Amino acid dipepetide frequency for Streptomyces rubidus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	25.185AlaAla: 25.185 ± 0.171	1.102AlaCys: 1.102 ± 0.022	8.763AlaAsp: 8.763 ± 0.076	8.038AlaGlu: 8.038 ± 0.086	3.636AlaPhe: 3.636 ± 0.037	14.973AlaGly: 14.973 ± 0.098	3.065AlaHis: 3.065 ± 0.037	3.37AlaIle: 3.37 ± 0.04	2.494AlaLys: 2.494 ± 0.04	14.617AlaLeu: 14.617 ± 0.097	2.543AlaMet: 2.543 ± 0.031	2.106AlaAsn: 2.106 ± 0.038	7.992AlaPro: 7.992 ± 0.096	4.027AlaGln: 4.027 ± 0.049	10.4AlaArg: 10.4 ± 0.087	6.612AlaSer: 6.612 ± 0.06	7.867AlaThr: 7.867 ± 0.077	12.882AlaVal: 12.882 ± 0.091	1.998AlaTrp: 1.998 ± 0.026	2.857AlaTyr: 2.857 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.046CysAla: 1.046 ± 0.026	0.095CysCys: 0.095 ± 0.007	0.439CysAsp: 0.439 ± 0.014	0.341CysGlu: 0.341 ± 0.012	0.208CysPhe: 0.208 ± 0.009	0.94CysGly: 0.94 ± 0.024	0.195CysHis: 0.195 ± 0.008	0.147CysIle: 0.147 ± 0.007	0.095CysLys: 0.095 ± 0.007	0.726CysLeu: 0.726 ± 0.017	0.117CysMet: 0.117 ± 0.007	0.164CysAsn: 0.164 ± 0.01	0.452CysPro: 0.452 ± 0.014	0.174CysGln: 0.174 ± 0.009	0.543CysArg: 0.543 ± 0.016	0.442CysSer: 0.442 ± 0.014	0.545CysThr: 0.545 ± 0.016	0.608CysVal: 0.608 ± 0.016	0.14CysTrp: 0.14 ± 0.007	0.144CysTyr: 0.144 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	8.28AspAla: 8.28 ± 0.067	0.424AspCys: 0.424 ± 0.013	3.549AspAsp: 3.549 ± 0.039	3.31AspGlu: 3.31 ± 0.042	1.561AspPhe: 1.561 ± 0.027	6.889AspGly: 6.889 ± 0.065	1.47AspHis: 1.47 ± 0.025	1.682AspIle: 1.682 ± 0.031	0.942AspLys: 0.942 ± 0.021	6.221AspLeu: 6.221 ± 0.061	0.776AspMet: 0.776 ± 0.017	0.984AspAsn: 0.984 ± 0.022	4.63AspPro: 4.63 ± 0.049	1.619AspGln: 1.619 ± 0.025	4.74AspArg: 4.74 ± 0.052	2.51AspSer: 2.51 ± 0.034	3.36AspThr: 3.36 ± 0.04	4.895AspVal: 4.895 ± 0.051	1.002AspTrp: 1.002 ± 0.022	1.144AspTyr: 1.144 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.547GluAla: 6.547 ± 0.074	0.326GluCys: 0.326 ± 0.011	2.531GluAsp: 2.531 ± 0.034	2.816GluGlu: 2.816 ± 0.046	1.327GluPhe: 1.327 ± 0.024	3.739GluGly: 3.739 ± 0.049	1.391GluHis: 1.391 ± 0.025	1.889GluIle: 1.889 ± 0.029	0.945GluLys: 0.945 ± 0.023	5.788GluLeu: 5.788 ± 0.063	0.759GluMet: 0.759 ± 0.018	0.854GluAsn: 0.854 ± 0.018	3.118GluPro: 3.118 ± 0.043	2.037GluGln: 2.037 ± 0.028	4.735GluArg: 4.735 ± 0.053	2.266GluSer: 2.266 ± 0.03	2.522GluThr: 2.522 ± 0.039	3.968GluVal: 3.968 ± 0.049	0.665GluTrp: 0.665 ± 0.016	0.912GluTyr: 0.912 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.741PheAla: 3.741 ± 0.042	0.258PheCys: 0.258 ± 0.011	1.907PheAsp: 1.907 ± 0.029	1.192PheGlu: 1.192 ± 0.02	0.813PhePhe: 0.813 ± 0.021	2.989PheGly: 2.989 ± 0.037	0.622PheHis: 0.622 ± 0.016	0.584PheIle: 0.584 ± 0.015	0.405PheLys: 0.405 ± 0.013	2.435PheLeu: 2.435 ± 0.038	0.376PheMet: 0.376 ± 0.015	0.521PheAsn: 0.521 ± 0.014	1.359PhePro: 1.359 ± 0.022	0.695PheGln: 0.695 ± 0.017	1.755PheArg: 1.755 ± 0.027	1.453PheSer: 1.453 ± 0.029	2.191PheThr: 2.191 ± 0.032	2.084PheVal: 2.084 ± 0.036	0.412PheTrp: 0.412 ± 0.012	0.567PheTyr: 0.567 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	12.159GlyAla: 12.159 ± 0.094	0.818GlyCys: 0.818 ± 0.019	5.621GlyAsp: 5.621 ± 0.056	4.578GlyGlu: 4.578 ± 0.053	2.829GlyPhe: 2.829 ± 0.034	10.115GlyGly: 10.115 ± 0.09	2.547GlyHis: 2.547 ± 0.035	3.186GlyIle: 3.186 ± 0.042	2.056GlyLys: 2.056 ± 0.033	9.361GlyLeu: 9.361 ± 0.062	1.936GlyMet: 1.936 ± 0.028	1.926GlyAsn: 1.926 ± 0.037	5.853GlyPro: 5.853 ± 0.07	2.934GlyGln: 2.934 ± 0.037	8.036GlyArg: 8.036 ± 0.065	6.04GlySer: 6.04 ± 0.064	7.667GlyThr: 7.667 ± 0.071	7.543GlyVal: 7.543 ± 0.059	1.874GlyTrp: 1.874 ± 0.036	2.48GlyTyr: 2.48 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	3.061HisAla: 3.061 ± 0.041	0.18HisCys: 0.18 ± 0.008	1.456HisAsp: 1.456 ± 0.026	1.097HisGlu: 1.097 ± 0.022	0.65HisPhe: 0.65 ± 0.015	2.613HisGly: 2.613 ± 0.034	0.726HisHis: 0.726 ± 0.02	0.636HisIle: 0.636 ± 0.018	0.302HisLys: 0.302 ± 0.011	2.463HisLeu: 2.463 ± 0.039	0.32HisMet: 0.32 ± 0.011	0.369HisAsn: 0.369 ± 0.012	1.827HisPro: 1.827 ± 0.029	0.694HisGln: 0.694 ± 0.019	2.082HisArg: 2.082 ± 0.029	1.05HisSer: 1.05 ± 0.02	1.429HisThr: 1.429 ± 0.026	1.778HisVal: 1.778 ± 0.023	0.41HisTrp: 0.41 ± 0.013	0.491HisTyr: 0.491 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.532IleAla: 4.532 ± 0.044	0.239IleCys: 0.239 ± 0.01	2.112IleAsp: 2.112 ± 0.032	1.607IleGlu: 1.607 ± 0.026	0.638IlePhe: 0.638 ± 0.017	3.369IleGly: 3.369 ± 0.04	0.57IleHis: 0.57 ± 0.015	0.752IleIle: 0.752 ± 0.022	0.583IleLys: 0.583 ± 0.017	2.138IleLeu: 2.138 ± 0.038	0.386IleMet: 0.386 ± 0.013	0.631IleAsn: 0.631 ± 0.018	1.61IlePro: 1.61 ± 0.029	0.657IleGln: 0.657 ± 0.015	2.014IleArg: 2.014 ± 0.027	1.597IleSer: 1.597 ± 0.027	2.131IleThr: 2.131 ± 0.031	2.485IleVal: 2.485 ± 0.034	0.331IleTrp: 0.331 ± 0.011	0.51IleTyr: 0.51 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.536LysAla: 2.536 ± 0.041	0.112LysCys: 0.112 ± 0.008	1.137LysAsp: 1.137 ± 0.025	0.847LysGlu: 0.847 ± 0.021	0.378LysPhe: 0.378 ± 0.012	1.56LysGly: 1.56 ± 0.027	0.371LysHis: 0.371 ± 0.013	0.668LysIle: 0.668 ± 0.017	0.544LysLys: 0.544 ± 0.02	1.605LysLeu: 1.605 ± 0.026	0.302LysMet: 0.302 ± 0.011	0.423LysAsn: 0.423 ± 0.014	1.127LysPro: 1.127 ± 0.026	0.598LysGln: 0.598 ± 0.016	1.114LysArg: 1.114 ± 0.025	0.971LysSer: 0.971 ± 0.022	1.058LysThr: 1.058 ± 0.025	1.603LysVal: 1.603 ± 0.027	0.229LysTrp: 0.229 ± 0.01	0.377LysTyr: 0.377 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	15.253LeuAla: 15.253 ± 0.108	0.766LeuCys: 0.766 ± 0.018	6.603LeuAsp: 6.603 ± 0.055	4.06LeuGlu: 4.06 ± 0.05	2.449LeuPhe: 2.449 ± 0.035	9.079LeuGly: 9.079 ± 0.071	2.392LeuHis: 2.392 ± 0.034	2.881LeuIle: 2.881 ± 0.043	1.604LeuLys: 1.604 ± 0.03	10.725LeuLeu: 10.725 ± 0.095	1.546LeuMet: 1.546 ± 0.026	1.627LeuAsn: 1.627 ± 0.028	6.61LeuPro: 6.61 ± 0.06	2.308LeuGln: 2.308 ± 0.033	8.282LeuArg: 8.282 ± 0.071	5.273LeuSer: 5.273 ± 0.043	6.765LeuThr: 6.765 ± 0.048	8.519LeuVal: 8.519 ± 0.064	1.302LeuTrp: 1.302 ± 0.027	1.824LeuTyr: 1.824 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.214MetAla: 2.214 ± 0.032	0.127MetCys: 0.127 ± 0.006	0.903MetAsp: 0.903 ± 0.02	0.65MetGlu: 0.65 ± 0.016	0.433MetPhe: 0.433 ± 0.015	1.259MetGly: 1.259 ± 0.023	0.36MetHis: 0.36 ± 0.011	0.575MetIle: 0.575 ± 0.015	0.351MetLys: 0.351 ± 0.011	1.602MetLeu: 1.602 ± 0.026	0.284MetMet: 0.284 ± 0.012	0.436MetAsn: 0.436 ± 0.012	1.144MetPro: 1.144 ± 0.021	0.439MetGln: 0.439 ± 0.014	1.392MetArg: 1.392 ± 0.023	1.263MetSer: 1.263 ± 0.021	1.518MetThr: 1.518 ± 0.027	1.242MetVal: 1.242 ± 0.023	0.209MetTrp: 0.209 ± 0.01	0.312MetTyr: 0.312 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.376AsnAla: 2.376 ± 0.032	0.145AsnCys: 0.145 ± 0.008	0.993AsnAsp: 0.993 ± 0.02	0.713AsnGlu: 0.713 ± 0.015	0.448AsnPhe: 0.448 ± 0.015	2.193AsnGly: 2.193 ± 0.04	0.391AsnHis: 0.391 ± 0.013	0.561AsnIle: 0.561 ± 0.016	0.338AsnLys: 0.338 ± 0.012	1.631AsnLeu: 1.631 ± 0.03	0.269AsnMet: 0.269 ± 0.011	0.445AsnAsn: 0.445 ± 0.015	1.383AsnPro: 1.383 ± 0.021	0.532AsnGln: 0.532 ± 0.016	1.214AsnArg: 1.214 ± 0.022	1.026AsnSer: 1.026 ± 0.027	1.181AsnThr: 1.181 ± 0.029	1.422AsnVal: 1.422 ± 0.024	0.299AsnTrp: 0.299 ± 0.011	0.399AsnTyr: 0.399 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	10.216ProAla: 10.216 ± 0.096	0.347ProCys: 0.347 ± 0.014	4.448ProAsp: 4.448 ± 0.046	3.778ProGlu: 3.778 ± 0.053	1.514ProPhe: 1.514 ± 0.024	7.468ProGly: 7.468 ± 0.076	1.538ProHis: 1.538 ± 0.028	1.246ProIle: 1.246 ± 0.023	1.01ProLys: 1.01 ± 0.022	5.386ProLeu: 5.386 ± 0.05	0.988ProMet: 0.988 ± 0.019	0.947ProAsn: 0.947 ± 0.023	4.039ProPro: 4.039 ± 0.074	1.954ProGln: 1.954 ± 0.037	4.179ProArg: 4.179 ± 0.053	3.545ProSer: 3.545 ± 0.047	3.237ProThr: 3.237 ± 0.044	5.371ProVal: 5.371 ± 0.052	0.914ProTrp: 0.914 ± 0.022	1.406ProTyr: 1.406 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	4.106GlnAla: 4.106 ± 0.048	0.172GlnCys: 0.172 ± 0.008	1.548GlnAsp: 1.548 ± 0.023	1.342GlnGlu: 1.342 ± 0.026	0.721GlnPhe: 0.721 ± 0.021	2.495GlnGly: 2.495 ± 0.035	0.706GlnHis: 0.706 ± 0.015	1.074GlnIle: 1.074 ± 0.02	0.543GlnLys: 0.543 ± 0.016	2.979GlnLeu: 2.979 ± 0.038	0.494GlnMet: 0.494 ± 0.013	0.571GlnAsn: 0.571 ± 0.017	1.864GlnPro: 1.864 ± 0.029	1.363GlnGln: 1.363 ± 0.032	2.315GlnArg: 2.315 ± 0.033	1.418GlnSer: 1.418 ± 0.028	1.444GlnThr: 1.444 ± 0.026	2.592GlnVal: 2.592 ± 0.034	0.492GlnTrp: 0.492 ± 0.014	0.636GlnTyr: 0.636 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.966ArgAla: 9.966 ± 0.084	0.563ArgCys: 0.563 ± 0.016	4.213ArgAsp: 4.213 ± 0.043	4.098ArgGlu: 4.098 ± 0.049	2.116ArgPhe: 2.116 ± 0.029	5.899ArgGly: 5.899 ± 0.054	2.112ArgHis: 2.112 ± 0.03	3.028ArgIle: 3.028 ± 0.035	1.303ArgLys: 1.303 ± 0.025	8.493ArgLeu: 8.493 ± 0.071	1.724ArgMet: 1.724 ± 0.026	1.289ArgAsn: 1.289 ± 0.022	5.055ArgPro: 5.055 ± 0.054	2.384ArgGln: 2.384 ± 0.033	7.607ArgArg: 7.607 ± 0.078	3.856ArgSer: 3.856 ± 0.039	5.369ArgThr: 5.369 ± 0.05	5.645ArgVal: 5.645 ± 0.059	1.377ArgTrp: 1.377 ± 0.025	1.74ArgTyr: 1.74 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.526SerAla: 7.526 ± 0.068	0.411SerCys: 0.411 ± 0.012	2.804SerAsp: 2.804 ± 0.039	2.067SerGlu: 2.067 ± 0.028	1.476SerPhe: 1.476 ± 0.026	6.75SerGly: 6.75 ± 0.071	1.076SerHis: 1.076 ± 0.023	1.342SerIle: 1.342 ± 0.028	0.872SerLys: 0.872 ± 0.021	4.744SerLeu: 4.744 ± 0.044	1.044SerMet: 1.044 ± 0.021	0.925SerAsn: 0.925 ± 0.023	3.4SerPro: 3.4 ± 0.045	1.367SerGln: 1.367 ± 0.024	3.678SerArg: 3.678 ± 0.04	3.27SerSer: 3.27 ± 0.051	3.446SerThr: 3.446 ± 0.052	4.323SerVal: 4.323 ± 0.042	0.967SerTrp: 0.967 ± 0.021	1.269SerTyr: 1.269 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	10.126ThrAla: 10.126 ± 0.084	0.442ThrCys: 0.442 ± 0.012	3.667ThrAsp: 3.667 ± 0.046	2.741ThrGlu: 2.741 ± 0.03	1.711ThrPhe: 1.711 ± 0.03	7.442ThrGly: 7.442 ± 0.069	1.251ThrHis: 1.251 ± 0.026	1.642ThrIle: 1.642 ± 0.027	1.021ThrLys: 1.021 ± 0.022	5.783ThrLeu: 5.783 ± 0.055	0.933ThrMet: 0.933 ± 0.019	1.08ThrAsn: 1.08 ± 0.025	4.487ThrPro: 4.487 ± 0.053	1.473ThrGln: 1.473 ± 0.028	3.875ThrArg: 3.875 ± 0.043	3.684ThrSer: 3.684 ± 0.061	4.432ThrThr: 4.432 ± 0.087	6.445ThrVal: 6.445 ± 0.066	1.003ThrTrp: 1.003 ± 0.021	1.504ThrTyr: 1.504 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	11.094ValAla: 11.094 ± 0.082	0.707ValCys: 0.707 ± 0.017	4.88ValAsp: 4.88 ± 0.042	4.174ValGlu: 4.174 ± 0.05	2.324ValPhe: 2.324 ± 0.036	6.401ValGly: 6.401 ± 0.059	2.026ValHis: 2.026 ± 0.028	2.648ValIle: 2.648 ± 0.035	1.496ValLys: 1.496 ± 0.029	9.416ValLeu: 9.416 ± 0.072	1.368ValMet: 1.368 ± 0.024	1.705ValAsn: 1.705 ± 0.03	5.6ValPro: 5.6 ± 0.049	2.205ValGln: 2.205 ± 0.03	6.789ValArg: 6.789 ± 0.064	4.389ValSer: 4.389 ± 0.044	6.018ValThr: 6.018 ± 0.067	7.927ValVal: 7.927 ± 0.074	1.151ValTrp: 1.151 ± 0.021	1.636ValTyr: 1.636 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.712TrpAla: 1.712 ± 0.027	0.15TrpCys: 0.15 ± 0.007	0.84TrpAsp: 0.84 ± 0.02	0.663TrpGlu: 0.663 ± 0.015	0.488TrpPhe: 0.488 ± 0.016	1.086TrpGly: 1.086 ± 0.026	0.413TrpHis: 0.413 ± 0.012	0.554TrpIle: 0.554 ± 0.016	0.324TrpLys: 0.324 ± 0.011	1.697TrpLeu: 1.697 ± 0.027	0.297TrpMet: 0.297 ± 0.011	0.483TrpAsn: 0.483 ± 0.017	0.843TrpPro: 0.843 ± 0.02	0.682TrpGln: 0.682 ± 0.016	1.264TrpArg: 1.264 ± 0.021	1.052TrpSer: 1.052 ± 0.024	1.19TrpThr: 1.19 ± 0.026	0.984TrpVal: 0.984 ± 0.022	0.347TrpTrp: 0.347 ± 0.014	0.397TrpTyr: 0.397 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.853TyrAla: 2.853 ± 0.034	0.176TyrCys: 0.176 ± 0.008	1.572TyrAsp: 1.572 ± 0.045	1.026TyrGlu: 1.026 ± 0.022	0.637TyrPhe: 0.637 ± 0.017	2.3TyrGly: 2.3 ± 0.034	0.414TyrHis: 0.414 ± 0.012	0.428TyrIle: 0.428 ± 0.013	0.331TyrLys: 0.331 ± 0.011	2.059TyrLeu: 2.059 ± 0.029	0.252TyrMet: 0.252 ± 0.01	0.474TyrAsn: 0.474 ± 0.019	1.136TyrPro: 1.136 ± 0.026	0.73TyrGln: 0.73 ± 0.019	1.822TyrArg: 1.822 ± 0.03	1.039TyrSer: 1.039 ± 0.024	1.372TyrThr: 1.372 ± 0.035	1.64TyrVal: 1.64 ± 0.025	0.343TyrTrp: 0.343 ± 0.013	0.481TyrTyr: 0.481 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 7682 proteins (2598787 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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