Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM)
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3545 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3SRC4|A3SRC4_ROSNI C4-dicarboxylate transport transcriptional regulatory protein DctD OS=Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM) OX=89187 GN=ISM_11185 PE=4 SV=1
MM1 pKa = 7.8 PKK3 pKa = 10.3 FIFAYY8 pKa = 10.24 HH9 pKa = 6.75 GGSAPNPDD17 pKa = 4.32 DD18 pKa = 4.0 DD19 pKa = 6.07 AEE21 pKa = 4.11 IARR24 pKa = 11.84 IMGAWQGWLEE34 pKa = 4.1 GMGAACLDD42 pKa = 3.4 MGNPVGLSKK51 pKa = 9.82 TVTADD56 pKa = 3.19 GLRR59 pKa = 11.84 DD60 pKa = 3.85 DD61 pKa = 5.59 GGANPLSGYY70 pKa = 8.86 TIVEE74 pKa = 4.2 AADD77 pKa = 3.27 MAAACDD83 pKa = 3.87 LAQGCPILEE92 pKa = 4.42 GGAGSVEE99 pKa = 3.97 VAPIVEE105 pKa = 4.32 MM106 pKa = 4.93
Molecular weight: 10.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 1.888
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A3SIU1|A3SIU1_ROSNI Metallophos domain-containing protein OS=Roseovarius nubinhibens (strain ATCC BAA-591 / DSM 15170 / ISM) OX=89187 GN=ISM_03245 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3545
0
3545
1098103
18
2140
309.8
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.408 ± 0.056
0.907 ± 0.013
5.963 ± 0.04
6.205 ± 0.04
3.628 ± 0.026
8.906 ± 0.043
2.16 ± 0.023
5.065 ± 0.032
3.066 ± 0.034
10.21 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.845 ± 0.02
2.429 ± 0.021
5.111 ± 0.033
3.202 ± 0.021
6.915 ± 0.042
5.059 ± 0.029
5.234 ± 0.03
7.002 ± 0.034
1.405 ± 0.018
2.281 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here