Gata virus
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z2CF63|A0A2Z2CF63_9RHAB Glycoprotein OS=Gata virus OX=1911435 PE=3 SV=1
MM1 pKa = 7.79 KK2 pKa = 9.17 KK3 pKa = 7.62 TQQHH7 pKa = 5.05 SLRR10 pKa = 11.84 PIMDD14 pKa = 3.79 EE15 pKa = 3.9 LNEE18 pKa = 4.09 KK19 pKa = 10.13 FKK21 pKa = 11.35 QFLDD25 pKa = 3.65 DD26 pKa = 3.95 GARR29 pKa = 11.84 QNILSDD35 pKa = 3.37 EE36 pKa = 4.63 FKK38 pKa = 10.85 RR39 pKa = 11.84 DD40 pKa = 3.08 VNAILGEE47 pKa = 4.18 MEE49 pKa = 4.55 DD50 pKa = 4.19 EE51 pKa = 4.39 EE52 pKa = 5.05 MPLAPNDD59 pKa = 3.8 HH60 pKa = 6.38 SPVRR64 pKa = 11.84 QSTSLYY70 pKa = 10.93 SPDD73 pKa = 4.62 HH74 pKa = 7.08 DD75 pKa = 5.13 LDD77 pKa = 3.98 TYY79 pKa = 10.9 TDD81 pKa = 4.42 PGCQSPPGSVCDD93 pKa = 4.13 PLDD96 pKa = 5.06 DD97 pKa = 4.23 EE98 pKa = 5.11 ACSILGEE105 pKa = 4.12 LEE107 pKa = 4.47 RR108 pKa = 11.84 IVTLSDD114 pKa = 4.7 PGNQWEE120 pKa = 4.62 GASHH124 pKa = 6.92 IVWTSPGVYY133 pKa = 8.46 STGRR137 pKa = 11.84 EE138 pKa = 3.82 FQQAKK143 pKa = 8.5 TLLKK147 pKa = 10.57 SVLRR151 pKa = 11.84 SMNRR155 pKa = 11.84 DD156 pKa = 2.86 SGLNYY161 pKa = 9.8 VLNVGKK167 pKa = 9.58 PDD169 pKa = 3.41 TLFITRR175 pKa = 11.84 PDD177 pKa = 3.48 SLTVARR183 pKa = 11.84 NVRR186 pKa = 11.84 PKK188 pKa = 10.16 IPEE191 pKa = 3.83 NLFVDD196 pKa = 3.43 NDD198 pKa = 2.84 KK199 pKa = 11.22 WEE201 pKa = 4.75 FIKK204 pKa = 11.03 AQFASGIEE212 pKa = 4.09 LKK214 pKa = 10.96 DD215 pKa = 3.09 VDD217 pKa = 4.6 EE218 pKa = 4.9 PGKK221 pKa = 8.5 TLVVSFATPGFKK233 pKa = 10.49 EE234 pKa = 3.91 GDD236 pKa = 3.24 IKK238 pKa = 11.29 LSFLNGNTTVRR249 pKa = 11.84 SILEE253 pKa = 4.55 DD254 pKa = 2.4 IWRR257 pKa = 11.84 AMGRR261 pKa = 11.84 WAYY264 pKa = 9.68 ISSSYY269 pKa = 10.55 HH270 pKa = 5.45 VDD272 pKa = 4.22 GIVVV276 pKa = 3.73
Molecular weight: 30.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.797
IPC2_protein 4.749
IPC_protein 4.685
Toseland 4.546
ProMoST 4.813
Dawson 4.66
Bjellqvist 4.8
Wikipedia 4.546
Rodwell 4.546
Grimsley 4.457
Solomon 4.66
Lehninger 4.609
Nozaki 4.762
DTASelect 4.965
Thurlkill 4.546
EMBOSS 4.571
Sillero 4.825
Patrickios 4.164
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.778
Protein with the highest isoelectric point:
>tr|A0A336USW8|A0A336USW8_9RHAB Matrix protein OS=Gata virus OX=1911435 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 9.22 KK3 pKa = 8.4 TRR5 pKa = 11.84 TPNRR9 pKa = 11.84 TEE11 pKa = 3.48 MLSRR15 pKa = 11.84 FRR17 pKa = 11.84 SKK19 pKa = 11.24 NKK21 pKa = 10.6 DD22 pKa = 3.01 NDD24 pKa = 4.13 AGSSDD29 pKa = 3.78 YY30 pKa = 9.9 WANPPNKK37 pKa = 9.05 NPEE40 pKa = 3.74 MSMPNSPVYY49 pKa = 10.92 SFTGSWAISGRR60 pKa = 11.84 FEE62 pKa = 3.92 IRR64 pKa = 11.84 TKK66 pKa = 10.68 IPIQSGAKK74 pKa = 10.21 LLDD77 pKa = 5.24 LIGAWLDD84 pKa = 4.05 GYY86 pKa = 10.67 SGSYY90 pKa = 9.63 SFHH93 pKa = 7.0 GIFTGLYY100 pKa = 9.96 LLMALHH106 pKa = 6.81 SLPSQEE112 pKa = 3.89 NQTYY116 pKa = 10.06 VYY118 pKa = 10.93 SLDD121 pKa = 3.29 LSKK124 pKa = 10.84 RR125 pKa = 11.84 IAIQVTRR132 pKa = 11.84 QDD134 pKa = 4.08 VINQEE139 pKa = 4.58 ADD141 pKa = 3.72 LSWVNQEE148 pKa = 3.8 GTGLEE153 pKa = 3.75 WCVIKK158 pKa = 10.18 FKK160 pKa = 10.65 IHH162 pKa = 6.57 LKK164 pKa = 7.28 PTRR167 pKa = 11.84 RR168 pKa = 11.84 MCIPFADD175 pKa = 4.64 AYY177 pKa = 8.42 KK178 pKa = 10.6 QPMHH182 pKa = 6.84 TGADD186 pKa = 3.52 PPPFEE191 pKa = 3.99 EE192 pKa = 5.24 VYY194 pKa = 10.66 FRR196 pKa = 11.84 FWNPQSGYY204 pKa = 10.67 CSIIHH209 pKa = 6.53
Molecular weight: 23.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.152
IPC2_protein 8.17
IPC_protein 8.185
Toseland 8.448
ProMoST 8.536
Dawson 8.858
Bjellqvist 8.916
Wikipedia 8.946
Rodwell 8.916
Grimsley 8.478
Solomon 9.048
Lehninger 9.019
Nozaki 8.975
DTASelect 8.77
Thurlkill 8.829
EMBOSS 9.019
Sillero 9.048
Patrickios 4.291
IPC_peptide 9.033
IPC2_peptide 7.761
IPC2.peptide.svr19 7.724
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3581
209
2135
716.2
81.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.937 ± 0.682
1.759 ± 0.504
5.892 ± 0.475
4.971 ± 0.23
4.524 ± 0.289
5.781 ± 0.154
2.457 ± 0.317
6.898 ± 0.449
6.171 ± 0.329
9.969 ± 1.135
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.303
5.334 ± 0.4
4.775 ± 0.6
3.239 ± 0.207
5.166 ± 0.456
9.69 ± 0.254
5.613 ± 0.473
5.781 ± 0.476
1.787 ± 0.146
3.658 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here