Amino acid dipepetide frequency for Bacillus sp. FJAT-27916

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.897AlaAla: 6.897 ± 0.105	0.657AlaCys: 0.657 ± 0.026	3.84AlaAsp: 3.84 ± 0.063	5.364AlaGlu: 5.364 ± 0.075	3.343AlaPhe: 3.343 ± 0.069	6.088AlaGly: 6.088 ± 0.088	1.349AlaHis: 1.349 ± 0.034	6.162AlaIle: 6.162 ± 0.084	4.839AlaLys: 4.839 ± 0.085	7.357AlaLeu: 7.357 ± 0.098	2.261AlaMet: 2.261 ± 0.057	2.836AlaAsn: 2.836 ± 0.055	2.162AlaPro: 2.162 ± 0.056	2.221AlaGln: 2.221 ± 0.051	2.953AlaArg: 2.953 ± 0.051	4.357AlaSer: 4.357 ± 0.072	3.093AlaThr: 3.093 ± 0.063	5.865AlaVal: 5.865 ± 0.077	0.609AlaTrp: 0.609 ± 0.024	2.581AlaTyr: 2.581 ± 0.061	0.002AlaXaa: 0.002 ± 0.001
Cys	0.475CysAla: 0.475 ± 0.022	0.084CysCys: 0.084 ± 0.008	0.362CysAsp: 0.362 ± 0.018	0.498CysGlu: 0.498 ± 0.023	0.336CysPhe: 0.336 ± 0.017	0.71CysGly: 0.71 ± 0.027	0.213CysHis: 0.213 ± 0.014	0.55CysIle: 0.55 ± 0.025	0.357CysLys: 0.357 ± 0.02	0.82CysLeu: 0.82 ± 0.031	0.22CysMet: 0.22 ± 0.015	0.249CysAsn: 0.249 ± 0.015	0.385CysPro: 0.385 ± 0.022	0.266CysGln: 0.266 ± 0.016	0.335CysArg: 0.335 ± 0.019	0.493CysSer: 0.493 ± 0.021	0.385CysThr: 0.385 ± 0.02	0.43CysVal: 0.43 ± 0.018	0.066CysTrp: 0.066 ± 0.008	0.262CysTyr: 0.262 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.423AspAla: 3.423 ± 0.06	0.445AspCys: 0.445 ± 0.02	2.469AspAsp: 2.469 ± 0.05	4.715AspGlu: 4.715 ± 0.071	2.393AspPhe: 2.393 ± 0.052	3.642AspGly: 3.642 ± 0.062	1.133AspHis: 1.133 ± 0.033	4.099AspIle: 4.099 ± 0.067	3.218AspLys: 3.218 ± 0.067	4.928AspLeu: 4.928 ± 0.067	1.484AspMet: 1.484 ± 0.036	1.68AspAsn: 1.68 ± 0.041	1.827AspPro: 1.827 ± 0.04	1.798AspGln: 1.798 ± 0.041	2.333AspArg: 2.333 ± 0.047	2.749AspSer: 2.749 ± 0.06	2.481AspThr: 2.481 ± 0.049	3.622AspVal: 3.622 ± 0.056	0.666AspTrp: 0.666 ± 0.023	2.335AspTyr: 2.335 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.887GluAla: 5.887 ± 0.087	0.447GluCys: 0.447 ± 0.02	4.044GluAsp: 4.044 ± 0.07	7.752GluGlu: 7.752 ± 0.111	2.567GluPhe: 2.567 ± 0.053	4.91GluGly: 4.91 ± 0.082	1.495GluHis: 1.495 ± 0.035	5.519GluIle: 5.519 ± 0.072	6.387GluLys: 6.387 ± 0.082	7.346GluLeu: 7.346 ± 0.098	2.521GluMet: 2.521 ± 0.05	3.26GluAsn: 3.26 ± 0.061	2.109GluPro: 2.109 ± 0.045	3.22GluGln: 3.22 ± 0.063	4.029GluArg: 4.029 ± 0.063	3.66GluSer: 3.66 ± 0.055	3.901GluThr: 3.901 ± 0.074	4.883GluVal: 4.883 ± 0.081	0.924GluTrp: 0.924 ± 0.03	2.506GluTyr: 2.506 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.072PheAla: 3.072 ± 0.059	0.33PheCys: 0.33 ± 0.017	2.259PheAsp: 2.259 ± 0.041	2.802PheGlu: 2.802 ± 0.055	2.279PhePhe: 2.279 ± 0.053	3.405PheGly: 3.405 ± 0.064	0.979PheHis: 0.979 ± 0.033	3.789PheIle: 3.789 ± 0.067	2.339PheLys: 2.339 ± 0.05	4.473PheLeu: 4.473 ± 0.095	1.308PheMet: 1.308 ± 0.038	1.731PheAsn: 1.731 ± 0.047	1.583PhePro: 1.583 ± 0.038	1.338PheGln: 1.338 ± 0.035	1.514PheArg: 1.514 ± 0.04	2.948PheSer: 2.948 ± 0.052	2.583PheThr: 2.583 ± 0.053	2.845PheVal: 2.845 ± 0.054	0.484PheTrp: 0.484 ± 0.023	1.706PheTyr: 1.706 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.212GlyAla: 5.212 ± 0.09	0.642GlyCys: 0.642 ± 0.029	3.291GlyAsp: 3.291 ± 0.067	4.95GlyGlu: 4.95 ± 0.074	3.396GlyPhe: 3.396 ± 0.061	5.155GlyGly: 5.155 ± 0.092	1.516GlyHis: 1.516 ± 0.042	6.216GlyIle: 6.216 ± 0.078	5.171GlyLys: 5.171 ± 0.085	6.994GlyLeu: 6.994 ± 0.092	2.348GlyMet: 2.348 ± 0.052	2.619GlyAsn: 2.619 ± 0.049	1.83GlyPro: 1.83 ± 0.057	2.393GlyGln: 2.393 ± 0.053	3.035GlyArg: 3.035 ± 0.053	4.084GlySer: 4.084 ± 0.075	4.238GlyThr: 4.238 ± 0.075	5.078GlyVal: 5.078 ± 0.081	0.861GlyTrp: 0.861 ± 0.032	3.037GlyTyr: 3.037 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.391HisAla: 1.391 ± 0.043	0.194HisCys: 0.194 ± 0.012	1.051HisAsp: 1.051 ± 0.033	1.446HisGlu: 1.446 ± 0.039	1.018HisPhe: 1.018 ± 0.028	1.406HisGly: 1.406 ± 0.036	0.661HisHis: 0.661 ± 0.026	1.557HisIle: 1.557 ± 0.033	1.094HisLys: 1.094 ± 0.035	2.044HisLeu: 2.044 ± 0.04	0.58HisMet: 0.58 ± 0.025	0.755HisAsn: 0.755 ± 0.027	1.13HisPro: 1.13 ± 0.035	0.736HisGln: 0.736 ± 0.024	0.903HisArg: 0.903 ± 0.029	1.228HisSer: 1.228 ± 0.037	1.079HisThr: 1.079 ± 0.028	1.32HisVal: 1.32 ± 0.037	0.188HisTrp: 0.188 ± 0.014	0.936HisTyr: 0.936 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.189IleAla: 6.189 ± 0.086	0.654IleCys: 0.654 ± 0.025	4.2IleAsp: 4.2 ± 0.059	5.582IleGlu: 5.582 ± 0.08	3.117IlePhe: 3.117 ± 0.064	6.216IleGly: 6.216 ± 0.089	1.774IleHis: 1.774 ± 0.043	6.13IleIle: 6.13 ± 0.104	4.477IleLys: 4.477 ± 0.071	7.434IleLeu: 7.434 ± 0.096	2.087IleMet: 2.087 ± 0.047	2.999IleAsn: 2.999 ± 0.057	3.405IlePro: 3.405 ± 0.059	2.924IleGln: 2.924 ± 0.06	3.216IleArg: 3.216 ± 0.058	4.861IleSer: 4.861 ± 0.073	4.313IleThr: 4.313 ± 0.059	5.115IleVal: 5.115 ± 0.085	0.706IleTrp: 0.706 ± 0.029	2.5IleTyr: 2.5 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	5.219LysAla: 5.219 ± 0.079	0.338LysCys: 0.338 ± 0.018	3.746LysAsp: 3.746 ± 0.064	7.138LysGlu: 7.138 ± 0.094	1.635LysPhe: 1.635 ± 0.041	4.858LysGly: 4.858 ± 0.071	1.189LysHis: 1.189 ± 0.035	4.129LysIle: 4.129 ± 0.079	5.65LysLys: 5.65 ± 0.087	5.388LysLeu: 5.388 ± 0.08	2.112LysMet: 2.112 ± 0.044	2.904LysAsn: 2.904 ± 0.056	2.399LysPro: 2.399 ± 0.048	2.84LysGln: 2.84 ± 0.06	3.49LysArg: 3.49 ± 0.057	3.348LysSer: 3.348 ± 0.058	3.607LysThr: 3.607 ± 0.071	4.451LysVal: 4.451 ± 0.078	0.855LysTrp: 0.855 ± 0.029	2.086LysTyr: 2.086 ± 0.043	0.001LysXaa: 0.001 ± 0.001
Leu	7.806LeuAla: 7.806 ± 0.106	0.744LeuCys: 0.744 ± 0.026	4.906LeuAsp: 4.906 ± 0.07	6.658LeuGlu: 6.658 ± 0.082	4.808LeuPhe: 4.808 ± 0.091	6.544LeuGly: 6.544 ± 0.077	1.896LeuHis: 1.896 ± 0.043	7.336LeuIle: 7.336 ± 0.115	6.23LeuLys: 6.23 ± 0.093	10.11LeuLeu: 10.11 ± 0.152	2.746LeuMet: 2.746 ± 0.05	3.862LeuAsn: 3.862 ± 0.061	3.958LeuPro: 3.958 ± 0.069	3.269LeuGln: 3.269 ± 0.051	3.675LeuArg: 3.675 ± 0.052	6.905LeuSer: 6.905 ± 0.09	5.504LeuThr: 5.504 ± 0.076	6.017LeuVal: 6.017 ± 0.084	0.831LeuTrp: 0.831 ± 0.034	3.355LeuTyr: 3.355 ± 0.059	0.001LeuXaa: 0.001 ± 0.001
Met	2.405MetAla: 2.405 ± 0.045	0.157MetCys: 0.157 ± 0.013	1.747MetAsp: 1.747 ± 0.043	2.281MetGlu: 2.281 ± 0.049	1.035MetPhe: 1.035 ± 0.03	1.971MetGly: 1.971 ± 0.042	0.467MetHis: 0.467 ± 0.018	2.266MetIle: 2.266 ± 0.041	2.658MetLys: 2.658 ± 0.044	2.656MetLeu: 2.656 ± 0.056	0.97MetMet: 0.97 ± 0.034	1.705MetAsn: 1.705 ± 0.039	1.128MetPro: 1.128 ± 0.036	0.92MetGln: 0.92 ± 0.026	1.238MetArg: 1.238 ± 0.037	1.684MetSer: 1.684 ± 0.042	1.776MetThr: 1.776 ± 0.049	1.898MetVal: 1.898 ± 0.041	0.161MetTrp: 0.161 ± 0.013	0.783MetTyr: 0.783 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.708AsnAla: 2.708 ± 0.048	0.283AsnCys: 0.283 ± 0.016	2.082AsnAsp: 2.082 ± 0.043	3.223AsnGlu: 3.223 ± 0.057	1.379AsnPhe: 1.379 ± 0.037	3.421AsnGly: 3.421 ± 0.057	1.055AsnHis: 1.055 ± 0.032	2.985AsnIle: 2.985 ± 0.058	2.519AsnLys: 2.519 ± 0.052	3.517AsnLeu: 3.517 ± 0.061	1.149AsnMet: 1.149 ± 0.034	1.492AsnAsn: 1.492 ± 0.046	2.116AsnPro: 2.116 ± 0.047	1.852AsnGln: 1.852 ± 0.046	2.019AsnArg: 2.019 ± 0.046	1.997AsnSer: 1.997 ± 0.043	1.986AsnThr: 1.986 ± 0.045	2.613AsnVal: 2.613 ± 0.051	0.497AsnTrp: 0.497 ± 0.022	1.337AsnTyr: 1.337 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.663ProAla: 2.663 ± 0.05	0.245ProCys: 0.245 ± 0.015	2.174ProAsp: 2.174 ± 0.047	3.046ProGlu: 3.046 ± 0.048	1.987ProPhe: 1.987 ± 0.044	2.412ProGly: 2.412 ± 0.056	0.752ProHis: 0.752 ± 0.03	2.791ProIle: 2.791 ± 0.044	2.12ProLys: 2.12 ± 0.047	3.512ProLeu: 3.512 ± 0.055	0.881ProMet: 0.881 ± 0.028	1.507ProAsn: 1.507 ± 0.039	0.988ProPro: 0.988 ± 0.036	0.992ProGln: 0.992 ± 0.03	1.157ProArg: 1.157 ± 0.04	2.31ProSer: 2.31 ± 0.047	1.778ProThr: 1.778 ± 0.057	2.87ProVal: 2.87 ± 0.054	0.333ProTrp: 0.333 ± 0.019	1.52ProTyr: 1.52 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.966GlnAla: 2.966 ± 0.065	0.211GlnCys: 0.211 ± 0.015	1.615GlnAsp: 1.615 ± 0.043	2.777GlnGlu: 2.777 ± 0.053	1.45GlnPhe: 1.45 ± 0.04	2.092GlnGly: 2.092 ± 0.042	0.667GlnHis: 0.667 ± 0.025	2.465GlnIle: 2.465 ± 0.05	2.524GlnLys: 2.524 ± 0.053	3.729GlnLeu: 3.729 ± 0.063	1.194GlnMet: 1.194 ± 0.032	1.424GlnAsn: 1.424 ± 0.034	1.057GlnPro: 1.057 ± 0.034	1.34GlnGln: 1.34 ± 0.036	1.474GlnArg: 1.474 ± 0.042	2.075GlnSer: 2.075 ± 0.052	1.867GlnThr: 1.867 ± 0.039	2.237GlnVal: 2.237 ± 0.049	0.377GlnTrp: 0.377 ± 0.019	1.411GlnTyr: 1.411 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.622ArgAla: 2.622 ± 0.048	0.265ArgCys: 0.265 ± 0.017	2.075ArgAsp: 2.075 ± 0.044	3.55ArgGlu: 3.55 ± 0.063	1.977ArgPhe: 1.977 ± 0.049	2.447ArgGly: 2.447 ± 0.05	0.869ArgHis: 0.869 ± 0.031	3.407ArgIle: 3.407 ± 0.064	3.409ArgLys: 3.409 ± 0.06	4.362ArgLeu: 4.362 ± 0.074	1.515ArgMet: 1.515 ± 0.036	1.902ArgAsn: 1.902 ± 0.041	1.343ArgPro: 1.343 ± 0.033	1.637ArgGln: 1.637 ± 0.041	1.982ArgArg: 1.982 ± 0.042	2.222ArgSer: 2.222 ± 0.044	2.192ArgThr: 2.192 ± 0.046	2.652ArgVal: 2.652 ± 0.051	0.415ArgTrp: 0.415 ± 0.02	1.698ArgTyr: 1.698 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.981SerAla: 3.981 ± 0.075	0.435SerCys: 0.435 ± 0.022	2.754SerAsp: 2.754 ± 0.052	3.864SerGlu: 3.864 ± 0.064	3.215SerPhe: 3.215 ± 0.056	4.627SerGly: 4.627 ± 0.073	1.217SerHis: 1.217 ± 0.034	5.051SerIle: 5.051 ± 0.07	3.574SerLys: 3.574 ± 0.059	6.046SerLeu: 6.046 ± 0.083	1.899SerMet: 1.899 ± 0.043	2.185SerAsn: 2.185 ± 0.047	2.185SerPro: 2.185 ± 0.047	1.762SerGln: 1.762 ± 0.037	2.471SerArg: 2.471 ± 0.05	4.031SerSer: 4.031 ± 0.087	2.91SerThr: 2.91 ± 0.052	3.961SerVal: 3.961 ± 0.065	0.658SerTrp: 0.658 ± 0.026	2.351SerTyr: 2.351 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	4.302ThrAla: 4.302 ± 0.066	0.341ThrCys: 0.341 ± 0.018	2.806ThrAsp: 2.806 ± 0.048	3.507ThrGlu: 3.507 ± 0.063	2.472ThrPhe: 2.472 ± 0.059	4.432ThrGly: 4.432 ± 0.097	1.021ThrHis: 1.021 ± 0.032	4.509ThrIle: 4.509 ± 0.065	3.271ThrLys: 3.271 ± 0.064	4.982ThrLeu: 4.982 ± 0.075	1.304ThrMet: 1.304 ± 0.039	2.297ThrAsn: 2.297 ± 0.046	2.152ThrPro: 2.152 ± 0.051	1.276ThrGln: 1.276 ± 0.036	1.841ThrArg: 1.841 ± 0.039	3.071ThrSer: 3.071 ± 0.059	2.672ThrThr: 2.672 ± 0.058	4.073ThrVal: 4.073 ± 0.066	0.517ThrTrp: 0.517 ± 0.023	1.972ThrTyr: 1.972 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.579ValAla: 4.579 ± 0.09	0.598ValCys: 0.598 ± 0.024	3.474ValAsp: 3.474 ± 0.07	4.66ValGlu: 4.66 ± 0.078	3.089ValPhe: 3.089 ± 0.063	4.309ValGly: 4.309 ± 0.075	1.358ValHis: 1.358 ± 0.037	5.633ValIle: 5.633 ± 0.081	4.649ValLys: 4.649 ± 0.063	6.731ValLeu: 6.731 ± 0.086	1.98ValMet: 1.98 ± 0.049	2.962ValAsn: 2.962 ± 0.056	2.553ValPro: 2.553 ± 0.047	2.336ValGln: 2.336 ± 0.049	2.741ValArg: 2.741 ± 0.049	4.366ValSer: 4.366 ± 0.064	3.921ValThr: 3.921 ± 0.059	4.449ValVal: 4.449 ± 0.074	0.644ValTrp: 0.644 ± 0.024	2.299ValTyr: 2.299 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.678TrpAla: 0.678 ± 0.026	0.084TrpCys: 0.084 ± 0.009	0.526TrpAsp: 0.526 ± 0.024	0.728TrpGlu: 0.728 ± 0.026	0.489TrpPhe: 0.489 ± 0.02	0.722TrpGly: 0.722 ± 0.028	0.219TrpHis: 0.219 ± 0.013	0.805TrpIle: 0.805 ± 0.028	0.722TrpLys: 0.722 ± 0.026	1.094TrpLeu: 1.094 ± 0.031	0.369TrpMet: 0.369 ± 0.019	0.547TrpAsn: 0.547 ± 0.023	0.26TrpPro: 0.26 ± 0.015	0.339TrpGln: 0.339 ± 0.017	0.427TrpArg: 0.427 ± 0.02	0.603TrpSer: 0.603 ± 0.025	0.554TrpThr: 0.554 ± 0.025	0.691TrpVal: 0.691 ± 0.027	0.127TrpTrp: 0.127 ± 0.011	0.341TrpTyr: 0.341 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.361TyrAla: 2.361 ± 0.045	0.342TyrCys: 0.342 ± 0.018	2.022TyrAsp: 2.022 ± 0.046	2.827TyrGlu: 2.827 ± 0.062	1.781TyrPhe: 1.781 ± 0.044	2.696TyrGly: 2.696 ± 0.05	0.887TyrHis: 0.887 ± 0.027	2.5TyrIle: 2.5 ± 0.044	2.173TyrLys: 2.173 ± 0.051	3.635TyrLeu: 3.635 ± 0.068	0.978TyrMet: 0.978 ± 0.031	1.313TyrAsn: 1.313 ± 0.037	1.469TyrPro: 1.469 ± 0.037	1.492TyrGln: 1.492 ± 0.038	1.71TyrArg: 1.71 ± 0.042	2.182TyrSer: 2.182 ± 0.046	1.978TyrThr: 1.978 ± 0.05	2.263TyrVal: 2.263 ± 0.045	0.407TyrTrp: 0.407 ± 0.019	1.517TyrTyr: 1.517 ± 0.046	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.013XaaXaa: 0.013 ± 0.006

Statistics based on 3770 proteins (1089385 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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