Gordonia phage Mellie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514A356|A0A514A356_9CAUD Uncharacterized protein OS=Gordonia phage Mellie OX=2591190 GN=25 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.86NPWYY45 pKa = 10.31DD46 pKa = 3.26PTYY49 pKa = 11.17NYY51 pKa = 11.17GLDD54 pKa = 3.95PNGHH58 pKa = 5.7TEE60 pKa = 4.3VTDD63 pKa = 3.08PWKK66 pKa = 10.72RR67 pKa = 11.84HH68 pKa = 3.95TQFPAVLGPMGFEE81 pKa = 4.55GPSIGLPVDD90 pKa = 3.94PPPPPVEE97 pKa = 4.39PEE99 pKa = 3.83PTPDD103 pKa = 3.89IIEE106 pKa = 4.47EE107 pKa = 4.28PTDD110 pKa = 3.47GG111 pKa = 4.67

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514A377|A0A514A377_9CAUD Uncharacterized protein OS=Gordonia phage Mellie OX=2591190 GN=46 PE=4 SV=1
MM1 pKa = 6.43TTNVPTDD8 pKa = 3.46RR9 pKa = 11.84EE10 pKa = 3.99EE11 pKa = 3.81VARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.2TIAEE21 pKa = 4.16LTRR24 pKa = 11.84AGLSAAEE31 pKa = 3.78IAVRR35 pKa = 11.84LNITPRR41 pKa = 11.84TVVRR45 pKa = 11.84QRR47 pKa = 11.84AAAGVSKK54 pKa = 8.9PTYY57 pKa = 9.4PRR59 pKa = 11.84LTEE62 pKa = 4.03EE63 pKa = 4.13QIEE66 pKa = 4.32TARR69 pKa = 11.84RR70 pKa = 11.84LIDD73 pKa = 3.26EE74 pKa = 4.54GAPYY78 pKa = 10.49KK79 pKa = 10.46EE80 pKa = 4.1VARR83 pKa = 11.84TIGCCQSTVAQRR95 pKa = 11.84FPGRR99 pKa = 11.84GWTRR103 pKa = 11.84KK104 pKa = 10.05QIDD107 pKa = 2.96EE108 pKa = 3.93WRR110 pKa = 11.84RR111 pKa = 11.84EE112 pKa = 3.78RR113 pKa = 11.84RR114 pKa = 11.84LWAAASS120 pKa = 3.54

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15603

39

1847

205.3

22.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.44 ± 0.604

0.859 ± 0.141

7.12 ± 0.372

5.582 ± 0.311

2.705 ± 0.149

8.543 ± 0.362

2.166 ± 0.227

4.39 ± 0.153

3.294 ± 0.249

7.672 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.205

2.91 ± 0.181

5.967 ± 0.305

3.275 ± 0.143

7.851 ± 0.503

5.108 ± 0.17

6.749 ± 0.215

7.87 ± 0.267

2.044 ± 0.12

2.307 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski