Spissistilus festinus virus 1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D9D9L8|D9D9L8_9VIRU RNA-directed RNA polymerase (Fragment) OS=Spissistilus festinus virus 1 OX=862943 PE=4 SV=1
MM1 pKa = 7.71SGGDD5 pKa = 3.33LSGPGEE11 pKa = 4.49PLIQPPPVPIPLQEE25 pKa = 5.09DD26 pKa = 4.46GAGSEE31 pKa = 4.68AVPVAPPTRR40 pKa = 11.84ASMEE44 pKa = 4.02ASPGEE49 pKa = 4.1GSPPSSPQGQVQLTGGALGGGDD71 pKa = 4.14PPQAPLWGAQSPKK84 pKa = 10.78GEE86 pKa = 4.26NPTSRR91 pKa = 11.84GRR93 pKa = 11.84SPPPRR98 pKa = 11.84ASPPPEE104 pKa = 4.62SPASQPLAPPPSEE117 pKa = 4.58PNPSIQPPPPSPPPALQPPHH137 pKa = 7.39DD138 pKa = 4.58PQPGPSRR145 pKa = 11.84LPPQHH150 pKa = 6.98PEE152 pKa = 3.51PRR154 pKa = 11.84PPRR157 pKa = 11.84SAGPPPSARR166 pKa = 11.84QQQWYY171 pKa = 8.14TGQTLLYY178 pKa = 9.74NSVPTGRR185 pKa = 11.84EE186 pKa = 4.03SWWGAVARR194 pKa = 11.84VGEE197 pKa = 4.28DD198 pKa = 3.01LGPAVVAIGLGFLQPEE214 pKa = 4.94LLPILPALTAEE225 pKa = 4.52AEE227 pKa = 4.21AEE229 pKa = 3.95IAAATDD235 pKa = 3.62AAAAWAALRR244 pKa = 11.84SHH246 pKa = 7.72LARR249 pKa = 11.84QEE251 pKa = 4.26AEE253 pKa = 3.93ASVPPPEE260 pKa = 4.37NLVDD264 pKa = 3.51ALAWYY269 pKa = 9.7RR270 pKa = 11.84EE271 pKa = 4.19RR272 pKa = 11.84VGRR275 pKa = 11.84PSQHH279 pKa = 6.38PRR281 pKa = 11.84LAIPPHH287 pKa = 6.19RR288 pKa = 11.84LAVEE292 pKa = 3.96AATRR296 pKa = 11.84VPTPTNALGLAITRR310 pKa = 11.84WASHH314 pKa = 6.95LFHH317 pKa = 6.76SHH319 pKa = 6.32PEE321 pKa = 3.85KK322 pKa = 9.63FTKK325 pKa = 10.42VVVGRR330 pKa = 11.84NMFGSSVEE338 pKa = 4.0PHH340 pKa = 6.17HH341 pKa = 7.12TDD343 pKa = 3.17PQDD346 pKa = 3.17TRR348 pKa = 11.84FGRR351 pKa = 11.84RR352 pKa = 11.84DD353 pKa = 3.45KK354 pKa = 10.95QSQPTTVTLGDD365 pKa = 3.7GSEE368 pKa = 4.26SLWMKK373 pKa = 10.58LGEE376 pKa = 4.24HH377 pKa = 6.89SIHH380 pKa = 6.64SANLTANLLVDD391 pKa = 4.07EE392 pKa = 4.79TDD394 pKa = 3.26ISQGVPDD401 pKa = 4.76FCSKK405 pKa = 10.95YY406 pKa = 9.71GTALRR411 pKa = 11.84NNDD414 pKa = 3.15TNFQHH419 pKa = 5.95VANVGLQVGSGVALSTSGGGYY440 pKa = 9.04DD441 pKa = 3.99APVNVWGTVLCTHH454 pKa = 7.14NGPIPLGDD462 pKa = 3.24PVARR466 pKa = 11.84HH467 pKa = 5.93GLHH470 pKa = 5.41VHH472 pKa = 6.68GGPARR477 pKa = 11.84EE478 pKa = 4.16MGRR481 pKa = 11.84FTQTAIASLLQSGVPSEE498 pKa = 4.47RR499 pKa = 11.84FVGSAINPNLAITSLPALKK518 pKa = 10.49NLTASIMEE526 pKa = 3.79GWEE529 pKa = 4.11YY530 pKa = 11.23YY531 pKa = 10.74DD532 pKa = 5.54NSLLYY537 pKa = 10.63AKK539 pKa = 9.9LWHH542 pKa = 6.02YY543 pKa = 11.92VMLNDD548 pKa = 4.57FFSTFGIAPEE558 pKa = 4.14AQAFPQGQGPTWINVTDD575 pKa = 4.34ANLDD579 pKa = 3.44IYY581 pKa = 10.67SIPNAISRR589 pKa = 11.84RR590 pKa = 11.84DD591 pKa = 3.36LVFIEE596 pKa = 4.62GQDD599 pKa = 3.33IFEE602 pKa = 4.44VASDD606 pKa = 3.72LQLIYY611 pKa = 10.24WIVTSAFRR619 pKa = 11.84YY620 pKa = 9.2DD621 pKa = 3.92GPVGAQTPHH630 pKa = 4.85QCYY633 pKa = 9.24TEE635 pKa = 4.19WPQIGVTVLARR646 pKa = 11.84RR647 pKa = 11.84AAPAAPAAAVVPSPTIVSFLTRR669 pKa = 11.84MATRR673 pKa = 11.84RR674 pKa = 11.84NEE676 pKa = 3.54WDD678 pKa = 3.36SALKK682 pKa = 10.54GLYY685 pKa = 10.15LAMEE689 pKa = 4.71LFGVRR694 pKa = 11.84LNMNEE699 pKa = 3.83DD700 pKa = 4.02EE701 pKa = 4.29YY702 pKa = 11.15WPIRR706 pKa = 11.84TCLSAHH712 pKa = 5.99NPWVPKK718 pKa = 9.73VADD721 pKa = 3.58YY722 pKa = 11.81NFMFRR727 pKa = 11.84LLKK730 pKa = 10.21IFPDD734 pKa = 3.18IQGDD738 pKa = 3.8HH739 pKa = 6.49KK740 pKa = 11.02PEE742 pKa = 3.69VGAFISLSASTRR754 pKa = 11.84TRR756 pKa = 11.84VVALYY761 pKa = 9.89NAIMGAAATTVLYY774 pKa = 10.41DD775 pKa = 3.74LNITRR780 pKa = 11.84SLLVSWCTGDD790 pKa = 4.15VDD792 pKa = 4.1TPPTFVQIMTEE803 pKa = 4.19GLNQPQSGNDD813 pKa = 3.53PQEE816 pKa = 4.13CTFFCQARR824 pKa = 11.84AAFPKK829 pKa = 10.11WIGANAASNLFPEE842 pKa = 4.83DD843 pKa = 2.75TWNGDD848 pKa = 3.48FNNNAGAEE856 pKa = 4.22HH857 pKa = 7.13AYY859 pKa = 9.81EE860 pKa = 4.45VYY862 pKa = 10.62NAAITPANFTPLIILEE878 pKa = 4.2WLKK881 pKa = 10.81VLPFEE886 pKa = 5.14WGICSARR893 pKa = 11.84PTLNLRR899 pKa = 11.84EE900 pKa = 4.25EE901 pKa = 3.72IRR903 pKa = 11.84LRR905 pKa = 11.84GLAAAVGWYY914 pKa = 10.39ANRR917 pKa = 11.84GSDD920 pKa = 4.15LYY922 pKa = 10.91RR923 pKa = 11.84EE924 pKa = 4.22RR925 pKa = 11.84AVGDD929 pKa = 3.55FPMKK933 pKa = 9.66VVAYY937 pKa = 9.07GVQVVNALAQGIRR950 pKa = 11.84LAAVPGVGRR959 pKa = 11.84QATIWEE965 pKa = 4.66PYY967 pKa = 9.56GSEE970 pKa = 4.22SGANPPWGPSAAVPAGDD987 pKa = 3.45ISYY990 pKa = 10.71AADD993 pKa = 2.81IHH995 pKa = 5.12TLRR998 pKa = 11.84PCSLMSFDD1006 pKa = 4.3YY1007 pKa = 9.59ATDD1010 pKa = 4.07SVWAPTLLGAALGPGEE1026 pKa = 4.36VQRR1029 pKa = 11.84LTCWTGQTAEE1039 pKa = 4.09MSGVILPMVGIQAPPLRR1056 pKa = 11.84LPGRR1060 pKa = 11.84LNLLRR1065 pKa = 11.84IGGVRR1070 pKa = 11.84ARR1072 pKa = 11.84VPGVDD1077 pKa = 3.75NVPQGAVEE1085 pKa = 4.39VVAPDD1090 pKa = 3.67ALAANPAGNPQQ1101 pKa = 3.03

Molecular weight:
117.76 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D9D9L8|D9D9L8_9VIRU RNA-directed RNA polymerase (Fragment) OS=Spissistilus festinus virus 1 OX=862943 PE=4 SV=1
PP1 pKa = 7.28SISGPSASPLVHH13 pKa = 6.42SGTRR17 pKa = 11.84TTCEE21 pKa = 4.16PQSGLYY27 pKa = 9.46VVYY30 pKa = 10.31DD31 pKa = 3.9VPWLEE36 pKa = 3.97GGGRR40 pKa = 11.84VSLPAGCASSISVARR55 pKa = 11.84EE56 pKa = 3.5VVLLLNYY63 pKa = 10.43LSLGCRR69 pKa = 11.84GRR71 pKa = 11.84AVQDD75 pKa = 3.77SFVSPYY81 pKa = 8.2FTSVVIAPPVGDD93 pKa = 3.75VYY95 pKa = 11.46KK96 pKa = 10.54PGYY99 pKa = 9.9QSATVDD105 pKa = 3.81DD106 pKa = 4.33LTIGVPNFSASEE118 pKa = 4.11LKK120 pKa = 10.42EE121 pKa = 3.75SEE123 pKa = 4.36YY124 pKa = 10.64PGFSAQCLQLLDD136 pKa = 3.98KK137 pKa = 11.03ALSQLMARR145 pKa = 11.84RR146 pKa = 11.84LVTTTQRR153 pKa = 11.84AAIMARR159 pKa = 11.84VFPAYY164 pKa = 9.94QDD166 pKa = 3.24GSRR169 pKa = 11.84PDD171 pKa = 3.24VRR173 pKa = 11.84QQMRR177 pKa = 11.84EE178 pKa = 3.89SVARR182 pKa = 11.84AWALPWTEE190 pKa = 4.9APGDD194 pKa = 4.08PDD196 pKa = 3.23WFTTGGWSYY205 pKa = 11.6ARR207 pKa = 11.84EE208 pKa = 4.28ANSSVVLPTALAALALQSKK227 pKa = 9.18EE228 pKa = 3.93LAKK231 pKa = 10.91VMPNLEE237 pKa = 4.2AQQKK241 pKa = 8.29RR242 pKa = 11.84TFQGSRR248 pKa = 11.84LAGKK252 pKa = 9.55LALLDD257 pKa = 5.31DD258 pKa = 4.54ISALLLAAPRR268 pKa = 11.84DD269 pKa = 3.68TDD271 pKa = 3.72VAQDD275 pKa = 3.77DD276 pKa = 4.23TGADD280 pKa = 3.35APAGLRR286 pKa = 11.84AITSQWLASAVLYY299 pKa = 10.55LAGACTGDD307 pKa = 3.76AAPPLPSLTRR317 pKa = 11.84AWLVQRR323 pKa = 11.84NPALLAQVDD332 pKa = 4.15HH333 pKa = 7.35LDD335 pKa = 4.07GSSSPRR341 pKa = 11.84PYY343 pKa = 10.74VGIGRR348 pKa = 11.84DD349 pKa = 3.32SLIRR353 pKa = 11.84FPGLTLSRR361 pKa = 11.84QTHH364 pKa = 5.57HH365 pKa = 6.58LHH367 pKa = 5.06WAEE370 pKa = 4.04RR371 pKa = 11.84QRR373 pKa = 11.84VCPALVVRR381 pKa = 11.84IRR383 pKa = 11.84SVTARR388 pKa = 11.84RR389 pKa = 11.84HH390 pKa = 4.93LKK392 pKa = 10.0ALNRR396 pKa = 11.84LFPSTSLTDD405 pKa = 3.56SKK407 pKa = 11.3HH408 pKa = 4.02QTTHH412 pKa = 5.75LAEE415 pKa = 4.48VVWSYY420 pKa = 11.22VVGRR424 pKa = 11.84DD425 pKa = 3.78LPGSGHH431 pKa = 5.87PHH433 pKa = 6.96GDD435 pKa = 3.12GQNASKK441 pKa = 10.57SCSPGGGGKK450 pKa = 8.36WFKK453 pKa = 10.86DD454 pKa = 3.03ATASQRR460 pKa = 11.84LRR462 pKa = 11.84AVNAILNHH470 pKa = 5.99IPVGTGQLLTTGAIIAGLGLQHH492 pKa = 7.19CDD494 pKa = 3.57LFRR497 pKa = 11.84DD498 pKa = 4.31TYY500 pKa = 11.18LKK502 pKa = 10.58SGCFWRR508 pKa = 11.84LPHH511 pKa = 6.62QIGGVVKK518 pKa = 10.67AVGTYY523 pKa = 9.09IRR525 pKa = 11.84KK526 pKa = 8.92CAAGLQGNPLSLDD539 pKa = 3.26QVTQLAYY546 pKa = 10.85YY547 pKa = 10.5DD548 pKa = 4.46LLTGRR553 pKa = 11.84SKK555 pKa = 11.25NVTNWQEE562 pKa = 3.91EE563 pKa = 4.02IANRR567 pKa = 11.84CTHH570 pKa = 6.78TYY572 pKa = 9.93HH573 pKa = 7.12IRR575 pKa = 11.84RR576 pKa = 11.84PCLAWQVNDD585 pKa = 4.29KK586 pKa = 11.27GDD588 pKa = 4.13LRR590 pKa = 11.84ATWVEE595 pKa = 4.52DD596 pKa = 3.42SDD598 pKa = 4.15NLNPRR603 pKa = 11.84LMKK606 pKa = 10.18RR607 pKa = 11.84DD608 pKa = 2.85GHH610 pKa = 6.01FYY612 pKa = 10.89SRR614 pKa = 11.84LRR616 pKa = 11.84SILMRR621 pKa = 11.84VCEE624 pKa = 4.21PLVTARR630 pKa = 11.84NTRR633 pKa = 11.84EE634 pKa = 4.05PLDD637 pKa = 3.23KK638 pKa = 10.58FYY640 pKa = 11.36ARR642 pKa = 11.84RR643 pKa = 11.84HH644 pKa = 4.39EE645 pKa = 4.61WIASGSSAGARR656 pKa = 11.84AKK658 pKa = 10.39ISIRR662 pKa = 11.84KK663 pKa = 8.36GRR665 pKa = 11.84LGRR668 pKa = 11.84GSHH671 pKa = 6.54LMAEE675 pKa = 4.39EE676 pKa = 4.24VKK678 pKa = 10.43VSKK681 pKa = 10.49RR682 pKa = 11.84AWAEE686 pKa = 3.69ATPLSSVLEE695 pKa = 4.1ALALSQPRR703 pKa = 11.84EE704 pKa = 3.8VAHH707 pKa = 7.15ASEE710 pKa = 4.85KK711 pKa = 10.53YY712 pKa = 9.81EE713 pKa = 3.99NGKK716 pKa = 9.79ARR718 pKa = 11.84AIYY721 pKa = 9.63GVEE724 pKa = 3.6PMHH727 pKa = 6.49YY728 pKa = 10.28VINTYY733 pKa = 7.81ATKK736 pKa = 10.45GFEE739 pKa = 4.34EE740 pKa = 4.58RR741 pKa = 11.84LHH743 pKa = 6.34LLPGLEE749 pKa = 4.3KK750 pKa = 10.64GASGSQACALEE761 pKa = 4.18VRR763 pKa = 11.84RR764 pKa = 11.84ALITRR769 pKa = 11.84DD770 pKa = 3.42PDD772 pKa = 3.93LEE774 pKa = 4.32CTMLDD779 pKa = 3.37YY780 pKa = 11.66ADD782 pKa = 4.86FNRR785 pKa = 11.84HH786 pKa = 5.2HH787 pKa = 6.46TPEE790 pKa = 4.25AQSLLFDD797 pKa = 3.56VFARR801 pKa = 11.84LGKK804 pKa = 8.88TKK806 pKa = 10.07GAHH809 pKa = 6.46PDD811 pKa = 2.92WVAANLWVARR821 pKa = 11.84AKK823 pKa = 10.21KK824 pKa = 9.59QLWALFPGEE833 pKa = 4.11TEE835 pKa = 3.82PRR837 pKa = 11.84KK838 pKa = 9.94VRR840 pKa = 11.84QGMFSGTRR848 pKa = 11.84STDD851 pKa = 4.15LINTLLNLAYY861 pKa = 10.58FEE863 pKa = 4.42MAKK866 pKa = 10.02EE867 pKa = 3.86WLEE870 pKa = 4.22VQCGLAPTQLYY881 pKa = 9.74HH882 pKa = 5.63VHH884 pKa = 6.3QGDD887 pKa = 4.43DD888 pKa = 3.36VWVSNGNPLWARR900 pKa = 11.84ALYY903 pKa = 10.37YY904 pKa = 10.43CLNNMGFIFQGSKK917 pKa = 9.27QMFGPGRR924 pKa = 11.84GEE926 pKa = 4.0YY927 pKa = 10.66LRR929 pKa = 11.84VLYY932 pKa = 10.8SGGRR936 pKa = 11.84ARR938 pKa = 11.84GYY940 pKa = 9.55FARR943 pKa = 11.84SLANYY948 pKa = 9.77LLRR951 pKa = 11.84PIQNASSADD960 pKa = 3.65PLSWARR966 pKa = 11.84NISDD970 pKa = 3.64GTSLLARR977 pKa = 11.84RR978 pKa = 11.84GLTPRR983 pKa = 11.84MALALYY989 pKa = 9.46MDD991 pKa = 5.17AARR994 pKa = 11.84FWVRR998 pKa = 11.84ARR1000 pKa = 11.84AHH1002 pKa = 6.64PKK1004 pKa = 10.4DD1005 pKa = 3.83NAPVSIPLPVCWLPGIQGGLGSPPPTCLWLPEE1037 pKa = 4.52LDD1039 pKa = 3.8QQLGLALPVMRR1050 pKa = 11.84SSINVEE1056 pKa = 3.89NFTAPTLMTDD1066 pKa = 2.92DD1067 pKa = 4.85WIAHH1071 pKa = 5.15VSKK1074 pKa = 10.42QAYY1077 pKa = 9.16RR1078 pKa = 11.84LTRR1081 pKa = 11.84HH1082 pKa = 6.63PEE1084 pKa = 3.85MPSPNLKK1091 pKa = 9.28MAGLRR1096 pKa = 11.84DD1097 pKa = 4.63DD1098 pKa = 5.54IIMANYY1104 pKa = 9.99VNDD1107 pKa = 3.69LAEE1110 pKa = 4.07YY1111 pKa = 10.09LPEE1114 pKa = 5.11RR1115 pKa = 11.84GWAQYY1120 pKa = 10.21KK1121 pKa = 10.16RR1122 pKa = 11.84DD1123 pKa = 3.45WAEE1126 pKa = 3.52LRR1128 pKa = 11.84TSVGKK1133 pKa = 10.46RR1134 pKa = 11.84LASMSSGAIIASRR1147 pKa = 11.84TEE1149 pKa = 3.67EE1150 pKa = 4.41LGQQLTSIQAWIGYY1164 pKa = 6.99GHH1166 pKa = 7.53PPSHH1170 pKa = 6.63IPAYY1174 pKa = 9.36PYY1176 pKa = 10.66LRR1178 pKa = 11.84SHH1180 pKa = 7.57VDD1182 pKa = 3.28GLDD1185 pKa = 3.36LGEE1188 pKa = 4.64VGFDD1192 pKa = 3.34TPLEE1196 pKa = 3.97NLRR1199 pKa = 11.84DD1200 pKa = 3.88KK1201 pKa = 10.97LSSIITRR1208 pKa = 11.84SVFKK1212 pKa = 11.17NEE1214 pKa = 3.37NTLARR1219 pKa = 11.84VTGLTRR1225 pKa = 11.84LDD1227 pKa = 4.46AISLILAEE1235 pKa = 4.73ADD1237 pKa = 3.28VSGFGDD1243 pKa = 3.54ADD1245 pKa = 3.63VAGVLGPLLTSKK1257 pKa = 10.09NAQMVDD1263 pKa = 3.8LLLGSLGDD1271 pKa = 3.84LVPGLRR1277 pKa = 11.84AYY1279 pKa = 10.64VNNAYY1284 pKa = 7.46WQHH1287 pKa = 5.17VQSVWIAQLTPMAITHH1303 pKa = 6.53PGITPLQCVSADD1315 pKa = 3.6KK1316 pKa = 10.93RR1317 pKa = 11.84GTAQWLYY1324 pKa = 8.01QTIAASQVATSVVYY1338 pKa = 10.58

Molecular weight:
146.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2439

1101

1338

1219.5

132.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.193 ± 0.313

1.23 ± 0.193

4.51 ± 0.307

4.264 ± 0.329

2.542 ± 0.218

8.159 ± 0.281

2.337 ± 0.148

3.854 ± 0.194

2.378 ± 0.608

10.373 ± 1.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.64 ± 0.057

3.649 ± 0.371

8.405 ± 1.764

4.387 ± 0.038

6.437 ± 0.645

7.093 ± 0.331

5.535 ± 0.003

6.683 ± 0.132

2.46 ± 0.005

2.87 ± 0.304

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski