Gordonia phage TimTam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; Nymphadoravirus; unclassified Nymphadoravirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KPB3|A0A516KPB3_9CAUD Helix-turn-helix DNA binding domain protein OS=Gordonia phage TimTam OX=2250398 GN=79 PE=4 SV=1
MM1 pKa = 7.33LASVTLSNPACSYY14 pKa = 7.47TTALPPGYY22 pKa = 10.65VSVDD26 pKa = 3.07PSANVTVTVPLKK38 pKa = 11.14VFTTSLMGWSLPHH51 pKa = 5.8EE52 pKa = 4.47TALGCPSIEE61 pKa = 4.29GPSMADD67 pKa = 3.36CYY69 pKa = 11.4VVDD72 pKa = 5.01FAHH75 pKa = 6.92RR76 pKa = 11.84RR77 pKa = 11.84DD78 pKa = 3.9GGPPLIGPFDD88 pKa = 3.94DD89 pKa = 4.06QIEE92 pKa = 4.01ARR94 pKa = 11.84EE95 pKa = 4.08AGRR98 pKa = 11.84SAKK101 pKa = 10.29AGRR104 pKa = 11.84FDD106 pKa = 3.28IRR108 pKa = 11.84TVYY111 pKa = 10.5PPDD114 pKa = 3.57ATDD117 pKa = 3.13ITPAVDD123 pKa = 3.81PWASSDD129 pKa = 4.47GGDD132 pKa = 3.21TT133 pKa = 3.93

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KP83|A0A516KP83_9CAUD Terminase large subunit OS=Gordonia phage TimTam OX=2250398 GN=2 PE=4 SV=1
MM1 pKa = 7.52GFTSRR6 pKa = 11.84SMYY9 pKa = 11.12AEE11 pKa = 4.07VDD13 pKa = 2.84LDD15 pKa = 3.41MFMRR19 pKa = 11.84ALRR22 pKa = 11.84RR23 pKa = 11.84SGMSYY28 pKa = 10.52QEE30 pKa = 4.47LADD33 pKa = 3.58EE34 pKa = 4.56ATRR37 pKa = 11.84EE38 pKa = 3.91LRR40 pKa = 11.84KK41 pKa = 9.31IARR44 pKa = 11.84SEE46 pKa = 3.81RR47 pKa = 11.84RR48 pKa = 11.84KK49 pKa = 9.96RR50 pKa = 11.84RR51 pKa = 11.84PDD53 pKa = 3.27NVPAGVSKK61 pKa = 10.98ALIGQIVNGAAKK73 pKa = 8.95STHH76 pKa = 5.73EE77 pKa = 4.0LRR79 pKa = 11.84GLAIEE84 pKa = 4.5RR85 pKa = 11.84ALEE88 pKa = 4.03VSDD91 pKa = 4.52GDD93 pKa = 3.95LFVPRR98 pKa = 11.84VVRR101 pKa = 11.84GSGTTNRR108 pKa = 11.84RR109 pKa = 11.84VASS112 pKa = 3.73

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

17187

32

2142

209.6

22.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.928 ± 0.616

0.826 ± 0.159

7.005 ± 0.359

5.696 ± 0.294

2.653 ± 0.178

8.483 ± 0.337

1.978 ± 0.168

4.486 ± 0.179

3.183 ± 0.196

7.57 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.177

2.95 ± 0.185

5.673 ± 0.268

3.66 ± 0.273

7.866 ± 0.438

5.382 ± 0.221

7.18 ± 0.343

7.337 ± 0.228

1.955 ± 0.13

2.106 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski