Penicillium subrubescens
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14038 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q5UHS2|A0A1Q5UHS2_9EURO Uncharacterized protein OS=Penicillium subrubescens OX=1316194 GN=PENSUB_2369 PE=4 SV=1
MM1 pKa = 7.75 SYY3 pKa = 11.48 NNNSDD8 pKa = 3.29 NYY10 pKa = 9.94 NGGNRR15 pKa = 11.84 GSGYY19 pKa = 10.53 DD20 pKa = 3.73 DD21 pKa = 3.22 NHH23 pKa = 7.5 DD24 pKa = 3.95 YY25 pKa = 11.46 GSNQDD30 pKa = 3.84 DD31 pKa = 3.83 YY32 pKa = 12.16 SRR34 pKa = 11.84 GNNSSSEE41 pKa = 4.05 RR42 pKa = 11.84 NQDD45 pKa = 3.05 GGNDD49 pKa = 3.84 GFRR52 pKa = 11.84 GGNDD56 pKa = 3.02 NYY58 pKa = 10.85 SGQSSGFGGQGGNDD72 pKa = 2.98 NYY74 pKa = 11.41 GNQSSGNSGNFGGNQGGSSSGSGLGGLLNQAEE106 pKa = 4.58 GFIKK110 pKa = 10.24 QASASNNNSGNSNNNNNNNNNNNQQDD136 pKa = 3.42 ASVDD140 pKa = 3.5 NFVDD144 pKa = 3.74 SFVDD148 pKa = 3.56 KK149 pKa = 11.1 QVDD152 pKa = 3.61 QYY154 pKa = 12.05 APAGSDD160 pKa = 3.25 SFVNEE165 pKa = 4.2 GVNNMINNAMNKK177 pKa = 9.64 YY178 pKa = 9.92 LL179 pKa = 4.09
Molecular weight: 18.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.579
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.643
Grimsley 3.49
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.656
EMBOSS 3.821
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A1Q5U099|A0A1Q5U099_9EURO Carboxylic ester hydrolase OS=Penicillium subrubescens OX=1316194 GN=PENSUB_6617 PE=3 SV=1
MM1 pKa = 7.29 TRR3 pKa = 11.84 LKK5 pKa = 10.81 GPNARR10 pKa = 11.84 TKK12 pKa = 8.29 TVKK15 pKa = 9.78 TKK17 pKa = 8.07 TTTTRR22 pKa = 11.84 HH23 pKa = 5.39 GPATTTRR30 pKa = 11.84 HH31 pKa = 5.4 GPATTTRR38 pKa = 11.84 HH39 pKa = 5.39 APATHH44 pKa = 6.38 RR45 pKa = 11.84 TTKK48 pKa = 10.26 RR49 pKa = 11.84 RR50 pKa = 11.84 WGRR53 pKa = 11.84 TQPQHH58 pKa = 3.89 HH59 pKa = 7.42 HH60 pKa = 5.69 KK61 pKa = 10.61 RR62 pKa = 11.84 KK63 pKa = 8.35 VTMGDD68 pKa = 3.31 KK69 pKa = 10.93 VSGAMMKK76 pKa = 10.66 LKK78 pKa = 9.56 GTLTGRR84 pKa = 11.84 PGVKK88 pKa = 9.62 VRR90 pKa = 11.84 SS91 pKa = 3.72
Molecular weight: 10.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.515
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.237
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14038
0
14038
5856265
49
6037
417.2
46.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.576 ± 0.017
1.232 ± 0.01
5.646 ± 0.016
6.121 ± 0.023
3.772 ± 0.013
6.859 ± 0.019
2.411 ± 0.009
5.052 ± 0.016
4.601 ± 0.018
8.995 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.225 ± 0.009
3.694 ± 0.011
5.969 ± 0.023
4.076 ± 0.016
6.002 ± 0.016
8.286 ± 0.025
5.98 ± 0.018
6.163 ± 0.015
1.517 ± 0.008
2.824 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here