Clerodendron golden mosaic virus
Average proteome isoelectric point is 8.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5H161|A5H161_9GEMI Nuclear shuttle protein OS=Clerodendron golden mosaic virus OX=390438 GN=BV1 PE=3 SV=1
MM1 pKa = 7.01 LTSHH5 pKa = 7.01 TGLSDD10 pKa = 2.92 MWDD13 pKa = 3.41 PLVNDD18 pKa = 4.5 FPEE21 pKa = 4.69 TVHH24 pKa = 7.1 GLRR27 pKa = 11.84 CMLAVKK33 pKa = 9.32 YY34 pKa = 10.21 LKK36 pKa = 10.48 YY37 pKa = 10.79 VEE39 pKa = 4.49 STYY42 pKa = 11.35 SPDD45 pKa = 3.14 TLGYY49 pKa = 10.84 DD50 pKa = 4.78 LIRR53 pKa = 11.84 DD54 pKa = 4.52 LILVVRR60 pKa = 11.84 AKK62 pKa = 10.88 NYY64 pKa = 9.84 GEE66 pKa = 3.59 ASRR69 pKa = 11.84 RR70 pKa = 11.84 YY71 pKa = 8.44 NHH73 pKa = 6.23 FHH75 pKa = 6.26 TRR77 pKa = 11.84 LQGSTEE83 pKa = 3.94 AEE85 pKa = 3.74 LRR87 pKa = 11.84 QPVSEE92 pKa = 4.49 PCACPHH98 pKa = 6.68 CPRR101 pKa = 11.84 HH102 pKa = 5.62 TKK104 pKa = 9.12 TVMGQSAHH112 pKa = 4.79 VQEE115 pKa = 4.92 AQDD118 pKa = 3.2 II119 pKa = 4.06
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.935
IPC2_protein 5.957
IPC_protein 6.059
Toseland 6.44
ProMoST 6.465
Dawson 6.402
Bjellqvist 6.351
Wikipedia 6.427
Rodwell 6.389
Grimsley 6.59
Solomon 6.402
Lehninger 6.402
Nozaki 6.678
DTASelect 6.868
Thurlkill 6.898
EMBOSS 6.868
Sillero 6.795
Patrickios 3.605
IPC_peptide 6.427
IPC2_peptide 6.781
IPC2.peptide.svr19 6.667
Protein with the highest isoelectric point:
>tr|A5H160|A5H160_9GEMI Protein V2 OS=Clerodendron golden mosaic virus OX=390438 GN=AV2 PE=3 SV=1
MM1 pKa = 7.62 PKK3 pKa = 9.84 IIFSLIHH10 pKa = 6.02 NAHH13 pKa = 5.11 SQKK16 pKa = 10.84 KK17 pKa = 9.76 KK18 pKa = 10.18 LLPDD22 pKa = 3.9 FWQLLHH28 pKa = 6.62 QPIKK32 pKa = 10.15 NSSRR36 pKa = 11.84 FAEE39 pKa = 4.45 NSTKK43 pKa = 9.92 MGALISTFLCNSRR56 pKa = 11.84 ANSNARR62 pKa = 11.84 INDD65 pKa = 3.25 SSIWYY70 pKa = 6.72 PQQDD74 pKa = 3.87 QPTSILTFKK83 pKa = 10.22 GQNRR87 pKa = 11.84 VPTSSPIWIRR97 pKa = 11.84 TGTHH101 pKa = 4.79 WNGEE105 pKa = 4.27 NSRR108 pKa = 11.84 STGDD112 pKa = 3.23 LHH114 pKa = 8.01 EE115 pKa = 4.55 EE116 pKa = 3.92 VHH118 pKa = 6.05 RR119 pKa = 11.84 QLMMLTPRR127 pKa = 11.84 RR128 pKa = 11.84 LTLAVSTKK136 pKa = 10.53 LLRR139 pKa = 11.84 SLKK142 pKa = 10.43 NN143 pKa = 3.27
Molecular weight: 16.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.706
IPC_protein 10.321
Toseland 11.023
ProMoST 10.687
Dawson 11.067
Bjellqvist 10.745
Wikipedia 11.257
Rodwell 11.359
Grimsley 11.082
Solomon 11.228
Lehninger 11.199
Nozaki 10.994
DTASelect 10.745
Thurlkill 10.994
EMBOSS 11.418
Sillero 10.994
Patrickios 11.111
IPC_peptide 11.242
IPC2_peptide 9.545
IPC2.peptide.svr19 8.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1700
119
355
212.5
24.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.176 ± 0.504
1.882 ± 0.208
4.647 ± 0.496
4.0 ± 0.479
4.294 ± 0.406
4.941 ± 0.243
3.647 ± 0.825
5.941 ± 0.719
5.059 ± 0.602
7.412 ± 0.726
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.824 ± 0.231
5.824 ± 0.605
5.529 ± 0.337
4.353 ± 0.476
7.529 ± 0.952
9.059 ± 1.188
6.765 ± 0.335
6.529 ± 0.934
1.294 ± 0.186
4.294 ± 0.698
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here