Klebsiella phage ST13-OXA48phi12.3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J308|A0A482J308_9CAUD Uncharacterized protein OS=Klebsiella phage ST13-OXA48phi12.3 OX=2510466 PE=4 SV=1
MM1 pKa = 7.31YY2 pKa = 10.23KK3 pKa = 10.13HH4 pKa = 6.47LNISITLCGEE14 pKa = 4.05EE15 pKa = 4.24TCTDD19 pKa = 3.61EE20 pKa = 5.43LQISLDD26 pKa = 3.76DD27 pKa = 4.39VIRR30 pKa = 11.84SADD33 pKa = 3.24VAGRR37 pKa = 11.84IGTLIGEE44 pKa = 5.24GYY46 pKa = 10.23RR47 pKa = 11.84EE48 pKa = 4.22GTFDD52 pKa = 5.28FHH54 pKa = 9.49IEE56 pKa = 3.93DD57 pKa = 3.69NAMSVAWNCTTSEE70 pKa = 4.06SRR72 pKa = 3.69

Molecular weight: 8.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J551|A0A482J551_9CAUD Uncharacterized protein OS=Klebsiella phage ST13-OXA48phi12.3 OX=2510466 PE=4 SV=1
MM1 pKa = 7.23TSSRR5 pKa = 11.84NNFWDD10 pKa = 5.54FIQMIKK16 pKa = 10.33RR17 pKa = 11.84LEE19 pKa = 4.12SGKK22 pKa = 9.58PVLFQKK28 pKa = 10.39PYY30 pKa = 9.27PPEE33 pKa = 4.27GNPQAFYY40 pKa = 10.89LGQLTKK46 pKa = 10.65RR47 pKa = 11.84GLLSRR52 pKa = 11.84NSFPAHH58 pKa = 4.87TEE60 pKa = 3.4YY61 pKa = 11.17RR62 pKa = 11.84LRR64 pKa = 11.84KK65 pKa = 8.26GQKK68 pKa = 7.11LTKK71 pKa = 10.11AIRR74 pKa = 11.84GKK76 pKa = 10.59AA77 pKa = 3.19

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

24775

35

3128

266.4

29.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.082 ± 0.311

0.969 ± 0.106

6.155 ± 0.206

6.999 ± 0.425

3.705 ± 0.165

6.692 ± 0.236

1.679 ± 0.187

5.606 ± 0.163

6.39 ± 0.254

8.464 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.999 ± 0.231

4.501 ± 0.425

3.471 ± 0.182

3.726 ± 0.219

5.506 ± 0.268

7.169 ± 0.474

5.97 ± 0.463

6.846 ± 0.194

1.138 ± 0.121

2.934 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski