Candidatus Xenolissoclinum pacificiensis L6
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 956 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W2V1W7|W2V1W7_9RICK Uncharacterized protein OS=Candidatus Xenolissoclinum pacificiensis L6 OX=1401685 GN=P857_358 PE=4 SV=1
MM1 pKa = 7.66 IEE3 pKa = 3.72 ANYY6 pKa = 10.16 FFTQFTNFLDD16 pKa = 4.21 EE17 pKa = 5.3 DD18 pKa = 4.47 PEE20 pKa = 4.51 TMKK23 pKa = 10.34 IEE25 pKa = 3.96 YY26 pKa = 10.25 SILDD30 pKa = 3.39 NVTNYY35 pKa = 8.96 FWSFVAKK42 pKa = 10.17 VDD44 pKa = 4.51 PEE46 pKa = 4.14 KK47 pKa = 10.94 CSQDD51 pKa = 3.5 KK52 pKa = 11.18 LNEE55 pKa = 3.89 SCAFFPIWLMSDD67 pKa = 3.58 DD68 pKa = 4.25 FFF70 pKa = 6.98
Molecular weight: 8.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.859
IPC_protein 3.757
Toseland 3.567
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|W2UZV6|W2UZV6_9RICK Phosphatidylserine decarboxylase proenzyme OS=Candidatus Xenolissoclinum pacificiensis L6 OX=1401685 GN=psd PE=4 SV=1
MM1 pKa = 7.61 GKK3 pKa = 10.25 LKK5 pKa = 9.96 TKK7 pKa = 10.45 SGLKK11 pKa = 10.34 GRR13 pKa = 11.84 FKK15 pKa = 10.24 VTATGKK21 pKa = 10.57 VMATQSGKK29 pKa = 9.71 RR30 pKa = 11.84 HH31 pKa = 5.65 NLRR34 pKa = 11.84 KK35 pKa = 9.72 RR36 pKa = 11.84 SPRR39 pKa = 11.84 SSRR42 pKa = 11.84 GQKK45 pKa = 10.13 GFVRR49 pKa = 11.84 LGAHH53 pKa = 5.62 GTTLIKK59 pKa = 10.48 YY60 pKa = 8.22 LKK62 pKa = 9.92 NNRR65 pKa = 11.84 GG66 pKa = 3.21
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.427
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.149
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.956
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
956
0
956
269146
37
3035
281.5
32.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.597 ± 0.068
1.466 ± 0.034
5.741 ± 0.07
5.491 ± 0.07
4.608 ± 0.065
5.117 ± 0.097
2.321 ± 0.045
9.774 ± 0.104
7.105 ± 0.084
9.423 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.698 ± 0.042
6.264 ± 0.085
2.999 ± 0.045
3.652 ± 0.073
3.845 ± 0.059
8.087 ± 0.07
5.043 ± 0.076
6.697 ± 0.086
0.626 ± 0.023
4.446 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here