Cymbidium mosaic virus
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O10690|O10690_9VIRU Movement protein TGBp3 OS=Cymbidium mosaic virus OX=12178 GN=TGB 2 PE=3 SV=1
MM1 pKa = 7.36 EE2 pKa = 5.28 LAYY5 pKa = 10.42 LVRR8 pKa = 11.84 LLEE11 pKa = 4.26 HH12 pKa = 6.58 NKK14 pKa = 9.78 FEE16 pKa = 4.37 RR17 pKa = 11.84 TNLPLSSPLVVHH29 pKa = 7.05 GIAGSGKK36 pKa = 8.17 STILTTFHH44 pKa = 6.21 HH45 pKa = 7.35 HH46 pKa = 5.79 YY47 pKa = 8.85 PAYY50 pKa = 9.7 PIFSHH55 pKa = 6.81 SPTLLDD61 pKa = 3.67 PSNRR65 pKa = 11.84 IYY67 pKa = 9.56 QQCITTDD74 pKa = 3.4 WVPGGAIVDD83 pKa = 4.0 EE84 pKa = 4.58 YY85 pKa = 11.45 NYY87 pKa = 10.79 KK88 pKa = 10.48 ALDD91 pKa = 3.6 YY92 pKa = 10.45 SRR94 pKa = 11.84 CLAVFGDD101 pKa = 4.54 PLQLPHH107 pKa = 6.95 SLQPHH112 pKa = 6.27 YY113 pKa = 10.6 YY114 pKa = 8.96 SSRR117 pKa = 11.84 THH119 pKa = 7.06 RR120 pKa = 11.84 YY121 pKa = 8.48 GPKK124 pKa = 9.2 LTSLLNDD131 pKa = 4.44 LFHH134 pKa = 8.33 LSITSLAPVDD144 pKa = 3.99 SLDD147 pKa = 3.63 YY148 pKa = 10.98 ADD150 pKa = 5.34 PFAVDD155 pKa = 3.51 PSGFTIADD163 pKa = 3.41 EE164 pKa = 4.0 EE165 pKa = 5.15 VYY167 pKa = 11.03 NFVSQQVPGTLLPLDD182 pKa = 4.12 TVGLEE187 pKa = 3.95 YY188 pKa = 10.93 SSVSFYY194 pKa = 11.15 CSDD197 pKa = 3.31 LRR199 pKa = 11.84 SCVVLRR205 pKa = 11.84 PLSSLHH211 pKa = 6.71 RR212 pKa = 11.84 SHH214 pKa = 7.49 PRR216 pKa = 11.84 QGQPHH221 pKa = 7.12 HH222 pKa = 7.04 LRR224 pKa = 11.84 FQCQVV229 pKa = 2.7
Molecular weight: 25.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.688
IPC2_protein 5.817
IPC_protein 5.919
Toseland 6.427
ProMoST 6.338
Dawson 6.262
Bjellqvist 6.237
Wikipedia 6.3
Rodwell 6.262
Grimsley 6.605
Solomon 6.262
Lehninger 6.262
Nozaki 6.547
DTASelect 6.751
Thurlkill 6.781
EMBOSS 6.751
Sillero 6.664
Patrickios 0.998
IPC_peptide 6.287
IPC2_peptide 6.678
IPC2.peptide.svr19 6.538
Protein with the highest isoelectric point:
>tr|Q76SQ1|Q76SQ1_9VIRU Movement protein 2 OS=Cymbidium mosaic virus OX=12178 GN=MP2 PE=4 SV=1
MM1 pKa = 7.74 PGLVPPPDD9 pKa = 3.43 HH10 pKa = 6.93 SKK12 pKa = 11.15 SLFVLAIGITVVSALFVLKK31 pKa = 10.73 SHH33 pKa = 6.93 TFPIAGDD40 pKa = 3.82 NIHH43 pKa = 7.03 RR44 pKa = 11.84 FPSGGQYY51 pKa = 10.64 KK52 pKa = 10.81 DD53 pKa = 3.02 GTKK56 pKa = 10.14 QINYY60 pKa = 8.75 CPPTHH65 pKa = 6.6 ARR67 pKa = 11.84 YY68 pKa = 8.59 PKK70 pKa = 9.87 YY71 pKa = 10.04 PDD73 pKa = 3.92 YY74 pKa = 11.18 KK75 pKa = 10.15 WLAATAAIVIPLCLYY90 pKa = 10.44 ISYY93 pKa = 10.51 HH94 pKa = 6.14 PGNNIRR100 pKa = 11.84 RR101 pKa = 11.84 ICPCCNTYY109 pKa = 10.14 HH110 pKa = 6.8 HH111 pKa = 7.31 PP112 pKa = 3.99
Molecular weight: 12.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.947
IPC2_protein 8.507
IPC_protein 8.492
Toseland 8.551
ProMoST 8.843
Dawson 9.063
Bjellqvist 9.209
Wikipedia 9.136
Rodwell 9.092
Grimsley 8.624
Solomon 9.224
Lehninger 9.194
Nozaki 9.297
DTASelect 9.004
Thurlkill 9.033
EMBOSS 9.194
Sillero 9.297
Patrickios 0.947
IPC_peptide 9.209
IPC2_peptide 8.39
IPC2.peptide.svr19 8.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2072
91
1417
414.4
46.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.784 ± 2.246
1.882 ± 0.413
5.647 ± 0.631
4.778 ± 1.307
4.102 ± 0.141
4.44 ± 0.449
3.571 ± 0.736
6.129 ± 1.021
5.598 ± 1.42
9.99 ± 0.841
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.641 ± 0.492
4.344 ± 0.307
7.432 ± 1.184
3.62 ± 0.568
4.392 ± 0.284
6.419 ± 0.972
6.998 ± 0.53
5.261 ± 0.314
1.11 ± 0.168
3.861 ± 0.66
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here