Water beetle associated circular virus 1
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPC1|A0A346BPC1_9VIRU Putative capsid protein OS=Water beetle associated circular virus 1 OX=2293309 PE=4 SV=1
MM1 pKa = 7.97 EE2 pKa = 5.31 DD3 pKa = 3.44 TLPAKK8 pKa = 10.45 DD9 pKa = 3.78 IGSIPLSPVGLGEE22 pKa = 5.3 RR23 pKa = 11.84 KK24 pKa = 9.7 LKK26 pKa = 11.0 SNNEE30 pKa = 3.98 TFLSDD35 pKa = 3.6 EE36 pKa = 4.3 QEE38 pKa = 4.33 LPTSEE43 pKa = 4.58 SSEE46 pKa = 4.01 HH47 pKa = 6.33 CTDD50 pKa = 3.59 QEE52 pKa = 4.44 PTGSLSTDD60 pKa = 3.18 KK61 pKa = 11.38 LNLAVGQSGILITCDD76 pKa = 2.89 NNPFIEE82 pKa = 4.32 EE83 pKa = 4.18 VTDD86 pKa = 3.73 SEE88 pKa = 4.75 EE89 pKa = 4.79 EE90 pKa = 3.99 IGTDD94 pKa = 3.02 ATDD97 pKa = 3.21 GRR99 pKa = 11.84 RR100 pKa = 11.84 TRR102 pKa = 11.84 KK103 pKa = 9.05 RR104 pKa = 11.84 KK105 pKa = 9.36 RR106 pKa = 11.84 VLRR109 pKa = 11.84 VPKK112 pKa = 10.05 HH113 pKa = 5.56 AIKK116 pKa = 10.14 RR117 pKa = 11.84 WSRR120 pKa = 11.84 GSRR123 pKa = 11.84 QMFKK127 pKa = 9.77 VTLHH131 pKa = 6.73 LPQAYY136 pKa = 9.67 QIATNGVYY144 pKa = 10.31 KK145 pKa = 10.17 GTFGGMAMLQSAEE158 pKa = 4.25 SGEE161 pKa = 4.19 LAQLSAFHH169 pKa = 6.65 HH170 pKa = 5.62 WTPKK174 pKa = 9.55 GWTLRR179 pKa = 11.84 ARR181 pKa = 11.84 KK182 pKa = 8.45 FQLFAQWTTNGTPPTPLGNPVRR204 pKa = 11.84 VRR206 pKa = 11.84 TKK208 pKa = 10.48 VYY210 pKa = 10.5 VYY212 pKa = 9.27 PQAINHH218 pKa = 5.87 IQADD222 pKa = 4.13 PGGEE226 pKa = 3.95 ATPEE230 pKa = 3.9 TLYY233 pKa = 11.39 KK234 pKa = 10.54 NAIGAKK240 pKa = 9.74 ALSTRR245 pKa = 11.84 DD246 pKa = 3.05 RR247 pKa = 11.84 NIRR250 pKa = 11.84 KK251 pKa = 9.25 FIVPAHH257 pKa = 5.59 MRR259 pKa = 11.84 MGAWSAGLFPNFSGQVQDD277 pKa = 3.76 YY278 pKa = 10.35 FKK280 pKa = 10.91 GYY282 pKa = 9.48 YY283 pKa = 10.2 SNFITDD289 pKa = 3.8 GNGFSTDD296 pKa = 3.59 NNAHH300 pKa = 6.9 PWDD303 pKa = 4.01 QNCGVTIEE311 pKa = 4.18 VDD313 pKa = 3.59 VNATIGAVGTGAALALGPQIIHH335 pKa = 7.32 WSTDD339 pKa = 2.42 IYY341 pKa = 11.26 FEE343 pKa = 4.72 AFGNYY348 pKa = 8.79 LFAGII353 pKa = 4.57
Molecular weight: 38.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.409
IPC2_protein 6.313
IPC_protein 6.491
Toseland 6.795
ProMoST 6.868
Dawson 6.868
Bjellqvist 6.766
Wikipedia 6.883
Rodwell 6.854
Grimsley 6.942
Solomon 6.883
Lehninger 6.883
Nozaki 7.088
DTASelect 7.263
Thurlkill 7.293
EMBOSS 7.307
Sillero 7.234
Patrickios 4.418
IPC_peptide 6.898
IPC2_peptide 6.956
IPC2.peptide.svr19 6.91
Protein with the highest isoelectric point:
>tr|A0A346BPC1|A0A346BPC1_9VIRU Putative capsid protein OS=Water beetle associated circular virus 1 OX=2293309 PE=4 SV=1
MM1 pKa = 7.35 AASGRR6 pKa = 11.84 INDD9 pKa = 3.21 IQRR12 pKa = 11.84 RR13 pKa = 11.84 IRR15 pKa = 11.84 ARR17 pKa = 11.84 SWVFTQFVSEE27 pKa = 4.13 EE28 pKa = 4.42 GGWDD32 pKa = 3.62 PNTIDD37 pKa = 3.04 WTATIEE43 pKa = 4.07 PKK45 pKa = 10.42 QVICGLEE52 pKa = 3.81 RR53 pKa = 11.84 GKK55 pKa = 10.5 KK56 pKa = 9.64 GRR58 pKa = 11.84 LHH60 pKa = 5.32 WQGCIFFNNQVTLSTVKK77 pKa = 10.79 AVMDD81 pKa = 4.24 SNNVHH86 pKa = 7.13 LEE88 pKa = 3.74 IKK90 pKa = 10.25 RR91 pKa = 11.84 GNIEE95 pKa = 3.83 QAIKK99 pKa = 10.77 YY100 pKa = 8.39 CLKK103 pKa = 10.76 SEE105 pKa = 4.07 TAEE108 pKa = 3.69 VDD110 pKa = 3.14 EE111 pKa = 5.37 DD112 pKa = 3.93 NEE114 pKa = 4.63 KK115 pKa = 10.35 ILYY118 pKa = 9.5 AYY120 pKa = 9.45 PDD122 pKa = 3.81 IDD124 pKa = 3.25 WQGRR128 pKa = 11.84 LDD130 pKa = 3.88 EE131 pKa = 5.19 DD132 pKa = 3.57 QGKK135 pKa = 9.91 SNSWKK140 pKa = 10.42 LAMEE144 pKa = 4.52 APTYY148 pKa = 10.88 SEE150 pKa = 4.57 GIEE153 pKa = 3.57 ILKK156 pKa = 10.81 ANEE159 pKa = 3.51 PRR161 pKa = 11.84 SWLLYY166 pKa = 9.59 GEE168 pKa = 5.55 RR169 pKa = 11.84 IATNLRR175 pKa = 11.84 HH176 pKa = 5.41 HH177 pKa = 7.09 WEE179 pKa = 3.99 MKK181 pKa = 7.01 TTEE184 pKa = 3.88 EE185 pKa = 4.46 RR186 pKa = 11.84 SPKK189 pKa = 9.26 EE190 pKa = 3.82 YY191 pKa = 10.38 KK192 pKa = 10.37 RR193 pKa = 11.84 PFADD197 pKa = 3.9 KK198 pKa = 10.4 EE199 pKa = 3.99 RR200 pKa = 11.84 LSRR203 pKa = 11.84 KK204 pKa = 9.94 SILLLGQAGTGKK216 pKa = 7.01 TTYY219 pKa = 11.5 AMDD222 pKa = 3.98 HH223 pKa = 6.54 FKK225 pKa = 10.94 HH226 pKa = 6.21 PLLIRR231 pKa = 11.84 HH232 pKa = 5.93 MDD234 pKa = 3.57 GLKK237 pKa = 10.48 KK238 pKa = 9.35 ITPDD242 pKa = 2.99 TDD244 pKa = 3.49 GLVFDD249 pKa = 5.75 DD250 pKa = 6.01 LSFHH254 pKa = 6.46 HH255 pKa = 6.84 WKK257 pKa = 10.11 CAQSVIHH264 pKa = 6.85 LCDD267 pKa = 3.9 LEE269 pKa = 5.04 NDD271 pKa = 3.62 SEE273 pKa = 4.9 INVKK277 pKa = 10.31 HH278 pKa = 5.36 STVTIPKK285 pKa = 9.21 HH286 pKa = 4.39 MPRR289 pKa = 11.84 IFTCNRR295 pKa = 11.84 TFDD298 pKa = 3.19 EE299 pKa = 4.75 WVPEE303 pKa = 4.06 ICKK306 pKa = 10.23 HH307 pKa = 4.75 EE308 pKa = 4.18 EE309 pKa = 3.35 KK310 pKa = 10.24 MAIKK314 pKa = 10.07 RR315 pKa = 11.84 RR316 pKa = 11.84 CDD318 pKa = 3.03 ILIVHH323 pKa = 6.69 NSLFF327 pKa = 3.68
Molecular weight: 38.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.469
IPC2_protein 6.427
IPC_protein 6.547
Toseland 6.707
ProMoST 6.927
Dawson 6.971
Bjellqvist 6.927
Wikipedia 6.971
Rodwell 6.971
Grimsley 6.825
Solomon 6.985
Lehninger 6.985
Nozaki 7.249
DTASelect 7.351
Thurlkill 7.395
EMBOSS 7.38
Sillero 7.38
Patrickios 4.52
IPC_peptide 7.0
IPC2_peptide 7.029
IPC2.peptide.svr19 6.891
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
680
327
353
340.0
38.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.765 ± 1.023
1.618 ± 0.542
5.588 ± 0.545
6.765 ± 0.975
3.971 ± 0.396
7.353 ± 1.408
3.382 ± 0.588
6.765 ± 0.775
6.324 ± 1.064
7.353 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.059 ± 0.253
5.0 ± 0.27
4.706 ± 0.677
3.824 ± 0.5
5.735 ± 0.449
5.882 ± 0.247
7.353 ± 0.808
4.559 ± 0.181
2.5 ± 0.365
2.5 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here