Maize-associated totivirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A193GXV6|A0A193GXV6_9VIRU Coat protein OS=Maize-associated totivirus 2 OX=1874267 PE=4 SV=2
MM1 pKa = 7.59DD2 pKa = 3.86TFVRR6 pKa = 11.84HH7 pKa = 5.63FFKK10 pKa = 10.78EE11 pKa = 4.03VTTPGVPFVLDD22 pKa = 4.21DD23 pKa = 4.33ACHH26 pKa = 5.44TWTYY30 pKa = 10.17FQGFKK35 pKa = 10.27TVTTGDD41 pKa = 3.41ATKK44 pKa = 10.77SSARR48 pKa = 11.84DD49 pKa = 3.17IYY51 pKa = 11.26QAMTPLGMLDD61 pKa = 3.34AAFEE65 pKa = 4.8SMGSNYY71 pKa = 10.7DD72 pKa = 3.6GLTKK76 pKa = 10.65NYY78 pKa = 7.33LTPEE82 pKa = 3.95GAINFSNIHH91 pKa = 6.26DD92 pKa = 4.01TLKK95 pKa = 10.89QSGGMNNQDD104 pKa = 3.96LSTFMAFCQTISWHH118 pKa = 6.73DD119 pKa = 3.44NHH121 pKa = 5.78VTLLFNMLRR130 pKa = 11.84YY131 pKa = 9.76VILMKK136 pKa = 10.81FKK138 pKa = 10.51EE139 pKa = 4.58CKK141 pKa = 9.74QNLNGALGRR150 pKa = 11.84FQEE153 pKa = 5.08DD154 pKa = 3.44FLEE157 pKa = 5.15INMDD161 pKa = 3.75QYY163 pKa = 11.43WPDD166 pKa = 3.29SYY168 pKa = 11.53PRR170 pKa = 11.84DD171 pKa = 3.73CAFDD175 pKa = 3.44SWPVDD180 pKa = 3.5VTEE183 pKa = 5.48DD184 pKa = 3.26GAIALEE190 pKa = 4.53HH191 pKa = 6.41LTTDD195 pKa = 3.15QTRR198 pKa = 11.84NIGPAIDD205 pKa = 4.35LRR207 pKa = 11.84GLTSAEE213 pKa = 4.19SIFVIFMLHH222 pKa = 4.92KK223 pKa = 7.64WTRR226 pKa = 11.84SSRR229 pKa = 11.84FKK231 pKa = 11.11LDD233 pKa = 2.99VSTKK237 pKa = 10.71LLTNTLQFRR246 pKa = 11.84GVDD249 pKa = 3.35VSALYY254 pKa = 10.42DD255 pKa = 3.9SYY257 pKa = 11.06WKK259 pKa = 10.73EE260 pKa = 3.97EE261 pKa = 4.11TVMPALLSSNAAWHH275 pKa = 6.52ALAKK279 pKa = 10.59YY280 pKa = 7.53VTQNRR285 pKa = 11.84LFDD288 pKa = 3.71QFSSALYY295 pKa = 10.36LVTALTYY302 pKa = 10.44QYY304 pKa = 11.16VPEE307 pKa = 4.19TAEE310 pKa = 4.24GVSWLSLNWRR320 pKa = 11.84VVLPEE325 pKa = 3.81FTSIRR330 pKa = 11.84GKK332 pKa = 10.35FAFFNTGDD340 pKa = 3.66AAFLSQRR347 pKa = 11.84SLDD350 pKa = 3.2EE351 pKa = 3.74WEE353 pKa = 4.37YY354 pKa = 11.01IGGKK358 pKa = 7.04MEE360 pKa = 5.39KK361 pKa = 9.65INLMSLTFCQAFYY374 pKa = 11.16AGIALRR380 pKa = 11.84TTRR383 pKa = 11.84RR384 pKa = 11.84GLEE387 pKa = 3.85MMPADD392 pKa = 4.17IYY394 pKa = 11.06GTDD397 pKa = 3.36AEE399 pKa = 4.62FWNAEE404 pKa = 4.15NFVSAAVAEE413 pKa = 4.58FARR416 pKa = 11.84IKK418 pKa = 10.79VPMSGTPGLYY428 pKa = 8.37ITCDD432 pKa = 3.2EE433 pKa = 4.64RR434 pKa = 11.84LDD436 pKa = 3.58IVNEE440 pKa = 3.79HH441 pKa = 6.45RR442 pKa = 11.84FVMTNNPNRR451 pKa = 11.84RR452 pKa = 11.84DD453 pKa = 3.65LPPTHH458 pKa = 7.23TYY460 pKa = 11.41GEE462 pKa = 4.48TQTNEE467 pKa = 4.06TVVDD471 pKa = 5.03DD472 pKa = 4.87IEE474 pKa = 5.74LDD476 pKa = 3.65PTTLPDD482 pKa = 4.64RR483 pKa = 11.84DD484 pKa = 4.08PEE486 pKa = 4.13KK487 pKa = 10.49QALINGMRR495 pKa = 11.84VAKK498 pKa = 10.46VGAEE502 pKa = 3.92AMRR505 pKa = 11.84AVDD508 pKa = 4.51PEE510 pKa = 4.27CSLPAGFEE518 pKa = 3.62LALRR522 pKa = 11.84DD523 pKa = 3.25RR524 pKa = 11.84VAARR528 pKa = 11.84RR529 pKa = 11.84PRR531 pKa = 11.84VRR533 pKa = 11.84QAARR537 pKa = 11.84VTVACPLLPFPGVPTLLLPTQCTPYY562 pKa = 10.35PIPFEE567 pKa = 4.04LAGKK571 pKa = 10.23FDD573 pKa = 5.08DD574 pKa = 3.74NLGRR578 pKa = 11.84FDD580 pKa = 4.74RR581 pKa = 11.84RR582 pKa = 11.84GTRR585 pKa = 11.84LKK587 pKa = 10.13IDD589 pKa = 3.58AAWRR593 pKa = 11.84VMNFLQDD600 pKa = 3.54QSYY603 pKa = 8.1TWEE606 pKa = 3.98CEE608 pKa = 3.82YY609 pKa = 10.85RR610 pKa = 11.84GTRR613 pKa = 11.84IGQQEE618 pKa = 4.44YY619 pKa = 10.05VRR621 pKa = 11.84GRR623 pKa = 11.84YY624 pKa = 9.93SNMIWPVLYY633 pKa = 10.44QPEE636 pKa = 4.66DD637 pKa = 3.51NDD639 pKa = 3.26EE640 pKa = 4.28DD641 pKa = 4.11VVLRR645 pKa = 11.84RR646 pKa = 11.84ITEE649 pKa = 3.81VGLPDD654 pKa = 3.66VPLPPMHH661 pKa = 6.93NEE663 pKa = 3.74FFKK666 pKa = 11.02GRR668 pKa = 11.84SIEE671 pKa = 3.95YY672 pKa = 9.04KK673 pKa = 8.21YY674 pKa = 10.98QSIRR678 pKa = 11.84RR679 pKa = 11.84GLATRR684 pKa = 11.84FRR686 pKa = 11.84GAKK689 pKa = 9.23IDD691 pKa = 3.37ISSYY695 pKa = 8.68GAHH698 pKa = 5.7VPVLKK703 pKa = 10.57KK704 pKa = 10.46ISTVTYY710 pKa = 10.22AVNEE714 pKa = 4.09GLRR717 pKa = 11.84TVRR720 pKa = 11.84GFIEE724 pKa = 4.44RR725 pKa = 11.84AAQDD729 pKa = 3.36FRR731 pKa = 11.84LAPVAGPGVIPPVEE745 pKa = 3.96QAPDD749 pKa = 3.14ASAATGGQEE758 pKa = 4.33TGGTSAA764 pKa = 5.04

Molecular weight:
86.21 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A193GXK6|A0A193GXK6_9VIRU RNA-directed RNA polymerase OS=Maize-associated totivirus 2 OX=1874267 PE=3 SV=2
MM1 pKa = 7.59LNATPGGDD9 pKa = 3.23GPYY12 pKa = 9.95IKK14 pKa = 10.19RR15 pKa = 11.84KK16 pKa = 9.8EE17 pKa = 4.04PLTLNNIPVWVNIRR31 pKa = 11.84HH32 pKa = 5.18NHH34 pKa = 4.33LTAVDD39 pKa = 3.51RR40 pKa = 11.84HH41 pKa = 5.28DD42 pKa = 3.56AKK44 pKa = 11.12YY45 pKa = 10.76VLGFILPGQSFEE57 pKa = 4.06VGGFGRR63 pKa = 11.84YY64 pKa = 8.25LVGKK68 pKa = 7.45TCVAAVAIPCDD79 pKa = 4.17GMWLLYY85 pKa = 10.92VSVTTPANILPPIVKK100 pKa = 8.62RR101 pKa = 11.84TLSAMFSSVDD111 pKa = 3.01GYY113 pKa = 11.28YY114 pKa = 10.48FHH116 pKa = 8.03DD117 pKa = 4.89ADD119 pKa = 3.74TTKK122 pKa = 10.91YY123 pKa = 10.19LRR125 pKa = 11.84KK126 pKa = 7.98TFDD129 pKa = 3.23VEE131 pKa = 4.18RR132 pKa = 11.84SAIAHH137 pKa = 6.09AAKK140 pKa = 9.96PIPPRR145 pKa = 11.84EE146 pKa = 4.28GEE148 pKa = 3.98FEE150 pKa = 4.03RR151 pKa = 11.84AVITAEE157 pKa = 3.76HH158 pKa = 6.0HH159 pKa = 3.99THH161 pKa = 5.67YY162 pKa = 10.47RR163 pKa = 11.84PEE165 pKa = 4.48EE166 pKa = 3.43IWAIAEE172 pKa = 3.99QHH174 pKa = 4.93TTTLRR179 pKa = 11.84AMGLVLANLRR189 pKa = 11.84KK190 pKa = 9.53IQGITEE196 pKa = 4.02ATVATFLAYY205 pKa = 9.84IMTVRR210 pKa = 11.84PQVAYY215 pKa = 10.38LVATSKK221 pKa = 10.96RR222 pKa = 11.84IWRR225 pKa = 11.84SKK227 pKa = 10.9NITEE231 pKa = 3.89LTEE234 pKa = 3.83ILKK237 pKa = 10.7EE238 pKa = 3.4IATPIKK244 pKa = 10.62SMHH247 pKa = 4.83QHH249 pKa = 6.84EE250 pKa = 4.73ICDD253 pKa = 3.69LTEE256 pKa = 4.52LFEE259 pKa = 4.38LQCLVNRR266 pKa = 11.84GVGQIDD272 pKa = 3.16WRR274 pKa = 11.84KK275 pKa = 8.69EE276 pKa = 3.49RR277 pKa = 11.84SHH279 pKa = 6.05RR280 pKa = 11.84TRR282 pKa = 11.84PDD284 pKa = 3.12VVKK287 pKa = 10.92VSIEE291 pKa = 4.13DD292 pKa = 3.48VVKK295 pKa = 10.3YY296 pKa = 9.28ATEE299 pKa = 3.74IFLLGKK305 pKa = 9.33SHH307 pKa = 6.83GYY309 pKa = 10.02HH310 pKa = 5.13YY311 pKa = 11.21RR312 pKa = 11.84KK313 pKa = 8.94MDD315 pKa = 3.23KK316 pKa = 10.2QKK318 pKa = 10.82YY319 pKa = 9.23ISARR323 pKa = 11.84WEE325 pKa = 3.68WSPTGSIHH333 pKa = 5.86SQYY336 pKa = 10.83PEE338 pKa = 4.33DD339 pKa = 3.95EE340 pKa = 4.35PYY342 pKa = 10.21IPKK345 pKa = 10.43NYY347 pKa = 8.79RR348 pKa = 11.84QKK350 pKa = 10.03TKK352 pKa = 10.42FVALNMMSRR361 pKa = 11.84QHH363 pKa = 6.35VDD365 pKa = 2.47SMFLRR370 pKa = 11.84KK371 pKa = 9.52PEE373 pKa = 3.62IHH375 pKa = 6.53AWTSVKK381 pKa = 10.73YY382 pKa = 9.62EE383 pKa = 3.87WAKK386 pKa = 10.25QRR388 pKa = 11.84AIYY391 pKa = 10.09GVDD394 pKa = 3.3LTSTVITNFAMYY406 pKa = 10.0RR407 pKa = 11.84CEE409 pKa = 4.02EE410 pKa = 4.23VFKK413 pKa = 11.11HH414 pKa = 5.86RR415 pKa = 11.84FPVGEE420 pKa = 3.83EE421 pKa = 3.42AAADD425 pKa = 3.84RR426 pKa = 11.84VHH428 pKa = 6.86RR429 pKa = 11.84RR430 pKa = 11.84LKK432 pKa = 10.67IMLEE436 pKa = 4.24DD437 pKa = 4.03NEE439 pKa = 4.66SFCYY443 pKa = 10.81DD444 pKa = 3.25FDD446 pKa = 5.14DD447 pKa = 5.86FNAQHH452 pKa = 6.09STQAMQAVLVAYY464 pKa = 9.0FNVFQADD471 pKa = 3.29MSDD474 pKa = 3.82DD475 pKa = 3.51QRR477 pKa = 11.84EE478 pKa = 3.98AMLWVIDD485 pKa = 3.96SLSDD489 pKa = 3.6VTIHH493 pKa = 6.46NSNVQPPEE501 pKa = 3.75QYY503 pKa = 9.89EE504 pKa = 4.25LKK506 pKa = 9.94GTLLSGWRR514 pKa = 11.84LTTFMNTALNYY525 pKa = 10.15VYY527 pKa = 10.1FKK529 pKa = 11.06AAGCFDD535 pKa = 3.23IGGVRR540 pKa = 11.84DD541 pKa = 3.85SVDD544 pKa = 3.1NGDD547 pKa = 4.47DD548 pKa = 3.37VLVSIKK554 pKa = 10.59HH555 pKa = 5.48IGAAVRR561 pKa = 11.84IHH563 pKa = 6.5HH564 pKa = 6.82RR565 pKa = 11.84MALINARR572 pKa = 11.84AQPTKK577 pKa = 10.89CNVFSVGEE585 pKa = 3.91FLRR588 pKa = 11.84VEE590 pKa = 4.23HH591 pKa = 7.01KK592 pKa = 10.46VDD594 pKa = 3.32KK595 pKa = 10.22DD596 pKa = 3.38TGLGAQYY603 pKa = 9.02LTRR606 pKa = 11.84ACATLVHH613 pKa = 6.2SRR615 pKa = 11.84CEE617 pKa = 4.19SQEE620 pKa = 4.0PEE622 pKa = 4.31HH623 pKa = 6.18LTGAVKK629 pKa = 10.65AIVTRR634 pKa = 11.84ARR636 pKa = 11.84EE637 pKa = 3.86VFEE640 pKa = 4.02RR641 pKa = 11.84AKK643 pKa = 10.44ISQALLADD651 pKa = 4.33LVRR654 pKa = 11.84SAIRR658 pKa = 11.84RR659 pKa = 11.84AATIFHH665 pKa = 6.86RR666 pKa = 11.84PAKK669 pKa = 9.24EE670 pKa = 3.54AFIIAEE676 pKa = 4.0LHH678 pKa = 5.81AVVGGASTDD687 pKa = 3.51DD688 pKa = 3.63FAPIDD693 pKa = 3.69FKK695 pKa = 11.3INEE698 pKa = 3.88RR699 pKa = 11.84CEE701 pKa = 3.98YY702 pKa = 10.86DD703 pKa = 4.33KK704 pKa = 11.21EE705 pKa = 4.06RR706 pKa = 11.84CANDD710 pKa = 3.56KK711 pKa = 11.1NDD713 pKa = 3.61MVVTQDD719 pKa = 4.21LYY721 pKa = 10.84PGIFDD726 pKa = 3.83YY727 pKa = 11.32ARR729 pKa = 11.84TLAQAYY735 pKa = 9.23EE736 pKa = 4.37GVLDD740 pKa = 3.81EE741 pKa = 4.31QQAKK745 pKa = 9.91RR746 pKa = 11.84RR747 pKa = 11.84VISATTRR754 pKa = 11.84QISVTRR760 pKa = 11.84KK761 pKa = 6.37TWLDD765 pKa = 3.59IVPLAADD772 pKa = 3.79TFYY775 pKa = 11.05RR776 pKa = 11.84YY777 pKa = 9.68GRR779 pKa = 11.84ALYY782 pKa = 10.54KK783 pKa = 9.63MYY785 pKa = 10.33HH786 pKa = 6.28GLVSMPHH793 pKa = 6.11LEE795 pKa = 3.84KK796 pKa = 10.88ARR798 pKa = 11.84FVGIPPIALLDD809 pKa = 3.66SRR811 pKa = 11.84SRR813 pKa = 11.84NMIRR817 pKa = 11.84NIIVEE822 pKa = 4.16ASDD825 pKa = 3.14VEE827 pKa = 4.31YY828 pKa = 10.99ALRR831 pKa = 11.84VLLL834 pKa = 4.86

Molecular weight:
95.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3206

764

1608

1068.7

121.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.858 ± 0.27

1.528 ± 0.106

6.02 ± 0.163

5.864 ± 0.102

4.741 ± 0.352

5.521 ± 0.365

2.682 ± 0.451

5.49 ± 0.478

4.367 ± 0.393

8.079 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.077

3.743 ± 0.201

4.616 ± 0.368

3.462 ± 0.072

7.174 ± 0.13

5.115 ± 0.081

7.112 ± 0.344

7.299 ± 0.34

1.716 ± 0.099

3.93 ± 0.112

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski