Oblitimonas alkaliphila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Oblitimonas

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1XDB9|A0A0K1XDB9_9GAMM 50S ribosomal protein L28 OS=Oblitimonas alkaliphila OX=1697053 GN=rpmB PE=3 SV=1
MM1 pKa = 7.6SAQQSAYY8 pKa = 10.53HH9 pKa = 6.32SFATLLTSLQQPVTPAEE26 pKa = 4.03LQGFLLGRR34 pKa = 11.84SCANANFDD42 pKa = 3.69QQQWLLDD49 pKa = 3.74AAEE52 pKa = 4.5LFAGEE57 pKa = 4.53LPEE60 pKa = 4.38TLHH63 pKa = 7.1SALQGLQLMTYY74 pKa = 10.02QEE76 pKa = 4.25LTNTEE81 pKa = 3.99TMALTLLLPEE91 pKa = 5.35DD92 pKa = 4.17EE93 pKa = 5.15LSLSEE98 pKa = 4.28RR99 pKa = 11.84LQALSEE105 pKa = 4.07WCQSFLAGFGLSIGSIVVTEE125 pKa = 4.0QAAEE129 pKa = 3.99VLEE132 pKa = 4.8DD133 pKa = 4.99FVALANIADD142 pKa = 4.11AEE144 pKa = 4.33DD145 pKa = 4.38GEE147 pKa = 5.01EE148 pKa = 4.0NEE150 pKa = 3.84QDD152 pKa = 3.54YY153 pKa = 10.62MEE155 pKa = 4.31VSEE158 pKa = 4.48YY159 pKa = 11.1LRR161 pKa = 11.84VAPLLLFAEE170 pKa = 5.35CGAKK174 pKa = 8.05QTIVAPQDD182 pKa = 3.43RR183 pKa = 11.84APVTHH188 pKa = 7.26

Molecular weight: 20.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1XEM7|A0A0K1XEM7_9GAMM Aspartate ammonia-lyase OS=Oblitimonas alkaliphila OX=1697053 GN=aspA PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.47RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 5.31GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11SGRR28 pKa = 11.84AILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2127

0

2127

673168

36

1820

316.5

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.366 ± 0.059

0.981 ± 0.016

4.734 ± 0.042

6.078 ± 0.058

3.765 ± 0.033

6.979 ± 0.051

2.231 ± 0.025

5.534 ± 0.038

4.307 ± 0.044

11.838 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.026

3.393 ± 0.035

4.412 ± 0.03

5.97 ± 0.077

5.216 ± 0.045

5.845 ± 0.038

5.022 ± 0.028

6.87 ± 0.047

1.363 ± 0.025

2.766 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski