.031	1.646ArgSer: 1.646 ± 0.034	1.74ArgThr: 1.74 ± 0.041	1.971ArgVal: 1.971 ± 0.035	0.4ArgTrp: 0.4 ± 0.016	1.632ArgTyr: 1.632 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.193SerAla: 3.193 ± 0.057	0.588SerCys: 0.588 ± 0.022	2.786SerAsp: 2.786 ± 0.048	3.792SerGlu: 3.792 ± 0.054	3.967SerPhe: 3.967 ± 0.064	3.874SerGly: 3.874 ± 0.074	1.089SerHis: 1.089 ± 0.029	5.334SerIle: 5.334 ± 0.067	4.995SerLys: 4.995 ± 0.07	6.438SerLeu: 6.438 ± 0.081	1.313SerMet: 1.313 ± 0.031	3.461SerAsn: 3.461 ± 0.062	2.289SerPro: 2.289 ± 0.047	2.654SerGln: 2.654 ± 0.062	1.777SerArg: 1.777 ± 0.034	4.008SerSer: 4.008 ± 0.073	3.221SerThr: 3.221 ± 0.059	3.859SerVal: 3.859 ± 0.058	0.786SerTrp: 0.786 ± 0.025	2.821SerTyr: 2.821 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	3.253ThrAla: 3.253 ± 0.064	0.408ThrCys: 0.408 ± 0.019	2.941ThrAsp: 2.941 ± 0.046	3.567ThrGlu: 3.567 ± 0.048	3.107ThrPhe: 3.107 ± 0.058	3.512ThrGly: 3.512 ± 0.071	1.095ThrHis: 1.095 ± 0.028	4.617ThrIle: 4.617 ± 0.061	4.003ThrLys: 4.003 ± 0.063	5.625ThrLeu: 5.625 ± 0.065	0.939ThrMet: 0.939 ± 0.024	3.273ThrAsn: 3.273 ± 0.061	2.509ThrPro: 2.509 ± 0.052	2.47ThrGln: 2.47 ± 0.047	1.604ThrArg: 1.604 ± 0.038	3.553ThrSer: 3.553 ± 0.067	3.505ThrThr: 3.505 ± 0.076	3.022ThrVal: 3.022 ± 0.063	0.653ThrTrp: 0.653 ± 0.022	2.336ThrTyr: 2.336 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.062ValAla: 4.062 ± 0.061	0.605ValCys: 0.605 ± 0.022	2.704ValAsp: 2.704 ± 0.041	3.815ValGlu: 3.815 ± 0.055	3.481ValPhe: 3.481 ± 0.053	3.928ValGly: 3.928 ± 0.06	0.937ValHis: 0.937 ± 0.023	4.349ValIle: 4.349 ± 0.072	4.241ValLys: 4.241 ± 0.066	5.702ValLeu: 5.702 ± 0.076	1.311ValMet: 1.311 ± 0.033	2.994ValAsn: 2.994 ± 0.06	1.888ValPro: 1.888 ± 0.037	2.001ValGln: 2.001 ± 0.043	2.006ValArg: 2.006 ± 0.044	3.974ValSer: 3.974 ± 0.059	2.843ValThr: 2.843 ± 0.059	3.949ValVal: 3.949 ± 0.065	0.746ValTrp: 0.746 ± 0.027	2.461ValTyr: 2.461 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.722TrpAla: 0.722 ± 0.028	0.1TrpCys: 0.1 ± 0.01	0.603TrpAsp: 0.603 ± 0.025	0.708TrpGlu: 0.708 ± 0.025	0.599TrpPhe: 0.599 ± 0.023	0.778TrpGly: 0.778 ± 0.027	0.257TrpHis: 0.257 ± 0.014	0.955TrpIle: 0.955 ± 0.033	0.927TrpLys: 0.927 ± 0.03	1.264TrpLeu: 1.264 ± 0.041	0.311TrpMet: 0.311 ± 0.016	0.712TrpAsn: 0.712 ± 0.03	0.066TrpPro: 0.066 ± 0.007	0.637TrpGln: 0.637 ± 0.023	0.416TrpArg: 0.416 ± 0.018	0.62TrpSer: 0.62 ± 0.021	0.55TrpThr: 0.55 ± 0.021	0.766TrpVal: 0.766 ± 0.022	0.159TrpTrp: 0.159 ± 0.011	0.472TrpTyr: 0.472 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.536TyrAla: 2.536 ± 0.052	0.4TyrCys: 0.4 ± 0.018	2.058TyrAsp: 2.058 ± 0.05	2.715TyrGlu: 2.715 ± 0.048	2.69TyrPhe: 2.69 ± 0.047	2.418TyrGly: 2.418 ± 0.041	0.925TyrHis: 0.925 ± 0.031	3.054TyrIle: 3.054 ± 0.048	3.312TyrLys: 3.312 ± 0.054	4.244TyrLeu: 4.244 ± 0.061	0.739TyrMet: 0.739 ± 0.025	2.455TyrAsn: 2.455 ± 0.045	1.616TyrPro: 1.616 ± 0.034	2.345TyrGln: 2.345 ± 0.046	1.481TyrArg: 1.481 ± 0.036	2.654TyrSer: 2.654 ± 0.045	2.498TyrThr: 2.498 ± 0.052	2.138TyrVal: 2.138 ± 0.039	0.591TyrTrp: 0.591 ± 0.022	2.06TyrTyr: 2.06 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.007XaaXaa: 0.007 ± 0.004

Statistics based on 4024 proteins (1296309 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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