Gordonia phage Lauer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VJ61|A0A649VJ61_9CAUD Uncharacterized protein OS=Gordonia phage Lauer OX=2656538 GN=30 PE=4 SV=1
MM1 pKa = 7.3NKK3 pKa = 10.2KK4 pKa = 10.46LITILAGVVAACGMTAAPATAMISLDD30 pKa = 3.09AKK32 pKa = 10.61SNVRR36 pKa = 11.84YY37 pKa = 7.78MVCGDD42 pKa = 3.57GVAEE46 pKa = 3.89IEE48 pKa = 4.38YY49 pKa = 10.67NNAYY53 pKa = 10.25GNYY56 pKa = 9.38DD57 pKa = 3.24WADD60 pKa = 2.98VDD62 pKa = 5.38LSQGCWFYY70 pKa = 11.74DD71 pKa = 3.89LYY73 pKa = 11.42NGSDD77 pKa = 3.27EE78 pKa = 4.88FGYY81 pKa = 10.15PDD83 pKa = 3.64GAAWADD89 pKa = 3.64VNVTDD94 pKa = 4.08TNGGYY99 pKa = 9.57VSCVVWVNGLIEE111 pKa = 4.8GRR113 pKa = 11.84ASDD116 pKa = 3.35NSEE119 pKa = 4.01YY120 pKa = 10.91YY121 pKa = 10.62SYY123 pKa = 11.49ASCYY127 pKa = 9.91

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VIN3|A0A649VIN3_9CAUD Uncharacterized protein OS=Gordonia phage Lauer OX=2656538 GN=61 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84PVSVGFLGVGVLEE15 pKa = 4.73SKK17 pKa = 10.71RR18 pKa = 11.84STRR21 pKa = 11.84TPRR24 pKa = 11.84KK25 pKa = 8.23DD26 pKa = 2.88HH27 pKa = 5.74TMAMSHH33 pKa = 6.7ANCSHH38 pKa = 7.24PKK40 pKa = 9.04TPAARR45 pKa = 11.84AACRR49 pKa = 11.84KK50 pKa = 9.72GRR52 pKa = 11.84TQQPTVPAIDD62 pKa = 3.83LLNRR66 pKa = 11.84TLEE69 pKa = 4.38GEE71 pKa = 4.13LLNVQRR77 pKa = 11.84RR78 pKa = 11.84AATAALRR85 pKa = 11.84DD86 pKa = 3.8SAQIDD91 pKa = 3.41PAAITRR97 pKa = 11.84VRR99 pKa = 11.84EE100 pKa = 3.54IAYY103 pKa = 7.12EE104 pKa = 4.05TRR106 pKa = 11.84EE107 pKa = 3.65QAARR111 pKa = 11.84QGFKK115 pKa = 10.88HH116 pKa = 6.16MDD118 pKa = 3.21RR119 pKa = 11.84SDD121 pKa = 3.24VRR123 pKa = 11.84DD124 pKa = 3.54VFDD127 pKa = 4.86AMTSVLEE134 pKa = 3.88LHH136 pKa = 6.39GWRR139 pKa = 11.84LKK141 pKa = 10.52FDD143 pKa = 3.63NARR146 pKa = 11.84RR147 pKa = 11.84RR148 pKa = 11.84AGQCNYY154 pKa = 10.21SMRR157 pKa = 11.84TISISAPLLEE167 pKa = 4.41VRR169 pKa = 11.84TFADD173 pKa = 3.27TLEE176 pKa = 4.78TITHH180 pKa = 6.39EE181 pKa = 4.23LAHH184 pKa = 6.48AVTPGHH190 pKa = 5.61NHH192 pKa = 7.09DD193 pKa = 4.12YY194 pKa = 9.45TWKK197 pKa = 10.45KK198 pKa = 6.62MHH200 pKa = 7.3RR201 pKa = 11.84DD202 pKa = 3.11MGGNGSTRR210 pKa = 11.84YY211 pKa = 10.65NMDD214 pKa = 3.05EE215 pKa = 4.14DD216 pKa = 3.75TRR218 pKa = 11.84ARR220 pKa = 11.84ALATARR226 pKa = 11.84YY227 pKa = 9.38IGTCAHH233 pKa = 7.0GKK235 pKa = 8.7QFPRR239 pKa = 11.84QSAPKK244 pKa = 8.89RR245 pKa = 11.84GSSHH249 pKa = 5.55VCKK252 pKa = 10.26CPEE255 pKa = 3.62GRR257 pKa = 11.84TTVTWARR264 pKa = 11.84NFF266 pKa = 3.41

Molecular weight: 29.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

15502

53

1694

234.9

26.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.696 ± 0.592

1.09 ± 0.188

6.735 ± 0.321

5.857 ± 0.395

3.2 ± 0.186

8.115 ± 0.612

2.071 ± 0.185

4.935 ± 0.184

4.741 ± 0.284

7.58 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.146

3.529 ± 0.237

5.419 ± 0.284

3.787 ± 0.266

6.728 ± 0.339

5.851 ± 0.312

5.896 ± 0.318

7.102 ± 0.25

2.167 ± 0.133

2.819 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski