Paroedura picta
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26691 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A402G689|A0A402G689_9SAUR Plexin_cytopl domain-containing protein (Fragment) OS=Paroedura picta OX=143630 GN=parPi_0031056 PE=4 SV=1
CC1 pKa = 7.16 SLAGSPDD8 pKa = 3.06 VRR10 pKa = 11.84 EE11 pKa = 4.19 WYY13 pKa = 9.96 FAIQSICGFYY23 pKa = 10.08 QFCSSDD29 pKa = 3.36 WEE31 pKa = 4.34 DD32 pKa = 3.43 VSFDD36 pKa = 4.37 LLADD40 pKa = 3.51 QSNDD44 pKa = 2.79 SVRR47 pKa = 11.84 RR48 pKa = 11.84 YY49 pKa = 9.84 IEE51 pKa = 4.07 EE52 pKa = 4.74 CLDD55 pKa = 3.27 SDD57 pKa = 4.67 QNIEE61 pKa = 4.38 KK62 pKa = 9.92 EE63 pKa = 4.18 DD64 pKa = 3.82 SNSRR68 pKa = 11.84 EE69 pKa = 3.9 SMSPIEE75 pKa = 4.02
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.528
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A402F0R4|A0A402F0R4_9SAUR Uncharacterized protein OS=Paroedura picta OX=143630 GN=parPi_0011001 PE=4 SV=1
MM1 pKa = 7.82 KK2 pKa = 10.29 DD3 pKa = 3.36 GPGQAARR10 pKa = 11.84 GALLRR15 pKa = 11.84 GAGRR19 pKa = 11.84 GAAGGSGSAPRR30 pKa = 11.84 LAGVPLHH37 pKa = 6.08 EE38 pKa = 4.87 SRR40 pKa = 11.84 RR41 pKa = 11.84 GPKK44 pKa = 9.72 RR45 pKa = 11.84 SGGGGGGWLRR55 pKa = 11.84 SGQQNLPAWAAPSALRR71 pKa = 11.84 LHH73 pKa = 6.43 TAPGSSRR80 pKa = 11.84 ARR82 pKa = 11.84 RR83 pKa = 11.84 GGGAAGSARR92 pKa = 11.84 VGRR95 pKa = 11.84 APEE98 pKa = 3.83 RR99 pKa = 11.84 TGGCRR104 pKa = 11.84 RR105 pKa = 11.84 APLMPPAGLRR115 pKa = 11.84 GPSAAAPTRR124 pKa = 11.84 PSRR127 pKa = 11.84 AAPRR131 pKa = 11.84 LHH133 pKa = 6.36 GPARR137 pKa = 11.84 EE138 pKa = 3.77 RR139 pKa = 11.84 RR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 QASSLASAAAPLALALARR161 pKa = 11.84 WAPPLLPRR169 pKa = 11.84 ASQSPARR176 pKa = 11.84 SLARR180 pKa = 11.84 SQRR183 pKa = 11.84 TLPRR187 pKa = 11.84 STPRR191 pKa = 11.84 AA192 pKa = 3.5
Molecular weight: 19.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.417
IPC2_protein 10.891
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.144
Rodwell 12.193
Grimsley 12.705
Solomon 13.173
Lehninger 13.071
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 11.915
IPC_peptide 13.173
IPC2_peptide 12.164
IPC2.peptide.svr19 9.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26691
0
26691
11387269
30
32754
426.6
47.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.289 ± 0.018
2.209 ± 0.012
4.813 ± 0.012
7.293 ± 0.02
3.656 ± 0.01
6.562 ± 0.021
2.526 ± 0.008
4.43 ± 0.014
5.938 ± 0.023
9.839 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.007
3.615 ± 0.011
5.923 ± 0.024
4.585 ± 0.015
5.957 ± 0.017
8.066 ± 0.022
5.079 ± 0.014
6.019 ± 0.015
1.29 ± 0.007
2.647 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here