Candidatus Pseudomonas adelgestsugas

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481QLJ7|A0A481QLJ7_9PSED Adenosylhomocysteinase OS=Candidatus Pseudomonas adelgestsugas OX=1302376 GN=ahcY PE=3 SV=1
MM1 pKa = 7.38QSLLVISRR9 pKa = 11.84QAPWSGLGALDD20 pKa = 4.6ALDD23 pKa = 3.46IVLAGGVFDD32 pKa = 6.02LPIGLLFMDD41 pKa = 5.23DD42 pKa = 4.69GVFQLAPDD50 pKa = 3.56QNAAAVQQRR59 pKa = 11.84DD60 pKa = 3.35LCANLQVLNLFGIDD74 pKa = 4.17DD75 pKa = 4.15IFVCSHH81 pKa = 6.14SLAEE85 pKa = 4.23RR86 pKa = 11.84QLVSPTSVQVLSNNAIYY103 pKa = 10.46QLIDD107 pKa = 3.25QYY109 pKa = 11.81NQIITII115 pKa = 4.16

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481QNB7|A0A481QNB7_9PSED Glycosyltransferase EpsE OS=Candidatus Pseudomonas adelgestsugas OX=1302376 GN=epsE PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.05RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.99NGRR28 pKa = 11.84AILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.62GRR39 pKa = 11.84IRR41 pKa = 11.84LAVV44 pKa = 3.28

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

944

0

944

275091

29

1622

291.4

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.708 ± 0.077

1.342 ± 0.03

4.964 ± 0.059

5.304 ± 0.064

3.893 ± 0.052

6.898 ± 0.068

2.405 ± 0.033

7.237 ± 0.066

5.148 ± 0.062

11.144 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.032

4.264 ± 0.059

3.825 ± 0.043

3.927 ± 0.053

5.437 ± 0.076

6.231 ± 0.059

5.296 ± 0.063

7.147 ± 0.067

1.151 ± 0.035

3.047 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski