Proteus phage P16-2532

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SPI1|A0A6B9SPI1_9CAUD Structural protein OS=Proteus phage P16-2532 OX=2692739 PE=4 SV=1
MM1 pKa = 7.87AEE3 pKa = 3.8LAYY6 pKa = 9.35TKK8 pKa = 10.86ASIFQSIVDD17 pKa = 4.18DD18 pKa = 4.38VNTGLSKK25 pKa = 9.96EE26 pKa = 4.39TSLDD30 pKa = 3.31TRR32 pKa = 11.84GDD34 pKa = 3.58FEE36 pKa = 4.22NQLRR40 pKa = 11.84DD41 pKa = 3.57LFEE44 pKa = 5.04EE45 pKa = 4.29NNLMNAPEE53 pKa = 5.22CISVDD58 pKa = 3.34HH59 pKa = 6.9YY60 pKa = 11.41GDD62 pKa = 2.78AWEE65 pKa = 4.69IVQSSEE71 pKa = 3.66FDD73 pKa = 3.62SVEE76 pKa = 4.18LEE78 pKa = 4.23TPLDD82 pKa = 3.83FSGCEE87 pKa = 3.7SSLNCLIIEE96 pKa = 4.25ANEE99 pKa = 4.47LIQASWRR106 pKa = 11.84EE107 pKa = 3.85QVEE110 pKa = 4.23AYY112 pKa = 9.77IEE114 pKa = 4.01EE115 pKa = 4.16LAEE118 pKa = 4.03ALEE121 pKa = 4.34EE122 pKa = 4.18VIEE125 pKa = 4.51GCEE128 pKa = 3.73EE129 pKa = 4.07FGTIDD134 pKa = 3.86EE135 pKa = 4.6LVITDD140 pKa = 4.03GCRR143 pKa = 11.84FGHH146 pKa = 6.32IPHH149 pKa = 6.68LRR151 pKa = 11.84EE152 pKa = 3.31FDD154 pKa = 3.64AGDD157 pKa = 3.68FSVCMWAPNEE167 pKa = 4.12AGEE170 pKa = 4.69SEE172 pKa = 3.88IYY174 pKa = 10.74YY175 pKa = 10.17NFRR178 pKa = 11.84GLEE181 pKa = 3.89LRR183 pKa = 11.84GVVYY187 pKa = 10.63HH188 pKa = 6.78GG189 pKa = 3.79

Molecular weight: 21.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SQ06|A0A6B9SQ06_9CAUD Uncharacterized protein OS=Proteus phage P16-2532 OX=2692739 PE=4 SV=1
MM1 pKa = 7.57TEE3 pKa = 4.01HH4 pKa = 7.09KK5 pKa = 10.45KK6 pKa = 8.96LTRR9 pKa = 11.84VYY11 pKa = 10.39GANLCTFAVEE21 pKa = 4.35RR22 pKa = 11.84GKK24 pKa = 8.44TEE26 pKa = 3.87KK27 pKa = 10.34KK28 pKa = 10.56SPIALRR34 pKa = 11.84PKK36 pKa = 10.14RR37 pKa = 11.84RR38 pKa = 11.84LKK40 pKa = 9.17NTQEE44 pKa = 3.99IFPGILPCANPLYY57 pKa = 10.28RR58 pKa = 11.84MVVIWEE64 pKa = 3.97

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

16105

34

745

187.3

20.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.327 ± 0.353

0.863 ± 0.095

6.631 ± 0.274

7.023 ± 0.27

4.111 ± 0.203

6.992 ± 0.253

1.608 ± 0.114

6.054 ± 0.163

6.234 ± 0.297

7.513 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.8 ± 0.126

4.75 ± 0.19

4.477 ± 0.277

3.26 ± 0.203

5.744 ± 0.172

6.638 ± 0.308

5.917 ± 0.213

7.364 ± 0.329

1.372 ± 0.109

3.322 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski