Xingshan nematode virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFD6|A0A1L3KFD6_9VIRU RdRp catalytic domain-containing protein OS=Xingshan nematode virus 6 OX=1923765 PE=4 SV=1
MM1 pKa = 7.66ASDD4 pKa = 3.96SLKK7 pKa = 9.56STDD10 pKa = 3.28YY11 pKa = 10.98VGRR14 pKa = 11.84LRR16 pKa = 11.84SALGNRR22 pKa = 11.84RR23 pKa = 11.84TVQDD27 pKa = 3.85RR28 pKa = 11.84EE29 pKa = 4.41TEE31 pKa = 4.17DD32 pKa = 3.3FTVASGLAFYY42 pKa = 10.64SALFTPVATSILPGDD57 pKa = 3.96VRR59 pKa = 11.84EE60 pKa = 4.56AMCGLNTVSQKK71 pKa = 9.82TSGSGCFSLHH81 pKa = 5.83HH82 pKa = 5.96TVSDD86 pKa = 3.52IMPLNLEE93 pKa = 4.74LGDD96 pKa = 3.76EE97 pKa = 4.36KK98 pKa = 11.05KK99 pKa = 11.18GNNRR103 pKa = 11.84AARR106 pKa = 11.84KK107 pKa = 9.7VSGGNGKK114 pKa = 8.67VKK116 pKa = 10.41RR117 pKa = 11.84KK118 pKa = 10.58SNDD121 pKa = 3.07QGWDD125 pKa = 3.25VKK127 pKa = 10.84NQAVHH132 pKa = 5.11GRR134 pKa = 11.84EE135 pKa = 4.2VKK137 pKa = 10.52VSTGLGCHH145 pKa = 5.75QGTPPVTSLYY155 pKa = 10.03AQLVHH160 pKa = 6.42RR161 pKa = 11.84QCLPLEE167 pKa = 4.67AGSGTQASTTNNPVYY182 pKa = 10.31TDD184 pKa = 3.66YY185 pKa = 11.72GSLAFMTEE193 pKa = 4.13VVTGQPGSWKK203 pKa = 10.32DD204 pKa = 3.34DD205 pKa = 3.62APILRR210 pKa = 11.84MLLLGDD216 pKa = 4.03IFAMADD222 pKa = 3.64RR223 pKa = 11.84YY224 pKa = 10.9KK225 pKa = 10.8LDD227 pKa = 4.91PIWYY231 pKa = 9.54KK232 pKa = 10.41LARR235 pKa = 11.84DD236 pKa = 4.19ANPSSIEE243 pKa = 3.51WHH245 pKa = 5.01MTGLPKK251 pKa = 10.67EE252 pKa = 4.34GGVNSRR258 pKa = 11.84SVQITAVTLDD268 pKa = 2.84VWAALRR274 pKa = 11.84VGSLKK279 pKa = 10.9DD280 pKa = 3.35STLSEE285 pKa = 4.14LVQGVNCAVIPISSNKK301 pKa = 9.5LDD303 pKa = 4.12EE304 pKa = 4.3AWIWLYY310 pKa = 10.96LAAFCTSRR318 pKa = 11.84IWNGLHH324 pKa = 4.62QWEE327 pKa = 4.67VSLDD331 pKa = 3.37PSVRR335 pKa = 11.84SKK337 pKa = 11.07SEE339 pKa = 3.81KK340 pKa = 9.97LLEE343 pKa = 4.6TIFTTRR349 pKa = 11.84SSLCMVEE356 pKa = 3.77GHH358 pKa = 5.8EE359 pKa = 5.3RR360 pKa = 11.84ILLVLTDD367 pKa = 3.33VTSRR371 pKa = 11.84LGYY374 pKa = 8.45KK375 pKa = 6.51TTRR378 pKa = 11.84LARR381 pKa = 11.84EE382 pKa = 4.07KK383 pKa = 10.24IVPIWQKK390 pKa = 9.64TDD392 pKa = 3.44PKK394 pKa = 10.29VGWVEE399 pKa = 4.08IIDD402 pKa = 3.82EE403 pKa = 4.24LRR405 pKa = 11.84YY406 pKa = 8.84ATGVTTLDD414 pKa = 3.76YY415 pKa = 11.08PHH417 pKa = 7.33LGDD420 pKa = 4.24LWRR423 pKa = 11.84MAWKK427 pKa = 10.67EE428 pKa = 3.84MMASVATGDD437 pKa = 3.74SVNRR441 pKa = 11.84CMALVAEE448 pKa = 5.16ATRR451 pKa = 11.84VMDD454 pKa = 3.13VGFYY458 pKa = 10.66SGGEE462 pKa = 4.14VNVMSLGKK470 pKa = 9.96IEE472 pKa = 4.89GLPEE476 pKa = 4.68LDD478 pKa = 3.04GVVWKK483 pKa = 10.42NRR485 pKa = 11.84QWEE488 pKa = 4.58SMTADD493 pKa = 3.47DD494 pKa = 6.02AKK496 pKa = 11.02FGLQDD501 pKa = 4.16ASLSWVSVSGMRR513 pKa = 11.84PDD515 pKa = 4.24FAWTVLDD522 pKa = 4.0PEE524 pKa = 5.08KK525 pKa = 10.52GRR527 pKa = 11.84SMLTVGGIDD536 pKa = 3.09ASLYY540 pKa = 9.16CHH542 pKa = 6.67EE543 pKa = 4.82ATGDD547 pKa = 3.34MRR549 pKa = 11.84MARR552 pKa = 11.84YY553 pKa = 9.83VGIISGAEE561 pKa = 3.43RR562 pKa = 11.84VALEE566 pKa = 3.89TCVAVSEE573 pKa = 4.33NVSAIASFMACAVGWMQSVAGIPWYY598 pKa = 10.37KK599 pKa = 9.14WAHH602 pKa = 5.09HH603 pKa = 6.67HH604 pKa = 6.22NLPEE608 pKa = 4.12LTSIDD613 pKa = 4.2DD614 pKa = 4.3LASMMLDD621 pKa = 3.34AASDD625 pKa = 3.57GHH627 pKa = 6.17IKK629 pKa = 10.32RR630 pKa = 11.84SYY632 pKa = 11.29ADD634 pKa = 3.71PTWCTWVEE642 pKa = 3.58NNIFHH647 pKa = 6.27GQPWSKK653 pKa = 10.77VCTTILPWWAMKK665 pKa = 9.7FVIQKK670 pKa = 9.9VGYY673 pKa = 8.62HH674 pKa = 6.53LDD676 pKa = 3.26VGLVEE681 pKa = 4.11RR682 pKa = 11.84QKK684 pKa = 10.59PVEE687 pKa = 4.15GKK689 pKa = 10.17RR690 pKa = 11.84PRR692 pKa = 11.84IGYY695 pKa = 6.04WATMASEE702 pKa = 4.21WQNVARR708 pKa = 11.84VTSRR712 pKa = 11.84ITVRR716 pKa = 11.84YY717 pKa = 8.4PDD719 pKa = 3.68EE720 pKa = 4.18CVEE723 pKa = 4.64YY724 pKa = 9.55YY725 pKa = 10.06TSSYY729 pKa = 11.83DD730 pKa = 3.79NANLGAGVVGYY741 pKa = 9.59NWHH744 pKa = 6.6RR745 pKa = 11.84AAAEE749 pKa = 4.02YY750 pKa = 11.13GEE752 pKa = 4.69SQYY755 pKa = 11.13HH756 pKa = 5.09WQTSARR762 pKa = 11.84PWNKK766 pKa = 10.09SVDD769 pKa = 3.71SSTLITPVSYY779 pKa = 11.4AMVYY783 pKa = 10.22GNPSGMLITMTRR795 pKa = 11.84NRR797 pKa = 11.84VGSGMDD803 pKa = 3.52DD804 pKa = 3.19QDD806 pKa = 3.74VTGRR810 pKa = 11.84WPDD813 pKa = 3.46PWWNWLLEE821 pKa = 4.19GVYY824 pKa = 9.95EE825 pKa = 4.35ALPHH829 pKa = 6.68LLQGNLLGAALAGMSGGVNAFFRR852 pKa = 11.84EE853 pKa = 3.84RR854 pKa = 11.84LNPVSAQKK862 pKa = 9.93EE863 pKa = 3.93QQARR867 pKa = 11.84MEE869 pKa = 4.37SAARR873 pKa = 11.84AADD876 pKa = 4.11KK877 pKa = 10.8ILTAGKK883 pKa = 10.18DD884 pKa = 3.55LVEE887 pKa = 4.66HH888 pKa = 6.45GKK890 pKa = 9.91AAAAEE895 pKa = 4.25TVAAARR901 pKa = 11.84EE902 pKa = 4.24FAGEE906 pKa = 4.23AGPSGPAA913 pKa = 3.07

Molecular weight: 100.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFL3|A0A1L3KFL3_9VIRU Uncharacterized protein OS=Xingshan nematode virus 6 OX=1923765 PE=4 SV=1
MM1 pKa = 7.18FSYY4 pKa = 10.65VRR6 pKa = 11.84RR7 pKa = 11.84GPRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84GTEE16 pKa = 3.55TASAPLVVWGLRR28 pKa = 11.84LIRR31 pKa = 11.84RR32 pKa = 11.84NSIPLAGWGLPGYY45 pKa = 10.39SGVLTRR51 pKa = 11.84NLGTTSSINGGRR63 pKa = 11.84FCMVRR68 pKa = 11.84DD69 pKa = 4.19LRR71 pKa = 11.84GSWLLVVGCKK81 pKa = 9.84QPVKK85 pKa = 9.73QAHH88 pKa = 6.17RR89 pKa = 11.84STWEE93 pKa = 3.64CSGALGVTGNNKK105 pKa = 9.09ARR107 pKa = 11.84RR108 pKa = 11.84VYY110 pKa = 10.86RR111 pKa = 11.84MAGISGRR118 pKa = 11.84IPPRR122 pKa = 11.84PLPLGVPCHH131 pKa = 5.41FVVRR135 pKa = 11.84VHH137 pKa = 6.18GCPWHH142 pKa = 5.83GG143 pKa = 3.35

Molecular weight: 15.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1746

143

913

582.0

65.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.362 ± 1.131

1.947 ± 0.459

5.04 ± 1.112

4.811 ± 0.904

2.176 ± 0.242

8.534 ± 1.403

2.234 ± 0.144

3.608 ± 0.028

4.353 ± 0.6

9.737 ± 1.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.296

2.749 ± 0.715

4.525 ± 0.831

3.036 ± 0.44

7.388 ± 2.015

7.102 ± 0.751

5.613 ± 0.553

8.247 ± 0.495

4.066 ± 0.321

3.265 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski