Burkholderiales bacterium PBB1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257DFM6|A0A257DFM6_9BURK YHS domain protein OS=Burkholderiales bacterium PBB1 OX=2015563 GN=CFE40_03485 PE=4 SV=1
MM1 pKa = 8.1VEE3 pKa = 4.04VSGGNYY9 pKa = 9.58LDD11 pKa = 4.23EE12 pKa = 4.55ATSVSTALSAPMRR25 pKa = 11.84VVINANGGSVTGVVTPLTTLAYY47 pKa = 8.48TYY49 pKa = 11.47AFGTTGTPTSTAYY62 pKa = 9.22NTVATNLATQLQIPGVNIATTAPVVTGTVNDD93 pKa = 3.8YY94 pKa = 11.15GRR96 pKa = 11.84LLAGISTYY104 pKa = 10.95LQLNSVSLQSLISTALDD121 pKa = 3.57EE122 pKa = 4.34VDD124 pKa = 3.31SAQFSVAFTTAYY136 pKa = 8.28NTANPDD142 pKa = 3.06HH143 pKa = 6.75TLTFTFDD150 pKa = 3.24GSKK153 pKa = 10.93LNVSGTGVGGGSGTCGVNVQGTLTVGGNSVPLNLNYY189 pKa = 10.4CINGIAADD197 pKa = 3.92SCTSGNSSLSQALSGQSGLAGAANLNYY224 pKa = 9.88AYY226 pKa = 10.82SPTCVANPDD235 pKa = 3.35FTINLNN241 pKa = 3.55

Molecular weight: 24.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257DB16|A0A257DB16_9BURK Uncharacterized protein OS=Burkholderiales bacterium PBB1 OX=2015563 GN=CFE40_11045 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.73QASKK9 pKa = 7.81TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2893

0

2893

996319

29

12183

344.4

37.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.891 ± 0.062

0.931 ± 0.019

5.504 ± 0.035

5.075 ± 0.05

3.313 ± 0.028

8.111 ± 0.052

2.289 ± 0.03

4.282 ± 0.035

2.98 ± 0.045

10.8 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.027

2.519 ± 0.036

5.321 ± 0.043

3.888 ± 0.024

7.147 ± 0.054

5.779 ± 0.05

5.443 ± 0.058

7.873 ± 0.035

1.432 ± 0.018

2.117 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski