Olavius algarvensis Delta 1 endosymbiont
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12909 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J4XYF8|A0A6J4XYF8_9DELT Uncharacterized protein OS=Olavius algarvensis Delta 1 endosymbiont OX=334747 GN=D1AOALGA4SA_4813 PE=4 SV=1
MM1 pKa = 7.42 GRR3 pKa = 11.84 AKK5 pKa = 10.59 QNLLQDD11 pKa = 3.99 NPQQTEE17 pKa = 3.86 VSRR20 pKa = 11.84 TFLLFGDD27 pKa = 4.11 PAMVLQVPLPRR38 pKa = 11.84 RR39 pKa = 11.84 PEE41 pKa = 3.95 GLTAVLNADD50 pKa = 3.64 TTVEE54 pKa = 4.32 LNWDD58 pKa = 3.63 SALDD62 pKa = 3.99 ANGGAAAGYY71 pKa = 9.86 NIYY74 pKa = 10.42 RR75 pKa = 11.84 GPWPNGPWTQLNDD88 pKa = 3.5 TALMDD93 pKa = 3.94 TVYY96 pKa = 10.98 SDD98 pKa = 4.32 SDD100 pKa = 3.83 LTSGAAYY107 pKa = 9.91 YY108 pKa = 10.28 YY109 pKa = 11.07 VVTALDD115 pKa = 3.69 ADD117 pKa = 4.14 GDD119 pKa = 3.99 EE120 pKa = 4.97 SVYY123 pKa = 10.97 SDD125 pKa = 3.78 PASAVLTDD133 pKa = 3.43 SDD135 pKa = 4.76 GDD137 pKa = 3.79 GLYY140 pKa = 10.95 DD141 pKa = 3.76 LLEE144 pKa = 4.42 AAGCTDD150 pKa = 3.63 GNMSDD155 pKa = 3.74 SDD157 pKa = 4.27 GDD159 pKa = 3.98 GLSDD163 pKa = 3.47 GTEE166 pKa = 3.79 DD167 pKa = 3.74 ANRR170 pKa = 11.84 NGTLDD175 pKa = 3.49 AGEE178 pKa = 4.82 TDD180 pKa = 3.64 PCDD183 pKa = 3.86 ADD185 pKa = 3.91 SDD187 pKa = 4.83 ADD189 pKa = 3.94 TLPDD193 pKa = 3.07 GWEE196 pKa = 3.86 TANGFNALSGSGDD209 pKa = 3.71 DD210 pKa = 5.75 DD211 pKa = 4.37 AAADD215 pKa = 4.06 PDD217 pKa = 4.17 GDD219 pKa = 3.1 GWTNYY224 pKa = 8.77 QEE226 pKa = 4.58 YY227 pKa = 10.56 LSCTDD232 pKa = 3.67 PADD235 pKa = 3.95 AASVPAAPTVPALNYY250 pKa = 7.33 PANGSEE256 pKa = 4.17 TTTLDD261 pKa = 2.85 PWLSVSNAADD271 pKa = 4.15 ADD273 pKa = 4.25 CQDD276 pKa = 3.06 LRR278 pKa = 11.84 YY279 pKa = 10.34 VFEE282 pKa = 5.15 LYY284 pKa = 10.93 ADD286 pKa = 4.1 AGLTTLVDD294 pKa = 3.41 ATAAEE299 pKa = 5.42 GIAEE303 pKa = 4.46 GDD305 pKa = 3.62 NTTAWQPGSALNEE318 pKa = 3.6 NAQYY322 pKa = 10.55 YY323 pKa = 7.91 WRR325 pKa = 11.84 ARR327 pKa = 11.84 AFDD330 pKa = 4.11 SLASSDD336 pKa = 3.71 WLATAAFFVNTTAEE350 pKa = 4.31 APSTPAVSAPPDD362 pKa = 3.6 GSEE365 pKa = 3.91 VSVRR369 pKa = 11.84 RR370 pKa = 11.84 PSLEE374 pKa = 3.88 VINAVDD380 pKa = 4.03 ADD382 pKa = 4.28 GDD384 pKa = 3.95 PLTYY388 pKa = 10.75 AFRR391 pKa = 11.84 VYY393 pKa = 11.0 ADD395 pKa = 4.05 EE396 pKa = 4.23 NLEE399 pKa = 4.19 TLVDD403 pKa = 3.93 SQSAVAEE410 pKa = 4.43 GSGATTAWQVAAEE423 pKa = 4.45 LDD425 pKa = 4.18 DD426 pKa = 4.01 NTDD429 pKa = 3.35 YY430 pKa = 10.74 WWTVQATDD438 pKa = 5.37 DD439 pKa = 4.17 EE440 pKa = 5.17 DD441 pKa = 4.07 LTGDD445 pKa = 3.15 WSVADD450 pKa = 3.7 RR451 pKa = 11.84 FFVNTANDD459 pKa = 3.68 APTTPPGGSPAIGAEE474 pKa = 4.15 LATTTPLLAVVHH486 pKa = 5.93 ATDD489 pKa = 4.39 ADD491 pKa = 4.29 LDD493 pKa = 3.85 ALSYY497 pKa = 10.62 FFEE500 pKa = 6.19 IDD502 pKa = 2.87 TAATFDD508 pKa = 4.05 SFEE511 pKa = 4.74 LEE513 pKa = 4.1 QSDD516 pKa = 4.53 EE517 pKa = 4.24 LTEE520 pKa = 3.99 EE521 pKa = 4.59 AGVSTSWATQEE532 pKa = 5.65 LIDD535 pKa = 3.59 NTTYY539 pKa = 9.69 YY540 pKa = 9.66 WRR542 pKa = 11.84 VRR544 pKa = 11.84 AFDD547 pKa = 3.61 GAAYY551 pKa = 9.64 GPWHH555 pKa = 6.06 NASFFVNTANDD566 pKa = 3.54 APGAPTVDD574 pKa = 3.46 HH575 pKa = 7.18 PGDD578 pKa = 3.69 GSEE581 pKa = 4.41 VSTLQPTLTVKK592 pKa = 10.7 AAIDD596 pKa = 3.66 VDD598 pKa = 4.84 LDD600 pKa = 3.52 ALVYY604 pKa = 10.69 DD605 pKa = 4.27 FEE607 pKa = 6.12 LYY609 pKa = 10.7 EE610 pKa = 4.33 DD611 pKa = 4.29 TEE613 pKa = 4.41 LTTLVTDD620 pKa = 3.74 VAGVGLAWQVDD631 pKa = 3.76 VTLDD635 pKa = 3.62 DD636 pKa = 3.48 NRR638 pKa = 11.84 SYY640 pKa = 10.94 FWRR643 pKa = 11.84 ARR645 pKa = 11.84 AVDD648 pKa = 3.37 AHH650 pKa = 6.4 GAAGPWSVLVSFFVNTANDD669 pKa = 4.0 TPGTPVLNNPLSGGIATALTPTLSVFNADD698 pKa = 5.03 DD699 pKa = 3.8 TDD701 pKa = 3.92 QDD703 pKa = 3.59 ALTYY707 pKa = 10.81 AFEE710 pKa = 4.95 LYY712 pKa = 10.94 ADD714 pKa = 4.49 ADD716 pKa = 3.98 LSQLVVGAFVAEE728 pKa = 4.19 GHH730 pKa = 7.32 LITSWTVSATLDD742 pKa = 4.14 DD743 pKa = 3.74 NGIYY747 pKa = 9.91 YY748 pKa = 9.52 WRR750 pKa = 11.84 ARR752 pKa = 11.84 ADD754 pKa = 3.93 DD755 pKa = 4.37 GQHH758 pKa = 6.39 PGAWMPTAVLEE769 pKa = 4.02 IHH771 pKa = 6.17 TAGTNTAYY779 pKa = 10.12 EE780 pKa = 4.35 IVTQQPVSAAATARR794 pKa = 11.84 QTVSVSAVDD803 pKa = 3.4 HH804 pKa = 6.71 PIYY807 pKa = 10.07 NTVVEE812 pKa = 5.03 LPPGALPRR820 pKa = 11.84 DD821 pKa = 3.78 CSINIGPVTNPPALPRR837 pKa = 11.84 GARR840 pKa = 11.84 KK841 pKa = 9.22 IGHH844 pKa = 4.79 VTEE847 pKa = 5.5 FGPSGMTFNVPVVIKK862 pKa = 10.63 LPYY865 pKa = 8.8 TADD868 pKa = 3.43 ALNQARR874 pKa = 11.84 VANPVEE880 pKa = 4.33 LSVYY884 pKa = 9.64 WYY886 pKa = 10.24 DD887 pKa = 3.48 PSLLAWVPVEE897 pKa = 4.26 ILDD900 pKa = 3.65 IDD902 pKa = 4.8 RR903 pKa = 11.84 INKK906 pKa = 8.9 LVSIEE911 pKa = 3.96 TDD913 pKa = 2.98 HH914 pKa = 7.65 FSMYY918 pKa = 10.38 TIGADD923 pKa = 3.26 VADD926 pKa = 4.15 ASGGGGGTCFIKK938 pKa = 10.45 AVHH941 pKa = 6.12 RR942 pKa = 11.84 AEE944 pKa = 4.04 TVFFIGNWSDD954 pKa = 3.17 ISICGLLALVGLLWMGRR971 pKa = 11.84 RR972 pKa = 11.84 RR973 pKa = 11.84 RR974 pKa = 11.84 HH975 pKa = 4.81
Molecular weight: 103.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 1.087
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.727
Protein with the highest isoelectric point:
>tr|A0A6J4YHL3|A0A6J4YHL3_9DELT Oxidoreductase short-chain dehydrogenase/reductase family OS=Olavius algarvensis Delta 1 endosymbiont OX=334747 GN=D1AOALGA4SA_9704 PE=4 SV=1
QQ1 pKa = 7.1 PATRR5 pKa = 11.84 NPQRR9 pKa = 11.84 ATRR12 pKa = 11.84 NPQRR16 pKa = 11.84 ATRR19 pKa = 11.84 NPQPATRR26 pKa = 11.84 NAQPATRR33 pKa = 11.84 NAQRR37 pKa = 11.84 ATRR40 pKa = 11.84 NPNPQLATRR49 pKa = 11.84 NPQPVTRR56 pKa = 11.84 NPKK59 pKa = 8.86 PATRR63 pKa = 11.84 NPQSAIRR70 pKa = 11.84 NPHH73 pKa = 5.43 SAIHH77 pKa = 5.67 SS78 pKa = 3.82
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.618
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.34
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.251
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12909
0
12909
2800634
20
4859
217.0
24.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.561 ± 0.029
1.255 ± 0.012
5.574 ± 0.026
5.955 ± 0.022
4.46 ± 0.018
7.331 ± 0.023
2.069 ± 0.011
6.964 ± 0.023
5.434 ± 0.029
9.868 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.619 ± 0.014
3.846 ± 0.02
4.515 ± 0.018
3.694 ± 0.016
5.869 ± 0.024
6.029 ± 0.02
4.96 ± 0.024
6.659 ± 0.019
1.238 ± 0.01
3.101 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here