Hypoxylon sp. CI-4A

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Hypoxylaceae; Hypoxylon; unclassified Hypoxylon

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2VNZ9|A0A1Y2VNZ9_9PEZI Aminotran_1_2 domain-containing protein OS=Hypoxylon sp. CI-4A OX=1001833 GN=M426DRAFT_258948 PE=4 SV=1
MM1 pKa = 6.95KK2 pKa = 8.92TWSSILPFVALLLPALIAADD22 pKa = 3.94CDD24 pKa = 3.96DD25 pKa = 4.16FTFTGQGGSDD35 pKa = 4.0GATEE39 pKa = 5.17PYY41 pKa = 9.76PSSFPVSASINCTTSLASQDD61 pKa = 2.94GGKK64 pKa = 9.76NDD66 pKa = 3.09KK67 pKa = 11.25CEE69 pKa = 3.87FHH71 pKa = 7.34HH72 pKa = 6.88YY73 pKa = 11.2NMGLVVHH80 pKa = 6.23PTINLTTINNSTRR93 pKa = 11.84DD94 pKa = 3.9DD95 pKa = 3.29IFALVKK101 pKa = 10.77DD102 pKa = 4.34KK103 pKa = 11.27ASSAADD109 pKa = 3.26TDD111 pKa = 4.36FNTTIVVNYY120 pKa = 7.41TAHH123 pKa = 5.16TQEE126 pKa = 4.77LSVGQAGHH134 pKa = 6.48VGFTPYY140 pKa = 10.1LRR142 pKa = 11.84CWEE145 pKa = 4.42GVVSDD150 pKa = 5.45CDD152 pKa = 5.59DD153 pKa = 5.71DD154 pKa = 7.4DD155 pKa = 7.46DD156 pKa = 7.5DD157 pKa = 7.47DD158 pKa = 6.2DD159 pKa = 5.92VDD161 pKa = 3.86VDD163 pKa = 3.17EE164 pKa = 4.86DD165 pKa = 4.08TAVRR169 pKa = 11.84VCGLIWLGGTEE180 pKa = 4.2GLSPGLQEE188 pKa = 4.64YY189 pKa = 10.35RR190 pKa = 11.84GEE192 pKa = 4.23EE193 pKa = 3.94NFVSSNVDD201 pKa = 2.59EE202 pKa = 5.05DD203 pKa = 4.87ANGDD207 pKa = 3.86LQPSYY212 pKa = 11.51DD213 pKa = 3.74SVAGQAIVTQGDD225 pKa = 3.24DD226 pKa = 3.15GAAINEE232 pKa = 4.08NHH234 pKa = 6.17EE235 pKa = 4.39VQQ237 pKa = 4.12

Molecular weight: 25.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2W5E6|A0A1Y2W5E6_9PEZI NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit OS=Hypoxylon sp. CI-4A OX=1001833 GN=M426DRAFT_320301 PE=3 SV=1
LL1 pKa = 7.12LHH3 pKa = 7.12LLLFLLFFLLFFLLLLLPLLPLPPLLLSHH32 pKa = 6.97NLPSLLLTRR41 pKa = 11.84PRR43 pKa = 11.84PPMRR47 pKa = 11.84MMQISTNASVRR58 pKa = 11.84GAIAAQPRR66 pKa = 11.84GGVAALQLALANAPAPRR83 pKa = 11.84VGPVVLAPQLDD94 pKa = 3.73VAAFQTQRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84LDD107 pKa = 3.56PDD109 pKa = 3.31QPPLVVVVPRR119 pKa = 11.84RR120 pKa = 11.84LRR122 pKa = 11.84GRR124 pKa = 11.84VLALAVRR131 pKa = 11.84AGAGRR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84VRR144 pKa = 11.84HH145 pKa = 5.6GGWW148 pKa = 2.85

Molecular weight: 16.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11701

0

11701

5364672

50

5457

458.5

50.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.402 ± 0.025

1.174 ± 0.008

5.862 ± 0.015

6.194 ± 0.02

3.753 ± 0.014

6.909 ± 0.023

2.334 ± 0.01

5.065 ± 0.014

4.88 ± 0.022

8.783 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.101 ± 0.008

3.827 ± 0.011

6.041 ± 0.022

3.928 ± 0.015

6.041 ± 0.02

8.227 ± 0.025

6.016 ± 0.015

6.101 ± 0.017

1.494 ± 0.008

2.865 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski