Synechococcus virus S-PRM1
Average proteome isoelectric point is 5.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346FKD2|A0A346FKD2_9CAUD Uncharacterized protein OS=Synechococcus virus S-PRM1 OX=2100130 PE=4 SV=1
MM1 pKa = 7.42 LAISLVFGALFAVGGTIVGFISGWFANEE29 pKa = 4.01 KK30 pKa = 8.48 YY31 pKa = 10.43 SEE33 pKa = 4.16 YY34 pKa = 11.25 VEE36 pKa = 5.5 LKK38 pKa = 7.75 TAQIATHH45 pKa = 6.65 PEE47 pKa = 3.9 MYY49 pKa = 9.25 DD50 pKa = 3.27 TEE52 pKa = 4.92 GNLITSQLTALRR64 pKa = 11.84 VVLDD68 pKa = 4.46 DD69 pKa = 3.51 QDD71 pKa = 4.53 FYY73 pKa = 11.97 YY74 pKa = 10.92 DD75 pKa = 5.49 DD76 pKa = 4.13 EE77 pKa = 4.75 DD78 pKa = 3.64
Molecular weight: 8.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.859
IPC_protein 3.757
Toseland 3.567
ProMoST 3.834
Dawson 3.745
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.884
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A346FKM1|A0A346FKM1_9CAUD Major capsid protein OS=Synechococcus virus S-PRM1 OX=2100130 PE=4 SV=1
MM1 pKa = 7.13 KK2 pKa = 9.82 TFNQFRR8 pKa = 11.84 EE9 pKa = 4.25 EE10 pKa = 4.58 CGLEE14 pKa = 4.08 EE15 pKa = 4.12 NWLKK19 pKa = 11.2 SLFGGSKK26 pKa = 9.82 PAQTKK31 pKa = 9.23 PPKK34 pKa = 9.59 PANQNVLAYY43 pKa = 9.77 KK44 pKa = 10.29 NYY46 pKa = 10.03 QPGVLDD52 pKa = 3.22 KK53 pKa = 8.72 TTNKK57 pKa = 7.49 FTPRR61 pKa = 11.84 SFTKK65 pKa = 10.42 PEE67 pKa = 3.6 QQRR70 pKa = 11.84 YY71 pKa = 6.25 GWKK74 pKa = 9.59 PVNTSSYY81 pKa = 10.83 GPGDD85 pKa = 3.73 TTSQGYY91 pKa = 7.59 NTGADD96 pKa = 3.09 RR97 pKa = 11.84 VQRR100 pKa = 11.84 TADD103 pKa = 3.43 GTPFTGSTRR112 pKa = 11.84 GVAVPYY118 pKa = 9.8 KK119 pKa = 10.01 YY120 pKa = 10.14 KK121 pKa = 10.91 ANEE124 pKa = 4.09 VPKK127 pKa = 9.22 GTWAGTPSQDD137 pKa = 4.54 FGTNLQFTQKK147 pKa = 10.76 PMGTNTRR154 pKa = 11.84 VTNATVRR161 pKa = 11.84 DD162 pKa = 3.8 TGNFGPAGEE171 pKa = 4.43 VNRR174 pKa = 11.84 NTSYY178 pKa = 11.88 DD179 pKa = 3.52 LMRR182 pKa = 11.84 QTARR186 pKa = 11.84 DD187 pKa = 3.53 VTGNPNLTPTQYY199 pKa = 10.94 GKK201 pKa = 10.44 RR202 pKa = 11.84 KK203 pKa = 9.53 VYY205 pKa = 10.38 VRR207 pKa = 11.84 TAPRR211 pKa = 11.84 RR212 pKa = 11.84 SVTTEE217 pKa = 3.35 RR218 pKa = 4.0
Molecular weight: 24.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.22
IPC2_protein 9.575
IPC_protein 9.75
Toseland 10.204
ProMoST 9.94
Dawson 10.394
Bjellqvist 10.058
Wikipedia 10.57
Rodwell 10.818
Grimsley 10.467
Solomon 10.423
Lehninger 10.379
Nozaki 10.175
DTASelect 10.058
Thurlkill 10.248
EMBOSS 10.599
Sillero 10.306
Patrickios 10.452
IPC_peptide 10.423
IPC2_peptide 8.697
IPC2.peptide.svr19 8.616
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
190
0
190
44622
54
2133
234.9
26.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.266 ± 0.212
1.038 ± 0.082
6.633 ± 0.129
6.752 ± 0.268
4.31 ± 0.122
7.505 ± 0.416
1.773 ± 0.106
6.174 ± 0.158
6.49 ± 0.332
7.496 ± 0.205
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.154
5.504 ± 0.227
4.029 ± 0.144
3.628 ± 0.121
4.498 ± 0.153
6.414 ± 0.201
6.822 ± 0.494
6.925 ± 0.178
1.259 ± 0.11
4.229 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here