Zhaonella formicivorans
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4397 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R5JBR7|A0A4R5JBR7_9FIRM Putative methylaconitate Delta-isomerase PrpF (Fragment) OS=Zhaonella formicivorans OX=2528593 GN=EYS13_21700 PE=3 SV=1
LL1 pKa = 7.2 NLVEE5 pKa = 5.86 KK6 pKa = 10.29 ICQDD10 pKa = 3.1 SQLIIDD16 pKa = 4.13 IFVNYY21 pKa = 10.1 DD22 pKa = 3.47 CDD24 pKa = 3.49 VDD26 pKa = 3.7 APNIFEE32 pKa = 4.16 RR33 pKa = 5.09
Molecular weight: 3.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.821
IPC_protein 3.643
Toseland 3.452
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.579
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.783
Patrickios 1.888
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A4R5JDJ7|A0A4R5JDJ7_9FIRM Pyridoxal-phosphate dependent enzyme (Fragment) OS=Zhaonella formicivorans OX=2528593 GN=EYS13_18175 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 PFGRR6 pKa = 11.84 PVARR10 pKa = 11.84 QPARR14 pKa = 11.84 APVRR18 pKa = 11.84 PVAVRR23 pKa = 11.84 AQPVRR28 pKa = 11.84 PVVRR32 pKa = 11.84 QPVPRR37 pKa = 11.84 PLPVYY42 pKa = 9.04 PVRR45 pKa = 11.84 PAVRR49 pKa = 11.84 RR50 pKa = 11.84 PAPIAPVVRR59 pKa = 11.84 VAPAPQRR66 pKa = 11.84 RR67 pKa = 3.57
Molecular weight: 7.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.478
IPC2_protein 11.228
IPC_protein 12.661
Toseland 12.808
ProMoST 13.32
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.31
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.32
Sillero 12.808
Patrickios 12.047
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4397
0
4397
1025325
11
2808
233.2
25.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.625 ± 0.042
1.191 ± 0.018
4.481 ± 0.029
7.096 ± 0.047
3.919 ± 0.028
7.645 ± 0.035
1.832 ± 0.017
7.008 ± 0.037
6.515 ± 0.035
10.713 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.449 ± 0.019
3.867 ± 0.027
4.156 ± 0.026
3.648 ± 0.025
4.879 ± 0.037
5.364 ± 0.033
4.958 ± 0.029
7.549 ± 0.038
0.964 ± 0.015
3.14 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here