Amino acid dipepetide frequency for Streptomyces sp. SID8111

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.301AlaAla: 21.301 ± 0.155
1.037AlaCys: 1.037 ± 0.028
8.689AlaAsp: 8.689 ± 0.078
8.905AlaGlu: 8.905 ± 0.09
3.382AlaPhe: 3.382 ± 0.043
13.31AlaGly: 13.31 ± 0.104
2.951AlaHis: 2.951 ± 0.04
3.038AlaIle: 3.038 ± 0.046
2.64AlaLys: 2.64 ± 0.052
14.726AlaLeu: 14.726 ± 0.106
2.489AlaMet: 2.489 ± 0.037
1.75AlaAsn: 1.75 ± 0.035
7.37AlaPro: 7.37 ± 0.077
3.554AlaGln: 3.554 ± 0.045
11.065AlaArg: 11.065 ± 0.09
5.575AlaSer: 5.575 ± 0.06
6.781AlaThr: 6.781 ± 0.062
12.551AlaVal: 12.551 ± 0.107
1.825AlaTrp: 1.825 ± 0.035
2.677AlaTyr: 2.677 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
1.038CysAla: 1.038 ± 0.023
0.092CysCys: 0.092 ± 0.008
0.471CysAsp: 0.471 ± 0.015
0.4CysGlu: 0.4 ± 0.013
0.21CysPhe: 0.21 ± 0.011
0.947CysGly: 0.947 ± 0.02
0.193CysHis: 0.193 ± 0.009
0.16CysIle: 0.16 ± 0.009
0.108CysLys: 0.108 ± 0.006
0.752CysLeu: 0.752 ± 0.019
0.11CysMet: 0.11 ± 0.007
0.123CysAsn: 0.123 ± 0.008
0.493CysPro: 0.493 ± 0.016
0.146CysGln: 0.146 ± 0.009
0.648CysArg: 0.648 ± 0.02
0.413CysSer: 0.413 ± 0.016
0.511CysThr: 0.511 ± 0.016
0.651CysVal: 0.651 ± 0.019
0.125CysTrp: 0.125 ± 0.008
0.148CysTyr: 0.148 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
8.098AspAla: 8.098 ± 0.073
0.415AspCys: 0.415 ± 0.018
4.071AspAsp: 4.071 ± 0.057
4.033AspGlu: 4.033 ± 0.049
1.631AspPhe: 1.631 ± 0.029
6.964AspGly: 6.964 ± 0.07
1.459AspHis: 1.459 ± 0.032
1.843AspIle: 1.843 ± 0.035
1.166AspLys: 1.166 ± 0.027
6.455AspLeu: 6.455 ± 0.056
0.808AspMet: 0.808 ± 0.021
0.963AspAsn: 0.963 ± 0.021
4.758AspPro: 4.758 ± 0.057
1.442AspGln: 1.442 ± 0.028
5.399AspArg: 5.399 ± 0.065
2.356AspSer: 2.356 ± 0.036
3.45AspThr: 3.45 ± 0.041
4.965AspVal: 4.965 ± 0.06
1.033AspTrp: 1.033 ± 0.025
1.089AspTyr: 1.089 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.569GluAla: 7.569 ± 0.084
0.365GluCys: 0.365 ± 0.014
3.043GluAsp: 3.043 ± 0.041
3.915GluGlu: 3.915 ± 0.058
1.398GluPhe: 1.398 ± 0.027
4.45GluGly: 4.45 ± 0.051
1.581GluHis: 1.581 ± 0.033
2.156GluIle: 2.156 ± 0.039
1.52GluLys: 1.52 ± 0.037
6.72GluLeu: 6.72 ± 0.076
0.886GluMet: 0.886 ± 0.024
1.01GluAsn: 1.01 ± 0.029
3.553GluPro: 3.553 ± 0.05
2.33GluGln: 2.33 ± 0.037
5.886GluArg: 5.886 ± 0.062
2.506GluSer: 2.506 ± 0.04
2.907GluThr: 2.907 ± 0.039
4.647GluVal: 4.647 ± 0.055
0.756GluTrp: 0.756 ± 0.021
1.12GluTyr: 1.12 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
3.534PheAla: 3.534 ± 0.046
0.252PheCys: 0.252 ± 0.012
1.931PheAsp: 1.931 ± 0.031
1.446PheGlu: 1.446 ± 0.027
0.842PhePhe: 0.842 ± 0.024
2.924PheGly: 2.924 ± 0.042
0.586PheHis: 0.586 ± 0.017
0.652PheIle: 0.652 ± 0.018
0.48PheLys: 0.48 ± 0.016
2.556PheLeu: 2.556 ± 0.043
0.39PheMet: 0.39 ± 0.014
0.526PheAsn: 0.526 ± 0.016
1.37PhePro: 1.37 ± 0.026
0.621PheGln: 0.621 ± 0.018
1.889PheArg: 1.889 ± 0.034
1.334PheSer: 1.334 ± 0.028
1.913PheThr: 1.913 ± 0.038
2.241PheVal: 2.241 ± 0.034
0.401PheTrp: 0.401 ± 0.014
0.539PheTyr: 0.539 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.041GlyAla: 11.041 ± 0.085
0.828GlyCys: 0.828 ± 0.021
5.509GlyAsp: 5.509 ± 0.06
5.209GlyGlu: 5.209 ± 0.057
2.79GlyPhe: 2.79 ± 0.043
8.971GlyGly: 8.971 ± 0.086
2.4GlyHis: 2.4 ± 0.039
3.333GlyIle: 3.333 ± 0.05
2.297GlyLys: 2.297 ± 0.044
9.529GlyLeu: 9.529 ± 0.093
1.979GlyMet: 1.979 ± 0.035
1.679GlyAsn: 1.679 ± 0.037
5.566GlyPro: 5.566 ± 0.068
2.551GlyGln: 2.551 ± 0.046
8.403GlyArg: 8.403 ± 0.075
5.15GlySer: 5.15 ± 0.058
6.631GlyThr: 6.631 ± 0.063
7.529GlyVal: 7.529 ± 0.06
1.646GlyTrp: 1.646 ± 0.032
2.152GlyTyr: 2.152 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.741HisAla: 2.741 ± 0.039
0.21HisCys: 0.21 ± 0.012
1.463HisAsp: 1.463 ± 0.029
1.216HisGlu: 1.216 ± 0.03
0.627HisPhe: 0.627 ± 0.02
2.475HisGly: 2.475 ± 0.038
0.711HisHis: 0.711 ± 0.023
0.641HisIle: 0.641 ± 0.018
0.335HisLys: 0.335 ± 0.013
2.51HisLeu: 2.51 ± 0.037
0.335HisMet: 0.335 ± 0.012
0.35HisAsn: 0.35 ± 0.015
1.856HisPro: 1.856 ± 0.033
0.607HisGln: 0.607 ± 0.019
2.283HisArg: 2.283 ± 0.04
0.923HisSer: 0.923 ± 0.022
1.368HisThr: 1.368 ± 0.029
1.785HisVal: 1.785 ± 0.029
0.38HisTrp: 0.38 ± 0.014
0.449HisTyr: 0.449 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
4.426IleAla: 4.426 ± 0.056
0.262IleCys: 0.262 ± 0.011
2.114IleAsp: 2.114 ± 0.034
1.873IleGlu: 1.873 ± 0.033
0.635IlePhe: 0.635 ± 0.019
3.38IleGly: 3.38 ± 0.047
0.567IleHis: 0.567 ± 0.018
0.8IleIle: 0.8 ± 0.022
0.69IleLys: 0.69 ± 0.021
2.319IleLeu: 2.319 ± 0.042
0.427IleMet: 0.427 ± 0.016
0.638IleAsn: 0.638 ± 0.021
1.628IlePro: 1.628 ± 0.032
0.65IleGln: 0.65 ± 0.02
2.158IleArg: 2.158 ± 0.03
1.484IleSer: 1.484 ± 0.031
2.084IleThr: 2.084 ± 0.033
2.627IleVal: 2.627 ± 0.045
0.315IleTrp: 0.315 ± 0.013
0.469IleTyr: 0.469 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.794LysAla: 2.794 ± 0.049
0.117LysCys: 0.117 ± 0.008
1.367LysAsp: 1.367 ± 0.034
1.261LysGlu: 1.261 ± 0.032
0.419LysPhe: 0.419 ± 0.017
1.846LysGly: 1.846 ± 0.039
0.413LysHis: 0.413 ± 0.016
0.815LysIle: 0.815 ± 0.024
0.913LysLys: 0.913 ± 0.037
1.899LysLeu: 1.899 ± 0.04
0.366LysMet: 0.366 ± 0.015
0.516LysAsn: 0.516 ± 0.021
1.244LysPro: 1.244 ± 0.026
0.694LysGln: 0.694 ± 0.022
1.461LysArg: 1.461 ± 0.031
1.007LysSer: 1.007 ± 0.024
1.219LysThr: 1.219 ± 0.034
1.799LysVal: 1.799 ± 0.039
0.246LysTrp: 0.246 ± 0.013
0.439LysTyr: 0.439 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
15.007LeuAla: 15.007 ± 0.117
0.853LeuCys: 0.853 ± 0.022
6.929LeuAsp: 6.929 ± 0.062
4.904LeuGlu: 4.904 ± 0.05
2.539LeuPhe: 2.539 ± 0.044
9.202LeuGly: 9.202 ± 0.086
2.312LeuHis: 2.312 ± 0.037
3.098LeuIle: 3.098 ± 0.045
2.044LeuLys: 2.044 ± 0.039
11.517LeuLeu: 11.517 ± 0.103
1.673LeuMet: 1.673 ± 0.034
1.603LeuAsn: 1.603 ± 0.03
6.543LeuPro: 6.543 ± 0.063
2.098LeuGln: 2.098 ± 0.031
9.138LeuArg: 9.138 ± 0.069
5.135LeuSer: 5.135 ± 0.051
7.099LeuThr: 7.099 ± 0.067
8.811LeuVal: 8.811 ± 0.077
1.275LeuTrp: 1.275 ± 0.03
1.87LeuTyr: 1.87 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.221MetAla: 2.221 ± 0.035
0.131MetCys: 0.131 ± 0.009
0.876MetAsp: 0.876 ± 0.021
0.753MetGlu: 0.753 ± 0.021
0.438MetPhe: 0.438 ± 0.015
1.291MetGly: 1.291 ± 0.029
0.354MetHis: 0.354 ± 0.014
0.649MetIle: 0.649 ± 0.021
0.413MetLys: 0.413 ± 0.014
1.632MetLeu: 1.632 ± 0.028
0.282MetMet: 0.282 ± 0.012
0.435MetAsn: 0.435 ± 0.015
1.103MetPro: 1.103 ± 0.022
0.447MetGln: 0.447 ± 0.017
1.434MetArg: 1.434 ± 0.028
1.296MetSer: 1.296 ± 0.029
1.568MetThr: 1.568 ± 0.027
1.259MetVal: 1.259 ± 0.03
0.229MetTrp: 0.229 ± 0.011
0.321MetTyr: 0.321 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.082AsnAla: 2.082 ± 0.04
0.164AsnCys: 0.164 ± 0.009
0.891AsnAsp: 0.891 ± 0.023
0.764AsnGlu: 0.764 ± 0.019
0.439AsnPhe: 0.439 ± 0.017
1.756AsnGly: 1.756 ± 0.034
0.381AsnHis: 0.381 ± 0.014
0.601AsnIle: 0.601 ± 0.019
0.401AsnLys: 0.401 ± 0.018
1.58AsnLeu: 1.58 ± 0.027
0.274AsnMet: 0.274 ± 0.012
0.392AsnAsn: 0.392 ± 0.016
1.305AsnPro: 1.305 ± 0.027
0.465AsnGln: 0.465 ± 0.016
1.258AsnArg: 1.258 ± 0.031
0.826AsnSer: 0.826 ± 0.021
1.023AsnThr: 1.023 ± 0.027
1.353AsnVal: 1.353 ± 0.029
0.263AsnTrp: 0.263 ± 0.013
0.389AsnTyr: 0.389 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
9.064ProAla: 9.064 ± 0.088
0.337ProCys: 0.337 ± 0.015
4.938ProAsp: 4.938 ± 0.053
4.501ProGlu: 4.501 ± 0.053
1.544ProPhe: 1.544 ± 0.029
7.102ProGly: 7.102 ± 0.066
1.495ProHis: 1.495 ± 0.027
1.069ProIle: 1.069 ± 0.024
1.108ProLys: 1.108 ± 0.027
5.37ProLeu: 5.37 ± 0.055
1.017ProMet: 1.017 ± 0.026
0.782ProAsn: 0.782 ± 0.021
3.838ProPro: 3.838 ± 0.059
1.654ProGln: 1.654 ± 0.036
4.238ProArg: 4.238 ± 0.052
3.113ProSer: 3.113 ± 0.046
3.002ProThr: 3.002 ± 0.044
5.826ProVal: 5.826 ± 0.058
0.867ProTrp: 0.867 ± 0.023
1.379ProTyr: 1.379 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.512GlnAla: 3.512 ± 0.044
0.156GlnCys: 0.156 ± 0.01
1.418GlnAsp: 1.418 ± 0.024
1.522GlnGlu: 1.522 ± 0.027
0.624GlnPhe: 0.624 ± 0.017
2.253GlnGly: 2.253 ± 0.043
0.63GlnHis: 0.63 ± 0.016
0.994GlnIle: 0.994 ± 0.024
0.613GlnLys: 0.613 ± 0.022
2.826GlnLeu: 2.826 ± 0.038
0.473GlnMet: 0.473 ± 0.017
0.465GlnAsn: 0.465 ± 0.015
1.574GlnPro: 1.574 ± 0.033
1.241GlnGln: 1.241 ± 0.034
2.321GlnArg: 2.321 ± 0.036
1.155GlnSer: 1.155 ± 0.029
1.239GlnThr: 1.239 ± 0.025
2.309GlnVal: 2.309 ± 0.036
0.433GlnTrp: 0.433 ± 0.017
0.574GlnTyr: 0.574 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
10.697ArgAla: 10.697 ± 0.081
0.601ArgCys: 0.601 ± 0.016
4.741ArgAsp: 4.741 ± 0.056
5.13ArgGlu: 5.13 ± 0.064
2.35ArgPhe: 2.35 ± 0.036
6.006ArgGly: 6.006 ± 0.067
2.286ArgHis: 2.286 ± 0.036
3.261ArgIle: 3.261 ± 0.046
1.646ArgLys: 1.646 ± 0.034
9.349ArgLeu: 9.349 ± 0.086
1.826ArgMet: 1.826 ± 0.03
1.359ArgAsn: 1.359 ± 0.028
5.469ArgPro: 5.469 ± 0.057
2.326ArgGln: 2.326 ± 0.033
8.415ArgArg: 8.415 ± 0.09
4.077ArgSer: 4.077 ± 0.044
5.739ArgThr: 5.739 ± 0.06
6.255ArgVal: 6.255 ± 0.056
1.397ArgTrp: 1.397 ± 0.025
1.811ArgTyr: 1.811 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.319SerAla: 6.319 ± 0.057
0.376SerCys: 0.376 ± 0.015
2.615SerAsp: 2.615 ± 0.041
2.326SerGlu: 2.326 ± 0.036
1.464SerPhe: 1.464 ± 0.029
5.73SerGly: 5.73 ± 0.062
0.941SerHis: 0.941 ± 0.025
1.234SerIle: 1.234 ± 0.029
0.957SerLys: 0.957 ± 0.027
4.644SerLeu: 4.644 ± 0.059
0.981SerMet: 0.981 ± 0.023
0.795SerAsn: 0.795 ± 0.022
3.057SerPro: 3.057 ± 0.041
1.088SerGln: 1.088 ± 0.025
3.649SerArg: 3.649 ± 0.043
2.557SerSer: 2.557 ± 0.047
2.875SerThr: 2.875 ± 0.042
4.152SerVal: 4.152 ± 0.045
0.817SerTrp: 0.817 ± 0.022
1.114SerTyr: 1.114 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
9.019ThrAla: 9.019 ± 0.087
0.445ThrCys: 0.445 ± 0.016
3.764ThrAsp: 3.764 ± 0.049
3.341ThrGlu: 3.341 ± 0.042
1.588ThrPhe: 1.588 ± 0.031
6.899ThrGly: 6.899 ± 0.065
1.229ThrHis: 1.229 ± 0.026
1.496ThrIle: 1.496 ± 0.036
1.067ThrLys: 1.067 ± 0.03
5.726ThrLeu: 5.726 ± 0.056
0.909ThrMet: 0.909 ± 0.021
0.903ThrAsn: 0.903 ± 0.024
4.153ThrPro: 4.153 ± 0.047
1.237ThrGln: 1.237 ± 0.031
4.198ThrArg: 4.198 ± 0.051
2.925ThrSer: 2.925 ± 0.042
3.774ThrThr: 3.774 ± 0.052
6.4ThrVal: 6.4 ± 0.063
0.896ThrTrp: 0.896 ± 0.023
1.307ThrTyr: 1.307 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.782ValAla: 10.782 ± 0.076
0.773ValCys: 0.773 ± 0.02
5.237ValAsp: 5.237 ± 0.051
4.836ValGlu: 4.836 ± 0.058
2.411ValPhe: 2.411 ± 0.039
6.418ValGly: 6.418 ± 0.068
2.048ValHis: 2.048 ± 0.034
2.729ValIle: 2.729 ± 0.043
1.707ValLys: 1.707 ± 0.035
9.736ValLeu: 9.736 ± 0.086
1.405ValMet: 1.405 ± 0.029
1.574ValAsn: 1.574 ± 0.03
5.565ValPro: 5.565 ± 0.056
2.046ValGln: 2.046 ± 0.031
7.697ValArg: 7.697 ± 0.068
4.097ValSer: 4.097 ± 0.049
5.89ValThr: 5.89 ± 0.058
8.386ValVal: 8.386 ± 0.089
1.178ValTrp: 1.178 ± 0.026
1.579ValTyr: 1.579 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.631TrpAla: 1.631 ± 0.026
0.158TrpCys: 0.158 ± 0.008
0.803TrpAsp: 0.803 ± 0.021
0.749TrpGlu: 0.749 ± 0.018
0.485TrpPhe: 0.485 ± 0.016
1.019TrpGly: 1.019 ± 0.024
0.343TrpHis: 0.343 ± 0.015
0.51TrpIle: 0.51 ± 0.017
0.343TrpLys: 0.343 ± 0.015
1.782TrpLeu: 1.782 ± 0.031
0.273TrpMet: 0.273 ± 0.013
0.382TrpAsn: 0.382 ± 0.017
0.78TrpPro: 0.78 ± 0.021
0.607TrpGln: 0.607 ± 0.018
1.343TrpArg: 1.343 ± 0.028
0.869TrpSer: 0.869 ± 0.024
1.033TrpThr: 1.033 ± 0.024
0.942TrpVal: 0.942 ± 0.025
0.309TrpTrp: 0.309 ± 0.012
0.351TrpTyr: 0.351 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.739TyrAla: 2.739 ± 0.041
0.171TyrCys: 0.171 ± 0.01
1.528TyrAsp: 1.528 ± 0.029
1.233TyrGlu: 1.233 ± 0.027
0.611TyrPhe: 0.611 ± 0.02
2.25TyrGly: 2.25 ± 0.037
0.384TyrHis: 0.384 ± 0.015
0.466TyrIle: 0.466 ± 0.016
0.388TyrLys: 0.388 ± 0.017
2.021TyrLeu: 2.021 ± 0.035
0.23TyrMet: 0.23 ± 0.01
0.362TyrAsn: 0.362 ± 0.013
1.016TyrPro: 1.016 ± 0.023
0.529TyrGln: 0.529 ± 0.017
1.86TyrArg: 1.86 ± 0.033
0.894TyrSer: 0.894 ± 0.024
1.169TyrThr: 1.169 ± 0.028
1.605TyrVal: 1.605 ± 0.027
0.319TyrTrp: 0.319 ± 0.014
0.412TyrTyr: 0.412 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6477 proteins (1922263 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski