Amino acid dipepetide frequency for Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.842AlaAla: 16.842 ± 0.163	1.088AlaCys: 1.088 ± 0.034	6.824AlaAsp: 6.824 ± 0.086	6.545AlaGlu: 6.545 ± 0.081	4.228AlaPhe: 4.228 ± 0.063	9.898AlaGly: 9.898 ± 0.107	2.179AlaHis: 2.179 ± 0.043	6.652AlaIle: 6.652 ± 0.078	4.625AlaLys: 4.625 ± 0.081	12.261AlaLeu: 12.261 ± 0.116	3.254AlaMet: 3.254 ± 0.053	3.167AlaAsn: 3.167 ± 0.062	5.607AlaPro: 5.607 ± 0.085	3.93AlaGln: 3.93 ± 0.059	8.912AlaArg: 8.912 ± 0.102	6.941AlaSer: 6.941 ± 0.093	6.429AlaThr: 6.429 ± 0.081	8.56AlaVal: 8.56 ± 0.089	1.428AlaTrp: 1.428 ± 0.034	2.416AlaTyr: 2.416 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.992CysAla: 0.992 ± 0.034	0.139CysCys: 0.139 ± 0.01	0.554CysAsp: 0.554 ± 0.02	0.478CysGlu: 0.478 ± 0.021	0.325CysPhe: 0.325 ± 0.017	0.877CysGly: 0.877 ± 0.029	0.25CysHis: 0.25 ± 0.016	0.431CysIle: 0.431 ± 0.019	0.248CysLys: 0.248 ± 0.014	0.752CysLeu: 0.752 ± 0.025	0.153CysMet: 0.153 ± 0.011	0.219CysAsn: 0.219 ± 0.016	0.469CysPro: 0.469 ± 0.023	0.242CysGln: 0.242 ± 0.015	0.69CysArg: 0.69 ± 0.024	0.509CysSer: 0.509 ± 0.021	0.445CysThr: 0.445 ± 0.019	0.599CysVal: 0.599 ± 0.023	0.129CysTrp: 0.129 ± 0.012	0.218CysTyr: 0.218 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.967AspAla: 6.967 ± 0.086	0.492AspCys: 0.492 ± 0.021	3.56AspAsp: 3.56 ± 0.059	3.353AspGlu: 3.353 ± 0.055	2.11AspPhe: 2.11 ± 0.044	5.088AspGly: 5.088 ± 0.077	1.405AspHis: 1.405 ± 0.034	3.234AspIle: 3.234 ± 0.053	2.064AspLys: 2.064 ± 0.04	5.743AspLeu: 5.743 ± 0.066	1.221AspMet: 1.221 ± 0.028	1.504AspAsn: 1.504 ± 0.037	3.388AspPro: 3.388 ± 0.052	1.859AspGln: 1.859 ± 0.043	4.555AspArg: 4.555 ± 0.067	2.485AspSer: 2.485 ± 0.048	2.677AspThr: 2.677 ± 0.048	4.19AspVal: 4.19 ± 0.059	0.868AspTrp: 0.868 ± 0.031	1.452AspTyr: 1.452 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.661GluAla: 6.661 ± 0.08	0.36GluCys: 0.36 ± 0.016	2.405GluAsp: 2.405 ± 0.046	2.543GluGlu: 2.543 ± 0.049	1.807GluPhe: 1.807 ± 0.042	3.603GluGly: 3.603 ± 0.06	1.18GluHis: 1.18 ± 0.027	3.417GluIle: 3.417 ± 0.054	2.314GluLys: 2.314 ± 0.047	4.95GluLeu: 4.95 ± 0.064	1.378GluMet: 1.378 ± 0.037	1.533GluAsn: 1.533 ± 0.035	2.541GluPro: 2.541 ± 0.046	2.015GluGln: 2.015 ± 0.043	4.948GluArg: 4.948 ± 0.071	2.498GluSer: 2.498 ± 0.046	3.274GluThr: 3.274 ± 0.055	3.517GluVal: 3.517 ± 0.055	0.709GluTrp: 0.709 ± 0.025	0.958GluTyr: 0.958 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.47PheAla: 4.47 ± 0.059	0.396PheCys: 0.396 ± 0.02	2.567PheAsp: 2.567 ± 0.041	2.079PheGlu: 2.079 ± 0.041	1.364PhePhe: 1.364 ± 0.039	3.513PheGly: 3.513 ± 0.063	0.784PheHis: 0.784 ± 0.024	1.62PheIle: 1.62 ± 0.038	1.185PheLys: 1.185 ± 0.035	3.152PheLeu: 3.152 ± 0.064	0.741PheMet: 0.741 ± 0.025	1.17PheAsn: 1.17 ± 0.036	1.579PhePro: 1.579 ± 0.039	1.001PheGln: 1.001 ± 0.033	2.434PheArg: 2.434 ± 0.048	2.21PheSer: 2.21 ± 0.047	1.941PheThr: 1.941 ± 0.04	2.876PheVal: 2.876 ± 0.051	0.487PheTrp: 0.487 ± 0.023	0.845PheTyr: 0.845 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	8.611GlyAla: 8.611 ± 0.102	0.802GlyCys: 0.802 ± 0.026	4.361GlyAsp: 4.361 ± 0.065	4.238GlyGlu: 4.238 ± 0.067	3.515GlyPhe: 3.515 ± 0.061	7.132GlyGly: 7.132 ± 0.136	1.943GlyHis: 1.943 ± 0.044	4.519GlyIle: 4.519 ± 0.062	3.407GlyLys: 3.407 ± 0.056	7.956GlyLeu: 7.956 ± 0.109	2.021GlyMet: 2.021 ± 0.04	2.365GlyAsn: 2.365 ± 0.051	3.318GlyPro: 3.318 ± 0.058	2.668GlyGln: 2.668 ± 0.042	6.125GlyArg: 6.125 ± 0.066	4.755GlySer: 4.755 ± 0.072	4.6GlyThr: 4.6 ± 0.085	5.826GlyVal: 5.826 ± 0.074	1.284GlyTrp: 1.284 ± 0.037	2.238GlyTyr: 2.238 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.343HisAla: 2.343 ± 0.043	0.25HisCys: 0.25 ± 0.014	1.336HisAsp: 1.336 ± 0.035	1.061HisGlu: 1.061 ± 0.034	0.793HisPhe: 0.793 ± 0.025	2.066HisGly: 2.066 ± 0.044	0.662HisHis: 0.662 ± 0.03	0.978HisIle: 0.978 ± 0.028	0.582HisLys: 0.582 ± 0.023	2.067HisLeu: 2.067 ± 0.046	0.453HisMet: 0.453 ± 0.018	0.55HisAsn: 0.55 ± 0.022	1.368HisPro: 1.368 ± 0.036	0.615HisGln: 0.615 ± 0.024	1.709HisArg: 1.709 ± 0.04	1.072HisSer: 1.072 ± 0.028	0.879HisThr: 0.879 ± 0.029	1.507HisVal: 1.507 ± 0.032	0.348HisTrp: 0.348 ± 0.016	0.522HisTyr: 0.522 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.396IleAla: 7.396 ± 0.084	0.508IleCys: 0.508 ± 0.019	4.017IleAsp: 4.017 ± 0.056	3.647IleGlu: 3.647 ± 0.055	1.697IlePhe: 1.697 ± 0.035	4.892IleGly: 4.892 ± 0.07	0.982IleHis: 0.982 ± 0.027	2.307IleIle: 2.307 ± 0.049	1.859IleLys: 1.859 ± 0.038	4.128IleLeu: 4.128 ± 0.065	0.941IleMet: 0.941 ± 0.028	1.532IleAsn: 1.532 ± 0.037	2.406IlePro: 2.406 ± 0.04	1.27IleGln: 1.27 ± 0.03	3.418IleArg: 3.418 ± 0.058	3.048IleSer: 3.048 ± 0.057	2.676IleThr: 2.676 ± 0.045	4.668IleVal: 4.668 ± 0.066	0.61IleTrp: 0.61 ± 0.025	1.169IleTyr: 1.169 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.626LysAla: 4.626 ± 0.077	0.221LysCys: 0.221 ± 0.014	1.97LysAsp: 1.97 ± 0.044	1.696LysGlu: 1.696 ± 0.04	1.074LysPhe: 1.074 ± 0.028	2.744LysGly: 2.744 ± 0.051	0.77LysHis: 0.77 ± 0.026	1.918LysIle: 1.918 ± 0.043	1.722LysLys: 1.722 ± 0.06	3.745LysLeu: 3.745 ± 0.066	0.878LysMet: 0.878 ± 0.027	1.072LysAsn: 1.072 ± 0.032	2.465LysPro: 2.465 ± 0.051	1.309LysGln: 1.309 ± 0.03	2.811LysArg: 2.811 ± 0.049	2.297LysSer: 2.297 ± 0.048	2.398LysThr: 2.398 ± 0.045	2.605LysVal: 2.605 ± 0.045	0.442LysTrp: 0.442 ± 0.019	0.739LysTyr: 0.739 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	12.535LeuAla: 12.535 ± 0.127	0.851LeuCys: 0.851 ± 0.023	5.735LeuAsp: 5.735 ± 0.074	4.696LeuGlu: 4.696 ± 0.067	3.381LeuPhe: 3.381 ± 0.053	7.248LeuGly: 7.248 ± 0.084	1.899LeuHis: 1.899 ± 0.04	4.958LeuIle: 4.958 ± 0.075	3.932LeuLys: 3.932 ± 0.064	8.893LeuLeu: 8.893 ± 0.117	2.237LeuMet: 2.237 ± 0.043	2.688LeuAsn: 2.688 ± 0.044	5.289LeuPro: 5.289 ± 0.065	2.721LeuGln: 2.721 ± 0.045	7.042LeuArg: 7.042 ± 0.072	6.17LeuSer: 6.17 ± 0.076	5.614LeuThr: 5.614 ± 0.075	7.125LeuVal: 7.125 ± 0.094	1.091LeuTrp: 1.091 ± 0.038	1.871LeuTyr: 1.871 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	2.736MetAla: 2.736 ± 0.051	0.157MetCys: 0.157 ± 0.012	1.018MetAsp: 1.018 ± 0.029	0.958MetGlu: 0.958 ± 0.028	0.693MetPhe: 0.693 ± 0.025	1.428MetGly: 1.428 ± 0.034	0.421MetHis: 0.421 ± 0.018	1.333MetIle: 1.333 ± 0.033	1.035MetLys: 1.035 ± 0.026	2.295MetLeu: 2.295 ± 0.038	0.68MetMet: 0.68 ± 0.025	0.694MetAsn: 0.694 ± 0.021	1.532MetPro: 1.532 ± 0.037	0.814MetGln: 0.814 ± 0.023	1.861MetArg: 1.861 ± 0.04	1.68MetSer: 1.68 ± 0.037	1.898MetThr: 1.898 ± 0.036	1.623MetVal: 1.623 ± 0.035	0.224MetTrp: 0.224 ± 0.013	0.314MetTyr: 0.314 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.391AsnAla: 3.391 ± 0.056	0.245AsnCys: 0.245 ± 0.014	1.63AsnAsp: 1.63 ± 0.037	1.462AsnGlu: 1.462 ± 0.03	1.052AsnPhe: 1.052 ± 0.031	2.563AsnGly: 2.563 ± 0.06	0.542AsnHis: 0.542 ± 0.023	1.452AsnIle: 1.452 ± 0.033	0.871AsnLys: 0.871 ± 0.025	2.647AsnLeu: 2.647 ± 0.048	0.578AsnMet: 0.578 ± 0.02	0.858AsnAsn: 0.858 ± 0.037	1.89AsnPro: 1.89 ± 0.04	0.849AsnGln: 0.849 ± 0.027	1.955AsnArg: 1.955 ± 0.04	1.48AsnSer: 1.48 ± 0.041	1.375AsnThr: 1.375 ± 0.038	2.171AsnVal: 2.171 ± 0.043	0.462AsnTrp: 0.462 ± 0.018	0.718AsnTyr: 0.718 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	6.468ProAla: 6.468 ± 0.086	0.354ProCys: 0.354 ± 0.018	3.753ProAsp: 3.753 ± 0.057	3.12ProGlu: 3.12 ± 0.047	1.825ProPhe: 1.825 ± 0.038	4.258ProGly: 4.258 ± 0.06	1.107ProHis: 1.107 ± 0.03	2.341ProIle: 2.341 ± 0.042	2.038ProLys: 2.038 ± 0.046	4.546ProLeu: 4.546 ± 0.074	1.17ProMet: 1.17 ± 0.032	1.445ProAsn: 1.445 ± 0.038	3.116ProPro: 3.116 ± 0.081	1.796ProGln: 1.796 ± 0.043	3.385ProArg: 3.385 ± 0.067	3.188ProSer: 3.188 ± 0.053	2.569ProThr: 2.569 ± 0.051	4.076ProVal: 4.076 ± 0.061	0.68ProTrp: 0.68 ± 0.022	1.173ProTyr: 1.173 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.84GlnAla: 3.84 ± 0.059	0.237GlnCys: 0.237 ± 0.014	1.421GlnAsp: 1.421 ± 0.038	1.274GlnGlu: 1.274 ± 0.036	1.148GlnPhe: 1.148 ± 0.025	2.236GlnGly: 2.236 ± 0.047	0.743GlnHis: 0.743 ± 0.024	1.888GlnIle: 1.888 ± 0.041	1.153GlnLys: 1.153 ± 0.034	2.874GlnLeu: 2.874 ± 0.052	0.811GlnMet: 0.811 ± 0.025	0.975GlnAsn: 0.975 ± 0.028	1.913GlnPro: 1.913 ± 0.046	1.269GlnGln: 1.269 ± 0.04	2.698GlnArg: 2.698 ± 0.052	1.973GlnSer: 1.973 ± 0.039	1.915GlnThr: 1.915 ± 0.039	2.227GlnVal: 2.227 ± 0.05	0.447GlnTrp: 0.447 ± 0.02	0.672GlnTyr: 0.672 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.242ArgAla: 8.242 ± 0.089	0.627ArgCys: 0.627 ± 0.022	4.443ArgAsp: 4.443 ± 0.063	4.187ArgGlu: 4.187 ± 0.067	3.018ArgPhe: 3.018 ± 0.046	5.188ArgGly: 5.188 ± 0.067	1.895ArgHis: 1.895 ± 0.043	4.264ArgIle: 4.264 ± 0.054	2.939ArgLys: 2.939 ± 0.045	7.832ArgLeu: 7.832 ± 0.096	1.828ArgMet: 1.828 ± 0.035	2.143ArgAsn: 2.143 ± 0.037	3.768ArgPro: 3.768 ± 0.067	2.724ArgGln: 2.724 ± 0.043	6.419ArgArg: 6.419 ± 0.092	4.237ArgSer: 4.237 ± 0.062	3.772ArgThr: 3.772 ± 0.059	4.888ArgVal: 4.888 ± 0.059	1.086ArgTrp: 1.086 ± 0.032	1.847ArgTyr: 1.847 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.641SerAla: 6.641 ± 0.07	0.474SerCys: 0.474 ± 0.019	3.413SerAsp: 3.413 ± 0.055	2.992SerGlu: 2.992 ± 0.046	2.301SerPhe: 2.301 ± 0.045	5.723SerGly: 5.723 ± 0.089	1.153SerHis: 1.153 ± 0.029	2.974SerIle: 2.974 ± 0.051	1.987SerLys: 1.987 ± 0.043	5.496SerLeu: 5.496 ± 0.07	1.314SerMet: 1.314 ± 0.032	1.644SerAsn: 1.644 ± 0.043	3.016SerPro: 3.016 ± 0.052	1.776SerGln: 1.776 ± 0.041	4.117SerArg: 4.117 ± 0.057	3.54SerSer: 3.54 ± 0.069	3.002SerThr: 3.002 ± 0.051	4.178SerVal: 4.178 ± 0.058	0.77SerTrp: 0.77 ± 0.029	1.351SerTyr: 1.351 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	6.449ThrAla: 6.449 ± 0.084	0.47ThrCys: 0.47 ± 0.017	2.909ThrAsp: 2.909 ± 0.049	2.532ThrGlu: 2.532 ± 0.044	2.12ThrPhe: 2.12 ± 0.052	4.942ThrGly: 4.942 ± 0.075	0.993ThrHis: 0.993 ± 0.026	3.17ThrIle: 3.17 ± 0.064	1.711ThrLys: 1.711 ± 0.037	5.722ThrLeu: 5.722 ± 0.077	1.25ThrMet: 1.25 ± 0.032	1.425ThrAsn: 1.425 ± 0.044	3.278ThrPro: 3.278 ± 0.053	1.48ThrGln: 1.48 ± 0.038	3.708ThrArg: 3.708 ± 0.056	3.281ThrSer: 3.281 ± 0.059	3.168ThrThr: 3.168 ± 0.068	4.433ThrVal: 4.433 ± 0.067	0.652ThrTrp: 0.652 ± 0.023	1.21ThrTyr: 1.21 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	9.067ValAla: 9.067 ± 0.091	0.635ValCys: 0.635 ± 0.022	4.215ValAsp: 4.215 ± 0.061	4.16ValGlu: 4.16 ± 0.072	2.56ValPhe: 2.56 ± 0.048	5.428ValGly: 5.428 ± 0.071	1.475ValHis: 1.475 ± 0.036	4.078ValIle: 4.078 ± 0.056	2.659ValLys: 2.659 ± 0.052	7.107ValLeu: 7.107 ± 0.085	1.735ValMet: 1.735 ± 0.035	2.049ValAsn: 2.049 ± 0.045	3.645ValPro: 3.645 ± 0.055	2.04ValGln: 2.04 ± 0.045	5.36ValArg: 5.36 ± 0.084	4.448ValSer: 4.448 ± 0.062	4.447ValThr: 4.447 ± 0.062	6.125ValVal: 6.125 ± 0.081	0.917ValTrp: 0.917 ± 0.032	1.478ValTyr: 1.478 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.091TrpAla: 1.091 ± 0.031	0.156TrpCys: 0.156 ± 0.012	0.555TrpAsp: 0.555 ± 0.022	0.486TrpGlu: 0.486 ± 0.019	0.519TrpPhe: 0.519 ± 0.019	0.806TrpGly: 0.806 ± 0.03	0.329TrpHis: 0.329 ± 0.017	0.747TrpIle: 0.747 ± 0.025	0.482TrpLys: 0.482 ± 0.022	1.632TrpLeu: 1.632 ± 0.039	0.343TrpMet: 0.343 ± 0.017	0.494TrpAsn: 0.494 ± 0.021	0.728TrpPro: 0.728 ± 0.023	0.587TrpGln: 0.587 ± 0.024	1.277TrpArg: 1.277 ± 0.031	0.897TrpSer: 0.897 ± 0.028	0.805TrpThr: 0.805 ± 0.029	0.758TrpVal: 0.758 ± 0.028	0.218TrpTrp: 0.218 ± 0.012	0.297TrpTyr: 0.297 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.42TyrAla: 2.42 ± 0.052	0.255TyrCys: 0.255 ± 0.014	1.526TyrAsp: 1.526 ± 0.038	1.107TyrGlu: 1.107 ± 0.03	0.887TyrPhe: 0.887 ± 0.028	2.051TyrGly: 2.051 ± 0.048	0.449TyrHis: 0.449 ± 0.018	0.891TyrIle: 0.891 ± 0.028	0.689TyrLys: 0.689 ± 0.025	2.178TyrLeu: 2.178 ± 0.038	0.405TyrMet: 0.405 ± 0.018	0.662TyrAsn: 0.662 ± 0.025	1.095TyrPro: 1.095 ± 0.029	0.75TyrGln: 0.75 ± 0.023	1.922TyrArg: 1.922 ± 0.04	1.154TyrSer: 1.154 ± 0.035	1.019TyrThr: 1.019 ± 0.032	1.674TyrVal: 1.674 ± 0.042	0.354TyrTrp: 0.354 ± 0.019	0.625TyrTyr: 0.625 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4243 proteins (1297934 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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