Blastococcus sp. DSM 46786

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7T451|A0A1H7T451_9ACTN Cystathionine gamma-lyase OS=Blastococcus sp. DSM 46786 OX=1798227 GN=SAMN04515665_11980 PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84QLLHH6 pKa = 6.66RR7 pKa = 11.84VAPVVVAALVLTGCATGTVRR27 pKa = 11.84GQATPPGDD35 pKa = 3.63GPTDD39 pKa = 3.39VTAGEE44 pKa = 4.41FPVTGAGDD52 pKa = 3.59EE53 pKa = 5.24PIDD56 pKa = 3.64QFARR60 pKa = 11.84NALADD65 pKa = 4.7LDD67 pKa = 4.87AFWAQAYY74 pKa = 9.12PEE76 pKa = 4.39YY77 pKa = 10.4FGEE80 pKa = 4.69EE81 pKa = 4.15YY82 pKa = 10.46TPLAGGYY89 pKa = 9.97FSVDD93 pKa = 2.97SEE95 pKa = 4.6ALDD98 pKa = 3.54EE99 pKa = 4.27SAYY102 pKa = 9.67PGTGIGCEE110 pKa = 4.12GSYY113 pKa = 8.76TAPEE117 pKa = 4.24DD118 pKa = 3.55VAGNAFYY125 pKa = 11.11DD126 pKa = 4.06PVCDD130 pKa = 4.25VIAYY134 pKa = 9.42DD135 pKa = 3.9RR136 pKa = 11.84ALLQEE141 pKa = 4.59LSDD144 pKa = 3.93DD145 pKa = 3.72YY146 pKa = 11.89GRR148 pKa = 11.84FLVPVVMAHH157 pKa = 6.08EE158 pKa = 5.42FGHH161 pKa = 6.59AMQGRR166 pKa = 11.84FGFAEE171 pKa = 4.33SGRR174 pKa = 11.84SIQDD178 pKa = 2.99EE179 pKa = 4.39TQADD183 pKa = 4.26CLAGAWTRR191 pKa = 11.84WVADD195 pKa = 3.23GRR197 pKa = 11.84AAHH200 pKa = 6.64LSLRR204 pKa = 11.84EE205 pKa = 4.06PEE207 pKa = 4.32LDD209 pKa = 3.43DD210 pKa = 3.65VVRR213 pKa = 11.84GFLLLRR219 pKa = 11.84DD220 pKa = 4.48DD221 pKa = 4.65VGSDD225 pKa = 3.48PDD227 pKa = 3.9DD228 pKa = 4.26VEE230 pKa = 4.62AHH232 pKa = 5.93GSYY235 pKa = 10.53FDD237 pKa = 4.04RR238 pKa = 11.84VSAFYY243 pKa = 10.98DD244 pKa = 3.47GFEE247 pKa = 4.57RR248 pKa = 11.84GLGTCRR254 pKa = 11.84DD255 pKa = 3.59AFGEE259 pKa = 4.09DD260 pKa = 3.27RR261 pKa = 11.84LFTAAAFTPTDD272 pKa = 4.19LDD274 pKa = 3.3QGNADD279 pKa = 3.81FADD282 pKa = 3.4IVDD285 pKa = 3.93WVGATLPVFWGEE297 pKa = 3.94VFPAAFGGEE306 pKa = 4.17FEE308 pKa = 4.49EE309 pKa = 4.83PEE311 pKa = 3.65IAGFVGSAPGCDD323 pKa = 3.5GLDD326 pKa = 3.63GRR328 pKa = 11.84DD329 pKa = 3.64LGYY332 pKa = 10.72CADD335 pKa = 4.47DD336 pKa = 3.26RR337 pKa = 11.84TVYY340 pKa = 10.28IDD342 pKa = 3.58EE343 pKa = 4.59TDD345 pKa = 3.84LAVPAYY351 pKa = 10.53DD352 pKa = 4.39EE353 pKa = 4.8IGDD356 pKa = 3.96FALATAMALPYY367 pKa = 10.55ALAVRR372 pKa = 11.84DD373 pKa = 3.9QAGLPVDD380 pKa = 4.78DD381 pKa = 5.58GEE383 pKa = 4.44ATRR386 pKa = 11.84SAVCLTGWYY395 pKa = 8.42QAQWYY400 pKa = 8.51NAEE403 pKa = 3.99FSDD406 pKa = 4.64VVGAEE411 pKa = 4.07ISPGDD416 pKa = 4.05LDD418 pKa = 3.88EE419 pKa = 5.52AVQFLLRR426 pKa = 11.84YY427 pKa = 9.06GVEE430 pKa = 4.21DD431 pKa = 3.69EE432 pKa = 4.81VFPGTDD438 pKa = 2.71ASGFEE443 pKa = 4.14LVGAFRR449 pKa = 11.84NGFLDD454 pKa = 3.98GGAACGIGVV463 pKa = 3.7

Molecular weight: 49.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7SHZ6|A0A1H7SHZ6_9ACTN Uncharacterized protein OS=Blastococcus sp. DSM 46786 OX=1798227 GN=SAMN04515665_11798 PE=4 SV=1
MM1 pKa = 7.44PRR3 pKa = 11.84VTVRR7 pKa = 11.84SVRR10 pKa = 11.84IARR13 pKa = 11.84SARR16 pKa = 11.84TARR19 pKa = 11.84RR20 pKa = 11.84GIVPAVTGRR29 pKa = 11.84TAVPRR34 pKa = 11.84VTVRR38 pKa = 11.84SVRR41 pKa = 11.84TARR44 pKa = 11.84RR45 pKa = 11.84VTGPAVTGRR54 pKa = 11.84TAVRR58 pKa = 3.48

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4401

0

4401

1376897

28

2197

312.9

33.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.452 ± 0.058

0.685 ± 0.012

6.142 ± 0.03

5.728 ± 0.036

2.577 ± 0.025

9.825 ± 0.037

1.985 ± 0.018

2.804 ± 0.026

1.333 ± 0.022

10.758 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.014

1.409 ± 0.017

6.28 ± 0.035

2.682 ± 0.021

8.205 ± 0.041

4.68 ± 0.023

5.863 ± 0.029

9.757 ± 0.038

1.485 ± 0.018

1.74 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski