Reinekea blandensis MED297

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Saccharospirillaceae; Reinekea; Reinekea blandensis

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4BKL8|A4BKL8_9GAMM Apolipoprotein N-acyltransferase OS=Reinekea blandensis MED297 OX=314283 GN=lnt PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.65KK3 pKa = 10.45LMTIAGVAIALTACGPEE20 pKa = 4.35GSSSYY25 pKa = 11.6DD26 pKa = 3.12LTFKK30 pKa = 9.75ITEE33 pKa = 4.13ANAKK37 pKa = 9.66NVIANSVTLTEE48 pKa = 4.73DD49 pKa = 3.1TDD51 pKa = 3.76EE52 pKa = 4.21YY53 pKa = 10.6TPSKK57 pKa = 10.2MLEE60 pKa = 4.35SYY62 pKa = 10.32SAPTTAIQSRR72 pKa = 11.84ASQSVTLDD80 pKa = 3.57CSPSGTMTLSATVPDD95 pKa = 4.67SYY97 pKa = 11.77ANTGDD102 pKa = 4.41LPTSGQMSVSTTFNNCDD119 pKa = 4.2DD120 pKa = 3.77GWEE123 pKa = 4.21VTDD126 pKa = 4.12GGMSVSMSWSGYY138 pKa = 8.99NEE140 pKa = 4.44SNDD143 pKa = 3.73TYY145 pKa = 11.68NSLTVGIDD153 pKa = 3.27FDD155 pKa = 4.43DD156 pKa = 5.2FKK158 pKa = 11.29VTADD162 pKa = 3.95GEE164 pKa = 4.52TSKK167 pKa = 10.69IDD169 pKa = 3.35GGLDD173 pKa = 2.76ISTGSTSQSVSWDD186 pKa = 3.37LYY188 pKa = 10.22SQFNGQTLRR197 pKa = 11.84TSTPQAISIGLNDD210 pKa = 4.58TYY212 pKa = 11.03PSAGVIRR219 pKa = 11.84VEE221 pKa = 4.44GADD224 pKa = 3.52DD225 pKa = 3.55TFAEE229 pKa = 4.46AKK231 pKa = 9.63IVPNGYY237 pKa = 9.37EE238 pKa = 3.87VTVNGGQATLYY249 pKa = 9.78TWDD252 pKa = 3.33QFF254 pKa = 3.96

Molecular weight: 26.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4BDB7|A4BDB7_9GAMM Uncharacterized protein OS=Reinekea blandensis MED297 OX=314283 GN=MED297_05914 PE=4 SV=1
MM1 pKa = 7.81LFQLSNLKK9 pKa = 10.32EE10 pKa = 3.69IGIMKK15 pKa = 9.22RR16 pKa = 11.84TFQPSNLKK24 pKa = 10.14RR25 pKa = 11.84KK26 pKa = 8.92RR27 pKa = 11.84SHH29 pKa = 6.09GFRR32 pKa = 11.84SRR34 pKa = 11.84MATKK38 pKa = 10.46NGRR41 pKa = 11.84KK42 pKa = 8.96VLARR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84ARR50 pKa = 11.84GRR52 pKa = 11.84ASLSAA57 pKa = 3.92

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4236

0

4236

1345410

21

2587

317.6

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.18 ± 0.036

0.89 ± 0.013

6.154 ± 0.033

6.124 ± 0.035

4.033 ± 0.022

6.957 ± 0.038

2.302 ± 0.021

5.57 ± 0.033

3.678 ± 0.035

10.778 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.52 ± 0.018

3.663 ± 0.024

4.31 ± 0.024

4.834 ± 0.034

5.591 ± 0.033

6.388 ± 0.035

5.635 ± 0.031

7.085 ± 0.028

1.479 ± 0.016

2.829 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski