Kiritimatiellaeota bacterium S-5007
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3062 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P1M4Q9|A0A6P1M4Q9_9BACT Uncharacterized protein OS=Kiritimatiellaeota bacterium S-5007 OX=2697043 GN=GT409_04990 PE=3 SV=1
MM1 pKa = 7.42 TEE3 pKa = 4.18 SKK5 pKa = 10.91 PMVKK9 pKa = 10.02 KK10 pKa = 10.9 GILLSMLLAAICHH23 pKa = 6.3 SDD25 pKa = 3.67 PVDD28 pKa = 4.04 GLDD31 pKa = 3.8 GMAGSSTMDD40 pKa = 4.06 EE41 pKa = 4.08 IEE43 pKa = 4.14 DD44 pKa = 3.68 WFVVTGSVQSVTNGYY59 pKa = 9.15 GAVGMISAAGSNSLWTAEE77 pKa = 4.19 YY78 pKa = 10.57 VSSDD82 pKa = 3.51 VLQTNAVYY90 pKa = 10.68 GLAVRR95 pKa = 11.84 AGNWSSNPGSGTVFAIAIGYY115 pKa = 8.89 YY116 pKa = 9.94 DD117 pKa = 4.25 SEE119 pKa = 4.16 WHH121 pKa = 6.38 EE122 pKa = 4.29 LTNKK126 pKa = 8.89 TFTGGTAGEE135 pKa = 4.12 ITPTINGSYY144 pKa = 7.36 EE145 pKa = 3.99 TLVFTNEE152 pKa = 3.6 LAVFANVAVRR162 pKa = 11.84 VGRR165 pKa = 11.84 TGTSGSWGGFDD176 pKa = 3.54 TVSLTILQSDD186 pKa = 3.71 SDD188 pKa = 4.43 GDD190 pKa = 3.95 GLPDD194 pKa = 3.09 RR195 pKa = 11.84 WEE197 pKa = 3.81 FDD199 pKa = 3.33 YY200 pKa = 11.56 GLNPNDD206 pKa = 3.45 GGTFNFDD213 pKa = 4.58 DD214 pKa = 5.47 GDD216 pKa = 4.49 LGDD219 pKa = 5.24 PDD221 pKa = 5.94 DD222 pKa = 6.79 DD223 pKa = 4.21 GLQNHH228 pKa = 5.73 QEE230 pKa = 4.06 YY231 pKa = 11.02 SLGSDD236 pKa = 3.52 PLVDD240 pKa = 3.32 EE241 pKa = 4.65 WKK243 pKa = 10.61 SRR245 pKa = 11.84 PEE247 pKa = 3.64 KK248 pKa = 10.56 ARR250 pKa = 11.84 LMVINAHH257 pKa = 6.98 PDD259 pKa = 3.08 DD260 pKa = 3.84 EE261 pKa = 6.91 AIFFGGAIPYY271 pKa = 6.95 YY272 pKa = 8.06 TQVRR276 pKa = 11.84 QLPTIVISMTSGLVSEE292 pKa = 4.81 TEE294 pKa = 3.43 IHH296 pKa = 5.38 EE297 pKa = 4.38 AEE299 pKa = 4.31 FRR301 pKa = 11.84 SACWAYY307 pKa = 10.44 GLRR310 pKa = 11.84 NQPVFARR317 pKa = 11.84 FYY319 pKa = 9.31 NNVWNASLDD328 pKa = 4.07 EE329 pKa = 4.46 TWDD332 pKa = 3.15 TWADD336 pKa = 3.55 GVLDD340 pKa = 4.52 GDD342 pKa = 6.08 DD343 pKa = 3.46 ITEE346 pKa = 4.37 GQALAAEE353 pKa = 4.4 TLTYY357 pKa = 9.53 WIRR360 pKa = 11.84 RR361 pKa = 11.84 YY362 pKa = 10.15 QPDD365 pKa = 3.5 VVVTHH370 pKa = 6.83 DD371 pKa = 3.71 LEE373 pKa = 5.77 GEE375 pKa = 4.32 YY376 pKa = 10.98 GHH378 pKa = 6.79 SDD380 pKa = 3.98 HH381 pKa = 7.09 IATAITATNAVQLAADD397 pKa = 3.78 TNYY400 pKa = 10.06 VDD402 pKa = 6.09 GLQPWQVKK410 pKa = 9.82 KK411 pKa = 10.67 LYY413 pKa = 10.89 LHH415 pKa = 6.98 MYY417 pKa = 6.8 PTNQLFHH424 pKa = 7.44 DD425 pKa = 4.91 FWQDD429 pKa = 2.88 VSIDD433 pKa = 3.68 TNADD437 pKa = 2.75 ATADD441 pKa = 3.74 ITPMDD446 pKa = 4.25 AADD449 pKa = 3.61 IGLAFHH455 pKa = 7.22 VYY457 pKa = 10.12 QGTPTNASTVYY468 pKa = 10.48 AVNEE472 pKa = 4.03 TSDD475 pKa = 4.5 AGWEE479 pKa = 4.16 PYY481 pKa = 9.71 PSEE484 pKa = 3.37 WWGLVSSTVGADD496 pKa = 3.03 TATSFTAPDD505 pKa = 3.8 ADD507 pKa = 3.56 NVEE510 pKa = 3.85 ISYY513 pKa = 10.26 VGWARR518 pKa = 11.84 GDD520 pKa = 3.64 FFEE523 pKa = 6.11 HH524 pKa = 5.45 ITVFADD530 pKa = 3.02 SDD532 pKa = 4.21 YY533 pKa = 11.73 DD534 pKa = 3.63 QLPDD538 pKa = 3.54 DD539 pKa = 4.25 WEE541 pKa = 4.3 LAHH544 pKa = 6.8 FPSLEE549 pKa = 4.04 AADD552 pKa = 4.75 PDD554 pKa = 4.48 ADD556 pKa = 4.2 PDD558 pKa = 4.97 GDD560 pKa = 4.08 GLDD563 pKa = 3.68 NAAEE567 pKa = 4.46 FGAGTNPALSDD578 pKa = 3.44 TDD580 pKa = 3.52 GDD582 pKa = 4.22 SFNDD586 pKa = 3.57 GLEE589 pKa = 4.36 VINGGDD595 pKa = 3.91 PLVSDD600 pKa = 4.76 LWRR603 pKa = 11.84 IDD605 pKa = 3.67 YY606 pKa = 10.81 IRR608 pKa = 11.84 DD609 pKa = 3.15 NGADD613 pKa = 3.52 YY614 pKa = 11.42 DD615 pKa = 4.46 LYY617 pKa = 11.4 SSNAVLDD624 pKa = 4.02 LSIGQAAFAVSGGTAWLALQLEE646 pKa = 4.45 KK647 pKa = 11.04 SEE649 pKa = 6.36 DD650 pKa = 3.99 LITWTNAGDD659 pKa = 3.81 TVIWSIPVNTNNAFYY674 pKa = 10.44 RR675 pKa = 11.84 VRR677 pKa = 11.84 SSRR680 pKa = 3.29
Molecular weight: 73.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.923
Patrickios 1.519
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A6P1M7S0|A0A6P1M7S0_9BACT Uncharacterized protein OS=Kiritimatiellaeota bacterium S-5007 OX=2697043 GN=GT409_15365 PE=3 SV=1
MM1 pKa = 7.07 GTIKK5 pKa = 10.15 KK6 pKa = 8.7 WRR8 pKa = 11.84 RR9 pKa = 11.84 TKK11 pKa = 10.12 MSNHH15 pKa = 5.85 KK16 pKa = 9.51 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.42 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ADD24 pKa = 3.32 RR25 pKa = 11.84 HH26 pKa = 5.42 KK27 pKa = 11.04 KK28 pKa = 9.18 KK29 pKa = 10.86
Molecular weight: 3.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.774
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3062
0
3062
1160248
17
2799
378.9
41.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.796 ± 0.044
1.317 ± 0.014
6.008 ± 0.034
6.509 ± 0.045
4.312 ± 0.025
7.92 ± 0.042
2.014 ± 0.019
5.684 ± 0.034
4.701 ± 0.043
9.402 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.503 ± 0.019
3.842 ± 0.028
4.662 ± 0.033
3.473 ± 0.021
5.464 ± 0.039
6.227 ± 0.042
5.507 ± 0.036
7.017 ± 0.036
1.56 ± 0.018
3.084 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here