Amino acid dipepetide frequency for Ralstonia phage RP31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.164AlaAla: 11.164 ± 1.002
0.76AlaCys: 0.76 ± 0.106
5.129AlaAsp: 5.129 ± 0.241
6.276AlaGlu: 6.276 ± 0.453
3.234AlaPhe: 3.234 ± 0.213
5.745AlaGly: 5.745 ± 0.453
1.497AlaHis: 1.497 ± 0.134
5.419AlaIle: 5.419 ± 0.292
5.19AlaLys: 5.19 ± 0.324
7.869AlaLeu: 7.869 ± 0.369
3.09AlaMet: 3.09 ± 0.201
3.669AlaAsn: 3.669 ± 0.24
4.417AlaPro: 4.417 ± 0.611
3.319AlaGln: 3.319 ± 0.276
4.103AlaArg: 4.103 ± 0.216
5.54AlaSer: 5.54 ± 0.335
5.033AlaThr: 5.033 ± 0.276
6.879AlaVal: 6.879 ± 0.336
0.965AlaTrp: 0.965 ± 0.111
3.174AlaTyr: 3.174 ± 0.212
0.0AlaXaa: 0.0 ± 0.0
Cys
0.76CysAla: 0.76 ± 0.105
0.193CysCys: 0.193 ± 0.047
0.591CysAsp: 0.591 ± 0.093
0.64CysGlu: 0.64 ± 0.1
0.41CysPhe: 0.41 ± 0.075
0.543CysGly: 0.543 ± 0.088
0.193CysHis: 0.193 ± 0.045
0.543CysIle: 0.543 ± 0.08
0.531CysLys: 0.531 ± 0.08
0.76CysLeu: 0.76 ± 0.105
0.241CysMet: 0.241 ± 0.046
0.447CysAsn: 0.447 ± 0.069
0.338CysPro: 0.338 ± 0.07
0.422CysGln: 0.422 ± 0.075
0.591CysArg: 0.591 ± 0.083
0.567CysSer: 0.567 ± 0.087
0.603CysThr: 0.603 ± 0.081
0.76CysVal: 0.76 ± 0.089
0.133CysTrp: 0.133 ± 0.044
0.338CysTyr: 0.338 ± 0.067
0.0CysXaa: 0.0 ± 0.0
Asp
5.359AspAla: 5.359 ± 0.273
0.555AspCys: 0.555 ± 0.086
3.5AspAsp: 3.5 ± 0.215
3.959AspGlu: 3.959 ± 0.247
2.764AspPhe: 2.764 ± 0.198
4.49AspGly: 4.49 ± 0.26
1.448AspHis: 1.448 ± 0.138
3.572AspIle: 3.572 ± 0.235
3.355AspLys: 3.355 ± 0.208
5.479AspLeu: 5.479 ± 0.24
1.762AspMet: 1.762 ± 0.16
2.595AspAsn: 2.595 ± 0.186
3.078AspPro: 3.078 ± 0.171
2.691AspGln: 2.691 ± 0.215
2.848AspArg: 2.848 ± 0.163
3.186AspSer: 3.186 ± 0.191
3.44AspThr: 3.44 ± 0.183
3.934AspVal: 3.934 ± 0.251
0.857AspTrp: 0.857 ± 0.113
2.39AspTyr: 2.39 ± 0.177
0.0AspXaa: 0.0 ± 0.0
Glu
6.3GluAla: 6.3 ± 0.388
0.616GluCys: 0.616 ± 0.071
3.524GluAsp: 3.524 ± 0.206
4.236GluGlu: 4.236 ± 0.275
2.534GluPhe: 2.534 ± 0.178
3.452GluGly: 3.452 ± 0.247
1.412GluHis: 1.412 ± 0.141
3.596GluIle: 3.596 ± 0.182
3.669GluLys: 3.669 ± 0.216
6.348GluLeu: 6.348 ± 0.272
1.726GluMet: 1.726 ± 0.134
2.607GluAsn: 2.607 ± 0.187
2.003GluPro: 2.003 ± 0.173
3.319GluGln: 3.319 ± 0.239
3.778GluArg: 3.778 ± 0.232
3.596GluSer: 3.596 ± 0.213
3.512GluThr: 3.512 ± 0.229
4.646GluVal: 4.646 ± 0.29
0.845GluTrp: 0.845 ± 0.11
2.281GluTyr: 2.281 ± 0.19
0.0GluXaa: 0.0 ± 0.0
Phe
2.848PheAla: 2.848 ± 0.211
0.603PheCys: 0.603 ± 0.083
3.21PheAsp: 3.21 ± 0.174
2.74PheGlu: 2.74 ± 0.198
1.883PhePhe: 1.883 ± 0.144
2.643PheGly: 2.643 ± 0.189
1.074PheHis: 1.074 ± 0.124
2.136PheIle: 2.136 ± 0.149
2.522PheLys: 2.522 ± 0.178
3.331PheLeu: 3.331 ± 0.225
0.905PheMet: 0.905 ± 0.103
2.148PheAsn: 2.148 ± 0.164
1.665PhePro: 1.665 ± 0.126
1.243PheGln: 1.243 ± 0.12
2.076PheArg: 2.076 ± 0.191
2.691PheSer: 2.691 ± 0.187
2.752PheThr: 2.752 ± 0.179
3.271PheVal: 3.271 ± 0.202
0.519PheTrp: 0.519 ± 0.08
1.557PheTyr: 1.557 ± 0.149
0.0PheXaa: 0.0 ± 0.0
Gly
5.19GlyAla: 5.19 ± 0.426
0.567GlyCys: 0.567 ± 0.092
3.959GlyAsp: 3.959 ± 0.446
4.369GlyGlu: 4.369 ± 0.349
2.8GlyPhe: 2.8 ± 0.186
4.803GlyGly: 4.803 ± 0.4
1.207GlyHis: 1.207 ± 0.121
3.862GlyIle: 3.862 ± 0.239
3.959GlyLys: 3.959 ± 0.233
5.829GlyLeu: 5.829 ± 0.282
2.04GlyMet: 2.04 ± 0.187
3.078GlyAsn: 3.078 ± 0.233
1.822GlyPro: 1.822 ± 0.344
2.245GlyGln: 2.245 ± 0.184
2.872GlyArg: 2.872 ± 0.179
4.429GlySer: 4.429 ± 0.261
3.91GlyThr: 3.91 ± 0.272
4.465GlyVal: 4.465 ± 0.277
0.965GlyTrp: 0.965 ± 0.109
2.522GlyTyr: 2.522 ± 0.174
0.0GlyXaa: 0.0 ± 0.0
His
1.762HisAla: 1.762 ± 0.16
0.278HisCys: 0.278 ± 0.06
0.833HisAsp: 0.833 ± 0.099
1.159HisGlu: 1.159 ± 0.156
1.074HisPhe: 1.074 ± 0.102
1.581HisGly: 1.581 ± 0.137
0.7HisHis: 0.7 ± 0.089
1.279HisIle: 1.279 ± 0.151
1.026HisLys: 1.026 ± 0.12
1.859HisLeu: 1.859 ± 0.164
0.531HisMet: 0.531 ± 0.073
0.772HisAsn: 0.772 ± 0.093
1.243HisPro: 1.243 ± 0.127
0.917HisGln: 0.917 ± 0.131
1.159HisArg: 1.159 ± 0.119
1.255HisSer: 1.255 ± 0.13
0.978HisThr: 0.978 ± 0.106
1.364HisVal: 1.364 ± 0.13
0.398HisTrp: 0.398 ± 0.072
0.917HisTyr: 0.917 ± 0.112
0.0HisXaa: 0.0 ± 0.0
Ile
5.636IleAla: 5.636 ± 0.283
0.471IleCys: 0.471 ± 0.071
4.067IleAsp: 4.067 ± 0.214
3.5IleGlu: 3.5 ± 0.186
1.883IlePhe: 1.883 ± 0.176
3.283IleGly: 3.283 ± 0.211
1.11IleHis: 1.11 ± 0.106
2.571IleIle: 2.571 ± 0.188
3.198IleLys: 3.198 ± 0.179
3.91IleLeu: 3.91 ± 0.196
1.038IleMet: 1.038 ± 0.115
2.896IleAsn: 2.896 ± 0.223
2.933IlePro: 2.933 ± 0.222
2.353IleGln: 2.353 ± 0.216
3.234IleArg: 3.234 ± 0.203
3.657IleSer: 3.657 ± 0.226
3.898IleThr: 3.898 ± 0.205
3.633IleVal: 3.633 ± 0.232
0.459IleTrp: 0.459 ± 0.072
1.665IleTyr: 1.665 ± 0.171
0.0IleXaa: 0.0 ± 0.0
Lys
4.924LysAla: 4.924 ± 0.317
0.434LysCys: 0.434 ± 0.066
3.56LysAsp: 3.56 ± 0.2
4.164LysGlu: 4.164 ± 0.283
2.559LysPhe: 2.559 ± 0.178
3.379LysGly: 3.379 ± 0.351
1.328LysHis: 1.328 ± 0.159
3.343LysIle: 3.343 ± 0.209
3.295LysLys: 3.295 ± 0.213
4.912LysLeu: 4.912 ± 0.306
1.46LysMet: 1.46 ± 0.141
2.486LysAsn: 2.486 ± 0.192
2.245LysPro: 2.245 ± 0.211
2.317LysGln: 2.317 ± 0.146
2.788LysArg: 2.788 ± 0.183
2.86LysSer: 2.86 ± 0.197
3.053LysThr: 3.053 ± 0.186
3.464LysVal: 3.464 ± 0.197
0.543LysTrp: 0.543 ± 0.081
1.75LysTyr: 1.75 ± 0.155
0.0LysXaa: 0.0 ± 0.0
Leu
7.434LeuAla: 7.434 ± 0.324
0.724LeuCys: 0.724 ± 0.083
5.322LeuAsp: 5.322 ± 0.294
5.817LeuGlu: 5.817 ± 0.282
3.572LeuPhe: 3.572 ± 0.228
5.346LeuGly: 5.346 ± 0.268
1.81LeuHis: 1.81 ± 0.155
4.586LeuIle: 4.586 ± 0.243
4.96LeuLys: 4.96 ± 0.294
6.903LeuLeu: 6.903 ± 0.345
2.378LeuMet: 2.378 ± 0.158
4.103LeuAsn: 4.103 ± 0.213
4.321LeuPro: 4.321 ± 0.221
3.536LeuGln: 3.536 ± 0.238
4.996LeuArg: 4.996 ± 0.239
6.215LeuSer: 6.215 ± 0.306
4.936LeuThr: 4.936 ± 0.235
5.636LeuVal: 5.636 ± 0.296
0.869LeuTrp: 0.869 ± 0.102
2.764LeuTyr: 2.764 ± 0.208
0.0LeuXaa: 0.0 ± 0.0
Met
2.848MetAla: 2.848 ± 0.179
0.241MetCys: 0.241 ± 0.052
1.617MetAsp: 1.617 ± 0.125
1.472MetGlu: 1.472 ± 0.122
1.086MetPhe: 1.086 ± 0.113
1.617MetGly: 1.617 ± 0.169
0.652MetHis: 0.652 ± 0.085
1.291MetIle: 1.291 ± 0.115
1.521MetLys: 1.521 ± 0.136
2.631MetLeu: 2.631 ± 0.175
0.76MetMet: 0.76 ± 0.096
1.34MetAsn: 1.34 ± 0.123
1.134MetPro: 1.134 ± 0.122
1.34MetGln: 1.34 ± 0.118
1.617MetArg: 1.617 ± 0.154
2.064MetSer: 2.064 ± 0.136
1.653MetThr: 1.653 ± 0.146
1.847MetVal: 1.847 ± 0.163
0.229MetTrp: 0.229 ± 0.052
0.797MetTyr: 0.797 ± 0.088
0.0MetXaa: 0.0 ± 0.0
Asn
4.103AsnAla: 4.103 ± 0.222
0.495AsnCys: 0.495 ± 0.069
2.136AsnAsp: 2.136 ± 0.176
2.281AsnGlu: 2.281 ± 0.148
1.738AsnPhe: 1.738 ± 0.148
3.584AsnGly: 3.584 ± 0.231
0.833AsnHis: 0.833 ± 0.109
2.462AsnIle: 2.462 ± 0.187
2.1AsnLys: 2.1 ± 0.144
3.91AsnLeu: 3.91 ± 0.232
1.134AsnMet: 1.134 ± 0.102
2.233AsnAsn: 2.233 ± 0.192
2.583AsnPro: 2.583 ± 0.204
1.943AsnGln: 1.943 ± 0.173
2.341AsnArg: 2.341 ± 0.17
2.631AsnSer: 2.631 ± 0.172
2.848AsnThr: 2.848 ± 0.199
3.452AsnVal: 3.452 ± 0.206
0.543AsnTrp: 0.543 ± 0.072
1.834AsnTyr: 1.834 ± 0.165
0.0AsnXaa: 0.0 ± 0.0
Pro
4.562ProAla: 4.562 ± 0.506
0.241ProCys: 0.241 ± 0.056
3.102ProAsp: 3.102 ± 0.184
3.572ProGlu: 3.572 ± 0.245
1.883ProPhe: 1.883 ± 0.155
2.546ProGly: 2.546 ± 0.139
0.893ProHis: 0.893 ± 0.115
2.209ProIle: 2.209 ± 0.178
2.619ProLys: 2.619 ± 0.156
3.234ProLeu: 3.234 ± 0.236
0.965ProMet: 0.965 ± 0.1
1.798ProAsn: 1.798 ± 0.125
1.678ProPro: 1.678 ± 0.137
1.847ProGln: 1.847 ± 0.176
1.738ProArg: 1.738 ± 0.158
2.679ProSer: 2.679 ± 0.203
2.607ProThr: 2.607 ± 0.169
3.802ProVal: 3.802 ± 0.401
0.64ProTrp: 0.64 ± 0.09
1.484ProTyr: 1.484 ± 0.138
0.0ProXaa: 0.0 ± 0.0
Gln
4.284GlnAla: 4.284 ± 0.287
0.386GlnCys: 0.386 ± 0.069
2.124GlnAsp: 2.124 ± 0.145
2.559GlnGlu: 2.559 ± 0.184
1.678GlnPhe: 1.678 ± 0.143
3.078GlnGly: 3.078 ± 0.61
0.881GlnHis: 0.881 ± 0.108
2.172GlnIle: 2.172 ± 0.175
1.931GlnLys: 1.931 ± 0.183
3.946GlnLeu: 3.946 ± 0.257
1.134GlnMet: 1.134 ± 0.121
1.726GlnAsn: 1.726 ± 0.137
1.81GlnPro: 1.81 ± 0.258
2.571GlnGln: 2.571 ± 0.309
1.931GlnArg: 1.931 ± 0.173
2.184GlnSer: 2.184 ± 0.174
2.184GlnThr: 2.184 ± 0.201
2.872GlnVal: 2.872 ± 0.213
0.603GlnTrp: 0.603 ± 0.081
1.436GlnTyr: 1.436 ± 0.132
0.0GlnXaa: 0.0 ± 0.0
Arg
4.429ArgAla: 4.429 ± 0.236
0.519ArgCys: 0.519 ± 0.095
3.102ArgAsp: 3.102 ± 0.203
3.162ArgGlu: 3.162 ± 0.208
2.281ArgPhe: 2.281 ± 0.194
2.993ArgGly: 2.993 ± 0.21
1.11ArgHis: 1.11 ± 0.1
3.065ArgIle: 3.065 ± 0.192
2.776ArgLys: 2.776 ± 0.187
4.465ArgLeu: 4.465 ± 0.236
1.786ArgMet: 1.786 ± 0.164
2.74ArgAsn: 2.74 ± 0.181
1.883ArgPro: 1.883 ± 0.137
2.04ArgGln: 2.04 ± 0.155
2.595ArgArg: 2.595 ± 0.181
2.8ArgSer: 2.8 ± 0.202
2.764ArgThr: 2.764 ± 0.194
3.898ArgVal: 3.898 ± 0.21
0.809ArgTrp: 0.809 ± 0.093
2.028ArgTyr: 2.028 ± 0.196
0.0ArgXaa: 0.0 ± 0.0
Ser
5.334SerAla: 5.334 ± 0.316
0.555SerCys: 0.555 ± 0.074
3.524SerAsp: 3.524 ± 0.212
3.331SerGlu: 3.331 ± 0.221
2.74SerPhe: 2.74 ± 0.198
4.26SerGly: 4.26 ± 0.287
1.255SerHis: 1.255 ± 0.139
3.983SerIle: 3.983 ± 0.235
3.078SerLys: 3.078 ± 0.197
5.491SerLeu: 5.491 ± 0.273
2.04SerMet: 2.04 ± 0.132
2.86SerAsn: 2.86 ± 0.187
2.498SerPro: 2.498 ± 0.174
2.172SerGln: 2.172 ± 0.168
3.259SerArg: 3.259 ± 0.176
3.476SerSer: 3.476 ± 0.25
3.778SerThr: 3.778 ± 0.233
4.393SerVal: 4.393 ± 0.224
0.76SerTrp: 0.76 ± 0.091
2.076SerTyr: 2.076 ± 0.16
0.0SerXaa: 0.0 ± 0.0
Thr
4.96ThrAla: 4.96 ± 0.33
0.543ThrCys: 0.543 ± 0.072
3.838ThrAsp: 3.838 ± 0.226
3.584ThrGlu: 3.584 ± 0.207
2.462ThrPhe: 2.462 ± 0.206
4.188ThrGly: 4.188 ± 0.308
1.34ThrHis: 1.34 ± 0.12
3.029ThrIle: 3.029 ± 0.202
3.041ThrLys: 3.041 ± 0.176
5.117ThrLeu: 5.117 ± 0.274
1.303ThrMet: 1.303 ± 0.133
2.559ThrAsn: 2.559 ± 0.166
3.391ThrPro: 3.391 ± 0.213
2.233ThrGln: 2.233 ± 0.183
2.655ThrArg: 2.655 ± 0.176
3.681ThrSer: 3.681 ± 0.235
3.669ThrThr: 3.669 ± 0.293
3.995ThrVal: 3.995 ± 0.236
0.628ThrTrp: 0.628 ± 0.084
2.341ThrTyr: 2.341 ± 0.182
0.0ThrXaa: 0.0 ± 0.0
Val
6.915ValAla: 6.915 ± 0.47
0.7ValCys: 0.7 ± 0.086
4.695ValAsp: 4.695 ± 0.23
4.453ValGlu: 4.453 ± 0.268
3.114ValPhe: 3.114 ± 0.227
4.321ValGly: 4.321 ± 0.26
1.195ValHis: 1.195 ± 0.118
3.765ValIle: 3.765 ± 0.197
3.717ValLys: 3.717 ± 0.229
5.805ValLeu: 5.805 ± 0.218
2.209ValMet: 2.209 ± 0.184
3.065ValAsn: 3.065 ± 0.208
3.319ValPro: 3.319 ± 0.177
3.114ValGln: 3.114 ± 0.201
3.838ValArg: 3.838 ± 0.203
4.357ValSer: 4.357 ± 0.236
4.284ValThr: 4.284 ± 0.246
5.407ValVal: 5.407 ± 0.294
0.797ValTrp: 0.797 ± 0.101
2.209ValTyr: 2.209 ± 0.159
0.0ValXaa: 0.0 ± 0.0
Trp
0.881TrpAla: 0.881 ± 0.101
0.217TrpCys: 0.217 ± 0.056
0.821TrpAsp: 0.821 ± 0.103
0.652TrpGlu: 0.652 ± 0.078
0.398TrpPhe: 0.398 ± 0.068
0.664TrpGly: 0.664 ± 0.085
0.278TrpHis: 0.278 ± 0.063
0.797TrpIle: 0.797 ± 0.112
0.736TrpLys: 0.736 ± 0.094
1.303TrpLeu: 1.303 ± 0.131
0.422TrpMet: 0.422 ± 0.065
0.507TrpAsn: 0.507 ± 0.082
0.471TrpPro: 0.471 ± 0.082
0.422TrpGln: 0.422 ± 0.075
0.676TrpArg: 0.676 ± 0.097
0.748TrpSer: 0.748 ± 0.089
0.7TrpThr: 0.7 ± 0.085
0.797TrpVal: 0.797 ± 0.108
0.169TrpTrp: 0.169 ± 0.05
0.543TrpTyr: 0.543 ± 0.083
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.776TyrAla: 2.776 ± 0.18
0.507TyrCys: 0.507 ± 0.082
2.607TyrAsp: 2.607 ± 0.202
2.076TyrGlu: 2.076 ± 0.155
1.726TyrPhe: 1.726 ± 0.164
2.378TyrGly: 2.378 ± 0.173
0.869TyrHis: 0.869 ± 0.106
1.714TyrIle: 1.714 ± 0.161
1.641TyrLys: 1.641 ± 0.119
3.126TyrLeu: 3.126 ± 0.211
0.821TyrMet: 0.821 ± 0.106
1.569TyrAsn: 1.569 ± 0.122
1.267TyrPro: 1.267 ± 0.132
1.448TyrGln: 1.448 ± 0.152
2.148TyrArg: 2.148 ± 0.141
2.245TyrSer: 2.245 ± 0.19
1.955TyrThr: 1.955 ± 0.155
2.764TyrVal: 2.764 ± 0.179
0.471TyrTrp: 0.471 ± 0.081
1.593TyrTyr: 1.593 ± 0.15
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 287 proteins (82860 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski