Tick associated circovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus

Average proteome isoelectric point is 8.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6SA29|A0A1W6SA29_9CIRC ATP-dependent helicase Rep OS=Tick associated circovirus 2 OX=2560806 PE=3 SV=1
MM1 pKa = 6.75PAKK4 pKa = 10.22RR5 pKa = 11.84RR6 pKa = 11.84VEE8 pKa = 3.96LRR10 pKa = 11.84EE11 pKa = 3.57AAAARR16 pKa = 11.84WCFTLNNPTDD26 pKa = 3.72EE27 pKa = 4.09EE28 pKa = 4.52VAAVKK33 pKa = 9.94AWNPSEE39 pKa = 3.75YY40 pKa = 9.96HH41 pKa = 4.97YY42 pKa = 10.9AIVGRR47 pKa = 11.84EE48 pKa = 3.88RR49 pKa = 11.84GEE51 pKa = 3.68QGTPHH56 pKa = 6.25LQGFIHH62 pKa = 6.07MKK64 pKa = 9.95KK65 pKa = 10.05KK66 pKa = 10.58GRR68 pKa = 11.84LSALKK73 pKa = 10.18KK74 pKa = 10.21LLSRR78 pKa = 11.84AHH80 pKa = 5.97WEE82 pKa = 3.83KK83 pKa = 11.15ARR85 pKa = 11.84GSDD88 pKa = 3.52EE89 pKa = 5.4DD90 pKa = 3.87NEE92 pKa = 4.64EE93 pKa = 4.29YY94 pKa = 10.39CSKK97 pKa = 10.7EE98 pKa = 3.39GDD100 pKa = 3.71VILTLGIPVKK110 pKa = 10.71GNRR113 pKa = 11.84SDD115 pKa = 4.19LSGAVAAVKK124 pKa = 10.31AGSRR128 pKa = 11.84MADD131 pKa = 2.96IARR134 pKa = 11.84DD135 pKa = 3.68FSEE138 pKa = 5.62VYY140 pKa = 9.99VKK142 pKa = 10.56YY143 pKa = 10.86GRR145 pKa = 11.84GLRR148 pKa = 11.84EE149 pKa = 3.75LALLIGQKK157 pKa = 10.18PRR159 pKa = 11.84DD160 pKa = 4.02FKK162 pKa = 11.2TEE164 pKa = 4.63LIVVTGPSGVGKK176 pKa = 10.12SRR178 pKa = 11.84YY179 pKa = 8.78ANEE182 pKa = 3.98YY183 pKa = 9.94PGTKK187 pKa = 9.25FYY189 pKa = 11.43KK190 pKa = 10.0MKK192 pKa = 10.89GEE194 pKa = 3.66WWDD197 pKa = 4.0GYY199 pKa = 10.42SNEE202 pKa = 4.14EE203 pKa = 4.05VVVIDD208 pKa = 6.03DD209 pKa = 4.39FYY211 pKa = 11.65GWIPFCEE218 pKa = 4.33LLRR221 pKa = 11.84LSDD224 pKa = 4.41RR225 pKa = 11.84YY226 pKa = 8.53PHH228 pKa = 6.36KK229 pKa = 11.21VPVKK233 pKa = 9.5GSYY236 pKa = 10.32VEE238 pKa = 4.71FNSKK242 pKa = 10.6VIIITSNTHH251 pKa = 5.92PEE253 pKa = 3.23TWYY256 pKa = 11.08NEE258 pKa = 4.52EE259 pKa = 4.17KK260 pKa = 10.72CHH262 pKa = 6.21TPALFRR268 pKa = 11.84RR269 pKa = 11.84INRR272 pKa = 11.84WLMWDD277 pKa = 3.02AWQFVDD283 pKa = 5.29APEE286 pKa = 4.24SVKK289 pKa = 10.48KK290 pKa = 10.87YY291 pKa = 9.24PINYY295 pKa = 9.36

Molecular weight: 33.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6SA29|A0A1W6SA29_9CIRC ATP-dependent helicase Rep OS=Tick associated circovirus 2 OX=2560806 PE=3 SV=1
VV1 pKa = 6.75AKK3 pKa = 10.02RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 8.67AAPNRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84WRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84HH19 pKa = 3.61WRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84YY25 pKa = 9.46SNRR28 pKa = 11.84RR29 pKa = 11.84GHH31 pKa = 5.6RR32 pKa = 11.84TNRR35 pKa = 11.84VYY37 pKa = 11.26RR38 pKa = 11.84MRR40 pKa = 11.84FVRR43 pKa = 11.84MYY45 pKa = 9.99RR46 pKa = 11.84QEE48 pKa = 4.15CAGSGDD54 pKa = 3.72VNLKK58 pKa = 10.39FGSDD62 pKa = 3.75GVNIMLDD69 pKa = 3.84DD70 pKa = 4.3FLDD73 pKa = 3.56WGSINWRR80 pKa = 11.84LPFEE84 pKa = 4.93DD85 pKa = 3.68YY86 pKa = 10.67RR87 pKa = 11.84IRR89 pKa = 11.84LAKK92 pKa = 10.46FEE94 pKa = 4.0MRR96 pKa = 11.84PLNEE100 pKa = 4.32NWGSWHH106 pKa = 7.22GIGTNVPIQDD116 pKa = 3.22NHH118 pKa = 8.53LEE120 pKa = 4.18DD121 pKa = 4.32FFKK124 pKa = 10.16KK125 pKa = 9.15TGMTADD131 pKa = 4.67PLANWDD137 pKa = 4.43GARR140 pKa = 11.84QWDD143 pKa = 3.8LRR145 pKa = 11.84KK146 pKa = 10.17GFKK149 pKa = 10.15RR150 pKa = 11.84LFRR153 pKa = 11.84PRR155 pKa = 11.84PQLSITDD162 pKa = 3.61TDD164 pKa = 3.73AGNVTAALWLNNPRR178 pKa = 11.84SAWIPLMKK186 pKa = 10.64KK187 pKa = 10.06NDD189 pKa = 3.67QNLPSPGTRR198 pKa = 11.84VKK200 pKa = 10.59HH201 pKa = 5.33YY202 pKa = 10.42GLGISWPQPHH212 pKa = 7.47DD213 pKa = 4.79AILYY217 pKa = 6.75HH218 pKa = 6.78AKK220 pKa = 8.52VTIYY224 pKa = 11.2VEE226 pKa = 4.29FRR228 pKa = 11.84QMNLTHH234 pKa = 7.05LSSPKK239 pKa = 10.43

Molecular weight: 28.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

534

239

295

267.0

31.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.929 ± 0.673

0.936 ± 0.326

5.431 ± 0.531

5.805 ± 2.071

3.745 ± 0.276

7.116 ± 0.265

2.809 ± 0.338

4.494 ± 0.195

6.742 ± 1.081

7.303 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.428

5.431 ± 1.057

5.431 ± 0.268

2.06 ± 0.546

10.112 ± 2.323

5.243 ± 0.403

3.933 ± 0.158

5.993 ± 1.137

3.933 ± 0.421

4.307 ± 0.603
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski