Amino acid dipepetide frequency for Herbaspirillum sp. K1R23-30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.648AlaAla: 14.648 ± 0.136
1.23AlaCys: 1.23 ± 0.026
6.044AlaAsp: 6.044 ± 0.063
6.357AlaGlu: 6.357 ± 0.068
3.948AlaPhe: 3.948 ± 0.055
9.378AlaGly: 9.378 ± 0.091
2.426AlaHis: 2.426 ± 0.04
6.166AlaIle: 6.166 ± 0.059
4.486AlaLys: 4.486 ± 0.057
12.235AlaLeu: 12.235 ± 0.08
3.454AlaMet: 3.454 ± 0.047
3.316AlaAsn: 3.316 ± 0.053
4.838AlaPro: 4.838 ± 0.057
4.658AlaGln: 4.658 ± 0.051
7.142AlaArg: 7.142 ± 0.077
6.953AlaSer: 6.953 ± 0.074
5.799AlaThr: 5.799 ± 0.063
8.095AlaVal: 8.095 ± 0.075
1.428AlaTrp: 1.428 ± 0.028
2.734AlaTyr: 2.734 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.133CysAla: 1.133 ± 0.028
0.139CysCys: 0.139 ± 0.009
0.606CysAsp: 0.606 ± 0.019
0.536CysGlu: 0.536 ± 0.02
0.353CysPhe: 0.353 ± 0.016
1.003CysGly: 1.003 ± 0.027
0.279CysHis: 0.279 ± 0.014
0.5CysIle: 0.5 ± 0.018
0.323CysLys: 0.323 ± 0.014
0.813CysLeu: 0.813 ± 0.021
0.265CysMet: 0.265 ± 0.013
0.302CysAsn: 0.302 ± 0.016
0.465CysPro: 0.465 ± 0.015
0.279CysGln: 0.279 ± 0.013
0.63CysArg: 0.63 ± 0.021
0.598CysSer: 0.598 ± 0.023
0.525CysThr: 0.525 ± 0.023
0.673CysVal: 0.673 ± 0.019
0.105CysTrp: 0.105 ± 0.007
0.253CysTyr: 0.253 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.481AspAla: 6.481 ± 0.073
0.506AspCys: 0.506 ± 0.015
2.81AspAsp: 2.81 ± 0.043
3.198AspGlu: 3.198 ± 0.046
2.127AspPhe: 2.127 ± 0.036
4.407AspGly: 4.407 ± 0.055
1.136AspHis: 1.136 ± 0.023
3.123AspIle: 3.123 ± 0.049
2.167AspLys: 2.167 ± 0.04
5.157AspLeu: 5.157 ± 0.059
1.505AspMet: 1.505 ± 0.03
1.577AspAsn: 1.577 ± 0.028
2.692AspPro: 2.692 ± 0.038
1.671AspGln: 1.671 ± 0.026
3.22AspArg: 3.22 ± 0.037
2.497AspSer: 2.497 ± 0.041
2.807AspThr: 2.807 ± 0.035
4.062AspVal: 4.062 ± 0.053
0.834AspTrp: 0.834 ± 0.021
1.505AspTyr: 1.505 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
6.242GluAla: 6.242 ± 0.064
0.459GluCys: 0.459 ± 0.019
2.387GluAsp: 2.387 ± 0.036
3.232GluGlu: 3.232 ± 0.058
1.974GluPhe: 1.974 ± 0.032
3.526GluGly: 3.526 ± 0.044
1.377GluHis: 1.377 ± 0.029
3.31GluIle: 3.31 ± 0.049
2.679GluLys: 2.679 ± 0.047
5.874GluLeu: 5.874 ± 0.071
1.519GluMet: 1.519 ± 0.027
1.777GluAsn: 1.777 ± 0.033
2.165GluPro: 2.165 ± 0.042
2.891GluGln: 2.891 ± 0.043
4.375GluArg: 4.375 ± 0.052
2.912GluSer: 2.912 ± 0.045
2.762GluThr: 2.762 ± 0.04
3.978GluVal: 3.978 ± 0.055
0.781GluTrp: 0.781 ± 0.023
1.29GluTyr: 1.29 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.134PheAla: 4.134 ± 0.051
0.449PheCys: 0.449 ± 0.015
2.399PheAsp: 2.399 ± 0.037
1.991PheGlu: 1.991 ± 0.03
1.46PhePhe: 1.46 ± 0.03
3.544PheGly: 3.544 ± 0.049
0.797PheHis: 0.797 ± 0.021
1.901PheIle: 1.901 ± 0.032
1.379PheLys: 1.379 ± 0.027
3.196PheLeu: 3.196 ± 0.045
0.898PheMet: 0.898 ± 0.023
1.345PheAsn: 1.345 ± 0.032
1.645PhePro: 1.645 ± 0.033
1.105PheGln: 1.105 ± 0.022
2.038PheArg: 2.038 ± 0.034
2.501PheSer: 2.501 ± 0.034
1.949PheThr: 1.949 ± 0.034
2.653PheVal: 2.653 ± 0.044
0.488PheTrp: 0.488 ± 0.019
1.024PheTyr: 1.024 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
7.953GlyAla: 7.953 ± 0.079
0.864GlyCys: 0.864 ± 0.029
3.766GlyAsp: 3.766 ± 0.045
4.148GlyGlu: 4.148 ± 0.052
3.261GlyPhe: 3.261 ± 0.05
6.178GlyGly: 6.178 ± 0.091
1.788GlyHis: 1.788 ± 0.032
4.775GlyIle: 4.775 ± 0.047
4.134GlyLys: 4.134 ± 0.052
7.67GlyLeu: 7.67 ± 0.073
2.575GlyMet: 2.575 ± 0.041
2.775GlyAsn: 2.775 ± 0.051
2.646GlyPro: 2.646 ± 0.041
2.896GlyGln: 2.896 ± 0.041
4.625GlyArg: 4.625 ± 0.057
4.615GlySer: 4.615 ± 0.052
4.422GlyThr: 4.422 ± 0.06
5.944GlyVal: 5.944 ± 0.069
1.184GlyTrp: 1.184 ± 0.029
2.359GlyTyr: 2.359 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.858HisAla: 2.858 ± 0.043
0.293HisCys: 0.293 ± 0.013
1.25HisAsp: 1.25 ± 0.025
1.198HisGlu: 1.198 ± 0.025
0.967HisPhe: 0.967 ± 0.022
1.982HisGly: 1.982 ± 0.033
0.636HisHis: 0.636 ± 0.024
1.17HisIle: 1.17 ± 0.026
0.668HisLys: 0.668 ± 0.017
2.144HisLeu: 2.144 ± 0.036
0.578HisMet: 0.578 ± 0.017
0.579HisAsn: 0.579 ± 0.018
1.428HisPro: 1.428 ± 0.031
0.791HisGln: 0.791 ± 0.021
1.464HisArg: 1.464 ± 0.03
1.108HisSer: 1.108 ± 0.027
1.086HisThr: 1.086 ± 0.027
1.6HisVal: 1.6 ± 0.03
0.336HisTrp: 0.336 ± 0.015
0.668HisTyr: 0.668 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.166IleAla: 7.166 ± 0.07
0.553IleCys: 0.553 ± 0.018
3.722IleAsp: 3.722 ± 0.043
3.57IleGlu: 3.57 ± 0.045
1.614IlePhe: 1.614 ± 0.035
4.969IleGly: 4.969 ± 0.055
1.06IleHis: 1.06 ± 0.024
2.243IleIle: 2.243 ± 0.038
1.982IleLys: 1.982 ± 0.033
4.284IleLeu: 4.284 ± 0.055
1.054IleMet: 1.054 ± 0.024
1.903IleAsn: 1.903 ± 0.031
2.491IlePro: 2.491 ± 0.039
1.517IleGln: 1.517 ± 0.032
3.13IleArg: 3.13 ± 0.043
3.22IleSer: 3.22 ± 0.046
2.851IleThr: 2.851 ± 0.043
4.231IleVal: 4.231 ± 0.048
0.536IleTrp: 0.536 ± 0.017
1.212IleTyr: 1.212 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
4.376LysAla: 4.376 ± 0.061
0.226LysCys: 0.226 ± 0.011
2.086LysAsp: 2.086 ± 0.04
2.255LysGlu: 2.255 ± 0.041
1.228LysPhe: 1.228 ± 0.027
2.64LysGly: 2.64 ± 0.042
0.868LysHis: 0.868 ± 0.02
2.16LysIle: 2.16 ± 0.037
1.956LysLys: 1.956 ± 0.043
4.34LysLeu: 4.34 ± 0.053
1.113LysMet: 1.113 ± 0.026
1.402LysAsn: 1.402 ± 0.03
2.356LysPro: 2.356 ± 0.04
1.714LysGln: 1.714 ± 0.034
2.618LysArg: 2.618 ± 0.04
2.344LysSer: 2.344 ± 0.038
2.422LysThr: 2.422 ± 0.039
2.893LysVal: 2.893 ± 0.045
0.458LysTrp: 0.458 ± 0.013
0.877LysTyr: 0.877 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
12.261LeuAla: 12.261 ± 0.097
1.017LeuCys: 1.017 ± 0.026
5.416LeuAsp: 5.416 ± 0.061
5.201LeuGlu: 5.201 ± 0.069
3.666LeuPhe: 3.666 ± 0.053
7.608LeuGly: 7.608 ± 0.072
2.397LeuHis: 2.397 ± 0.036
5.001LeuIle: 5.001 ± 0.05
4.106LeuLys: 4.106 ± 0.053
10.244LeuLeu: 10.244 ± 0.103
2.483LeuMet: 2.483 ± 0.041
3.239LeuAsn: 3.239 ± 0.042
5.411LeuPro: 5.411 ± 0.069
3.985LeuGln: 3.985 ± 0.045
6.65LeuArg: 6.65 ± 0.07
6.674LeuSer: 6.674 ± 0.068
5.388LeuThr: 5.388 ± 0.06
7.011LeuVal: 7.011 ± 0.072
1.079LeuTrp: 1.079 ± 0.027
2.276LeuTyr: 2.276 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.906MetAla: 2.906 ± 0.037
0.224MetCys: 0.224 ± 0.01
1.265MetAsp: 1.265 ± 0.03
1.407MetGlu: 1.407 ± 0.028
0.81MetPhe: 0.81 ± 0.022
1.78MetGly: 1.78 ± 0.037
0.637MetHis: 0.637 ± 0.019
1.305MetIle: 1.305 ± 0.03
1.293MetLys: 1.293 ± 0.026
2.978MetLeu: 2.978 ± 0.047
0.738MetMet: 0.738 ± 0.023
1.088MetAsn: 1.088 ± 0.023
1.611MetPro: 1.611 ± 0.026
1.276MetGln: 1.276 ± 0.028
1.898MetArg: 1.898 ± 0.031
1.76MetSer: 1.76 ± 0.032
1.698MetThr: 1.698 ± 0.03
1.817MetVal: 1.817 ± 0.036
0.22MetTrp: 0.22 ± 0.012
0.503MetTyr: 0.503 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.875AsnAla: 3.875 ± 0.046
0.348AsnCys: 0.348 ± 0.013
1.728AsnAsp: 1.728 ± 0.037
1.696AsnGlu: 1.696 ± 0.028
1.151AsnPhe: 1.151 ± 0.027
2.822AsnGly: 2.822 ± 0.055
0.636AsnHis: 0.636 ± 0.019
1.7AsnIle: 1.7 ± 0.033
1.189AsnLys: 1.189 ± 0.025
3.035AsnLeu: 3.035 ± 0.049
0.79AsnMet: 0.79 ± 0.021
1.099AsnAsn: 1.099 ± 0.025
2.037AsnPro: 2.037 ± 0.034
1.093AsnGln: 1.093 ± 0.024
2.024AsnArg: 2.024 ± 0.032
1.587AsnSer: 1.587 ± 0.029
1.728AsnThr: 1.728 ± 0.034
2.346AsnVal: 2.346 ± 0.043
0.45AsnTrp: 0.45 ± 0.015
0.856AsnTyr: 0.856 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
5.69ProAla: 5.69 ± 0.072
0.354ProCys: 0.354 ± 0.015
3.106ProAsp: 3.106 ± 0.043
3.24ProGlu: 3.24 ± 0.046
1.788ProPhe: 1.788 ± 0.033
3.887ProGly: 3.887 ± 0.053
1.098ProHis: 1.098 ± 0.027
2.201ProIle: 2.201 ± 0.039
1.757ProLys: 1.757 ± 0.034
4.607ProLeu: 4.607 ± 0.053
1.175ProMet: 1.175 ± 0.03
1.556ProAsn: 1.556 ± 0.033
2.112ProPro: 2.112 ± 0.043
1.855ProGln: 1.855 ± 0.033
2.463ProArg: 2.463 ± 0.037
2.722ProSer: 2.722 ± 0.04
2.413ProThr: 2.413 ± 0.041
4.023ProVal: 4.023 ± 0.05
0.575ProTrp: 0.575 ± 0.02
1.255ProTyr: 1.255 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.781GlnAla: 4.781 ± 0.059
0.339GlnCys: 0.339 ± 0.015
1.633GlnAsp: 1.633 ± 0.027
1.967GlnGlu: 1.967 ± 0.034
1.4GlnPhe: 1.4 ± 0.022
2.645GlnGly: 2.645 ± 0.038
0.968GlnHis: 0.968 ± 0.024
2.057GlnIle: 2.057 ± 0.036
1.485GlnLys: 1.485 ± 0.031
3.987GlnLeu: 3.987 ± 0.052
1.054GlnMet: 1.054 ± 0.025
1.117GlnAsn: 1.117 ± 0.027
1.879GlnPro: 1.879 ± 0.037
2.023GlnGln: 2.023 ± 0.037
2.846GlnArg: 2.846 ± 0.044
2.195GlnSer: 2.195 ± 0.037
1.974GlnThr: 1.974 ± 0.034
2.764GlnVal: 2.764 ± 0.038
0.557GlnTrp: 0.557 ± 0.016
0.943GlnTyr: 0.943 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
6.321ArgAla: 6.321 ± 0.069
0.574ArgCys: 0.574 ± 0.021
3.399ArgAsp: 3.399 ± 0.047
3.789ArgGlu: 3.789 ± 0.05
2.724ArgPhe: 2.724 ± 0.044
3.895ArgGly: 3.895 ± 0.049
1.733ArgHis: 1.733 ± 0.03
3.88ArgIle: 3.88 ± 0.042
2.604ArgLys: 2.604 ± 0.044
6.962ArgLeu: 6.962 ± 0.072
2.001ArgMet: 2.001 ± 0.033
2.182ArgAsn: 2.182 ± 0.034
2.676ArgPro: 2.676 ± 0.037
2.785ArgGln: 2.785 ± 0.042
4.333ArgArg: 4.333 ± 0.057
3.549ArgSer: 3.549 ± 0.047
3.2ArgThr: 3.2 ± 0.045
4.334ArgVal: 4.334 ± 0.051
0.92ArgTrp: 0.92 ± 0.021
1.932ArgTyr: 1.932 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.421SerAla: 6.421 ± 0.061
0.535SerCys: 0.535 ± 0.02
3.113SerAsp: 3.113 ± 0.045
3.048SerGlu: 3.048 ± 0.042
2.292SerPhe: 2.292 ± 0.039
5.504SerGly: 5.504 ± 0.067
1.3SerHis: 1.3 ± 0.022
3.15SerIle: 3.15 ± 0.037
2.142SerLys: 2.142 ± 0.033
5.979SerLeu: 5.979 ± 0.067
1.608SerMet: 1.608 ± 0.029
1.817SerAsn: 1.817 ± 0.034
2.741SerPro: 2.741 ± 0.04
2.037SerGln: 2.037 ± 0.033
3.618SerArg: 3.618 ± 0.046
3.612SerSer: 3.612 ± 0.055
3.104SerThr: 3.104 ± 0.044
4.252SerVal: 4.252 ± 0.053
0.765SerTrp: 0.765 ± 0.022
1.516SerTyr: 1.516 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
5.897ThrAla: 5.897 ± 0.073
0.445ThrCys: 0.445 ± 0.016
2.73ThrAsp: 2.73 ± 0.042
2.723ThrGlu: 2.723 ± 0.039
1.812ThrPhe: 1.812 ± 0.03
4.674ThrGly: 4.674 ± 0.061
1.186ThrHis: 1.186 ± 0.021
2.79ThrIle: 2.79 ± 0.04
1.647ThrLys: 1.647 ± 0.032
5.887ThrLeu: 5.887 ± 0.067
1.407ThrMet: 1.407 ± 0.029
1.482ThrAsn: 1.482 ± 0.029
3.209ThrPro: 3.209 ± 0.047
1.865ThrGln: 1.865 ± 0.037
3.181ThrArg: 3.181 ± 0.044
3.074ThrSer: 3.074 ± 0.045
3.016ThrThr: 3.016 ± 0.047
4.26ThrVal: 4.26 ± 0.056
0.647ThrTrp: 0.647 ± 0.019
1.344ThrTyr: 1.344 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
8.404ValAla: 8.404 ± 0.072
0.759ValCys: 0.759 ± 0.02
4.05ValAsp: 4.05 ± 0.051
4.115ValGlu: 4.115 ± 0.051
2.704ValPhe: 2.704 ± 0.043
5.143ValGly: 5.143 ± 0.058
1.57ValHis: 1.57 ± 0.031
4.051ValIle: 4.051 ± 0.055
2.891ValLys: 2.891 ± 0.043
7.414ValLeu: 7.414 ± 0.067
2.023ValMet: 2.023 ± 0.032
2.426ValAsn: 2.426 ± 0.042
3.653ValPro: 3.653 ± 0.057
2.527ValGln: 2.527 ± 0.036
4.662ValArg: 4.662 ± 0.048
4.468ValSer: 4.468 ± 0.053
4.14ValThr: 4.14 ± 0.052
5.819ValVal: 5.819 ± 0.075
0.844ValTrp: 0.844 ± 0.023
1.632ValTyr: 1.632 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.029TrpAla: 1.029 ± 0.022
0.156TrpCys: 0.156 ± 0.01
0.594TrpAsp: 0.594 ± 0.019
0.591TrpGlu: 0.591 ± 0.021
0.516TrpPhe: 0.516 ± 0.016
0.811TrpGly: 0.811 ± 0.022
0.373TrpHis: 0.373 ± 0.017
0.72TrpIle: 0.72 ± 0.019
0.556TrpLys: 0.556 ± 0.02
1.614TrpLeu: 1.614 ± 0.031
0.414TrpMet: 0.414 ± 0.014
0.509TrpAsn: 0.509 ± 0.016
0.557TrpPro: 0.557 ± 0.018
0.643TrpGln: 0.643 ± 0.018
0.953TrpArg: 0.953 ± 0.02
0.731TrpSer: 0.731 ± 0.02
0.694TrpThr: 0.694 ± 0.022
0.799TrpVal: 0.799 ± 0.023
0.207TrpTrp: 0.207 ± 0.011
0.325TrpTyr: 0.325 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.757TyrAla: 2.757 ± 0.041
0.309TyrCys: 0.309 ± 0.016
1.379TyrAsp: 1.379 ± 0.034
1.249TyrGlu: 1.249 ± 0.025
1.099TyrPhe: 1.099 ± 0.023
2.184TyrGly: 2.184 ± 0.036
0.54TyrHis: 0.54 ± 0.018
1.104TyrIle: 1.104 ± 0.024
0.889TyrLys: 0.889 ± 0.023
2.691TyrLeu: 2.691 ± 0.043
0.557TyrMet: 0.557 ± 0.019
0.698TyrAsn: 0.698 ± 0.021
1.268TyrPro: 1.268 ± 0.025
1.005TyrGln: 1.005 ± 0.027
1.922TyrArg: 1.922 ± 0.032
1.433TyrSer: 1.433 ± 0.027
1.3TyrThr: 1.3 ± 0.027
1.743TyrVal: 1.743 ± 0.033
0.376TyrTrp: 0.376 ± 0.014
0.748TyrTyr: 0.748 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5690 proteins (1844030 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski