Amino acid dipepetide frequency for Prevotella sp. CAG:1124

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.725AlaAla: 6.725 ± 0.113	1.182AlaCys: 1.182 ± 0.045	5.485AlaAsp: 5.485 ± 0.074	4.88AlaGlu: 4.88 ± 0.091	3.296AlaPhe: 3.296 ± 0.069	5.638AlaGly: 5.638 ± 0.088	1.237AlaHis: 1.237 ± 0.044	4.538AlaIle: 4.538 ± 0.09	4.179AlaLys: 4.179 ± 0.081	6.859AlaLeu: 6.859 ± 0.109	2.372AlaMet: 2.372 ± 0.056	3.184AlaAsn: 3.184 ± 0.063	2.394AlaPro: 2.394 ± 0.053	2.573AlaGln: 2.573 ± 0.065	3.723AlaArg: 3.723 ± 0.068	4.169AlaSer: 4.169 ± 0.062	4.258AlaThr: 4.258 ± 0.087	5.869AlaVal: 5.869 ± 0.116	0.828AlaTrp: 0.828 ± 0.034	3.067AlaTyr: 3.067 ± 0.067	0.0AlaXaa: 0.0 ± 0.0
Cys	1.065CysAla: 1.065 ± 0.04	0.251CysCys: 0.251 ± 0.02	0.784CysAsp: 0.784 ± 0.029	0.682CysGlu: 0.682 ± 0.029	0.569CysPhe: 0.569 ± 0.028	1.326CysGly: 1.326 ± 0.047	0.335CysHis: 0.335 ± 0.021	0.979CysIle: 0.979 ± 0.034	0.677CysLys: 0.677 ± 0.031	1.157CysLeu: 1.157 ± 0.041	0.39CysMet: 0.39 ± 0.022	0.641CysAsn: 0.641 ± 0.031	0.666CysPro: 0.666 ± 0.036	0.321CysGln: 0.321 ± 0.021	0.866CysArg: 0.866 ± 0.033	0.828CysSer: 0.828 ± 0.031	0.796CysThr: 0.796 ± 0.035	0.983CysVal: 0.983 ± 0.034	0.166CysTrp: 0.166 ± 0.014	0.599CysTyr: 0.599 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.292AspAla: 4.292 ± 0.084	0.839AspCys: 0.839 ± 0.032	3.892AspAsp: 3.892 ± 0.075	4.345AspGlu: 4.345 ± 0.081	3.025AspPhe: 3.025 ± 0.062	5.633AspGly: 5.633 ± 0.117	0.834AspHis: 0.834 ± 0.033	4.606AspIle: 4.606 ± 0.079	4.095AspLys: 4.095 ± 0.077	4.217AspLeu: 4.217 ± 0.082	2.018AspMet: 2.018 ± 0.059	3.532AspAsn: 3.532 ± 0.074	1.894AspPro: 1.894 ± 0.055	1.104AspGln: 1.104 ± 0.043	2.76AspArg: 2.76 ± 0.061	3.22AspSer: 3.22 ± 0.077	3.267AspThr: 3.267 ± 0.068	4.485AspVal: 4.485 ± 0.091	0.826AspTrp: 0.826 ± 0.033	3.014AspTyr: 3.014 ± 0.068	0.001AspXaa: 0.001 ± 0.001
Glu	4.759GluAla: 4.759 ± 0.1	0.776GluCys: 0.776 ± 0.037	3.106GluAsp: 3.106 ± 0.061	3.965GluGlu: 3.965 ± 0.077	2.2GluPhe: 2.2 ± 0.049	4.148GluGly: 4.148 ± 0.078	1.323GluHis: 1.323 ± 0.043	4.074GluIle: 4.074 ± 0.079	3.959GluLys: 3.959 ± 0.091	5.326GluLeu: 5.326 ± 0.081	1.86GluMet: 1.86 ± 0.051	3.152GluAsn: 3.152 ± 0.069	1.893GluPro: 1.893 ± 0.047	2.231GluGln: 2.231 ± 0.061	3.595GluArg: 3.595 ± 0.065	2.836GluSer: 2.836 ± 0.063	3.205GluThr: 3.205 ± 0.069	3.721GluVal: 3.721 ± 0.067	0.834GluTrp: 0.834 ± 0.032	2.701GluTyr: 2.701 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.13PheAla: 3.13 ± 0.056	0.767PheCys: 0.767 ± 0.034	2.861PheAsp: 2.861 ± 0.059	2.119PheGlu: 2.119 ± 0.051	1.92PhePhe: 1.92 ± 0.052	3.364PheGly: 3.364 ± 0.071	0.743PheHis: 0.743 ± 0.032	3.066PheIle: 3.066 ± 0.071	2.243PheLys: 2.243 ± 0.058	3.208PheLeu: 3.208 ± 0.077	1.342PheMet: 1.342 ± 0.043	2.451PheAsn: 2.451 ± 0.065	1.438PhePro: 1.438 ± 0.042	0.89PheGln: 0.89 ± 0.034	2.117PheArg: 2.117 ± 0.059	2.976PheSer: 2.976 ± 0.069	3.078PheThr: 3.078 ± 0.066	2.957PheVal: 2.957 ± 0.057	0.514PheTrp: 0.514 ± 0.029	1.713PheTyr: 1.713 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.819GlyAla: 4.819 ± 0.092	1.149GlyCys: 1.149 ± 0.045	4.22GlyAsp: 4.22 ± 0.07	4.038GlyGlu: 4.038 ± 0.074	3.149GlyPhe: 3.149 ± 0.066	5.548GlyGly: 5.548 ± 0.101	1.509GlyHis: 1.509 ± 0.045	5.142GlyIle: 5.142 ± 0.081	4.867GlyLys: 4.867 ± 0.078	6.136GlyLeu: 6.136 ± 0.101	2.351GlyMet: 2.351 ± 0.058	3.842GlyAsn: 3.842 ± 0.073	1.454GlyPro: 1.454 ± 0.045	2.29GlyGln: 2.29 ± 0.053	4.091GlyArg: 4.091 ± 0.079	4.237GlySer: 4.237 ± 0.077	4.596GlyThr: 4.596 ± 0.1	5.312GlyVal: 5.312 ± 0.093	1.05GlyTrp: 1.05 ± 0.041	3.333GlyTyr: 3.333 ± 0.08	0.001GlyXaa: 0.001 ± 0.001
His	1.271HisAla: 1.271 ± 0.044	0.335HisCys: 0.335 ± 0.024	1.202HisAsp: 1.202 ± 0.044	1.094HisGlu: 1.094 ± 0.041	0.875HisPhe: 0.875 ± 0.032	1.568HisGly: 1.568 ± 0.048	0.465HisHis: 0.465 ± 0.029	1.286HisIle: 1.286 ± 0.035	1.073HisLys: 1.073 ± 0.04	1.52HisLeu: 1.52 ± 0.042	0.337HisMet: 0.337 ± 0.021	0.957HisAsn: 0.957 ± 0.035	0.949HisPro: 0.949 ± 0.036	0.493HisGln: 0.493 ± 0.028	0.946HisArg: 0.946 ± 0.032	1.047HisSer: 1.047 ± 0.038	1.05HisThr: 1.05 ± 0.034	1.249HisVal: 1.249 ± 0.04	0.235HisTrp: 0.235 ± 0.018	0.857HisTyr: 0.857 ± 0.032	0.001HisXaa: 0.001 ± 0.001
Ile	4.974IleAla: 4.974 ± 0.099	0.961IleCys: 0.961 ± 0.037	4.632IleAsp: 4.632 ± 0.076	4.042IleGlu: 4.042 ± 0.079	2.391IlePhe: 2.391 ± 0.059	4.551IleGly: 4.551 ± 0.087	1.133IleHis: 1.133 ± 0.043	4.456IleIle: 4.456 ± 0.096	4.023IleLys: 4.023 ± 0.08	4.924IleLeu: 4.924 ± 0.085	1.745IleMet: 1.745 ± 0.056	3.496IleAsn: 3.496 ± 0.063	2.81IlePro: 2.81 ± 0.064	1.68IleGln: 1.68 ± 0.049	3.305IleArg: 3.305 ± 0.066	4.274IleSer: 4.274 ± 0.074	4.386IleThr: 4.386 ± 0.088	4.471IleVal: 4.471 ± 0.081	0.579IleTrp: 0.579 ± 0.03	2.577IleTyr: 2.577 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.961LysAla: 4.961 ± 0.084	0.615LysCys: 0.615 ± 0.031	3.853LysAsp: 3.853 ± 0.08	4.268LysGlu: 4.268 ± 0.087	2.063LysPhe: 2.063 ± 0.054	4.063LysGly: 4.063 ± 0.08	1.15LysHis: 1.15 ± 0.038	3.868LysIle: 3.868 ± 0.076	4.154LysLys: 4.154 ± 0.083	4.826LysLeu: 4.826 ± 0.081	2.025LysMet: 2.025 ± 0.055	3.033LysAsn: 3.033 ± 0.064	2.197LysPro: 2.197 ± 0.054	2.028LysGln: 2.028 ± 0.057	3.203LysArg: 3.203 ± 0.073	3.272LysSer: 3.272 ± 0.072	3.574LysThr: 3.574 ± 0.074	3.967LysVal: 3.967 ± 0.07	0.738LysTrp: 0.738 ± 0.025	2.827LysTyr: 2.827 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	6.634LeuAla: 6.634 ± 0.096	1.367LeuCys: 1.367 ± 0.042	5.058LeuAsp: 5.058 ± 0.084	4.433LeuGlu: 4.433 ± 0.081	3.856LeuPhe: 3.856 ± 0.076	5.742LeuGly: 5.742 ± 0.1	1.702LeuHis: 1.702 ± 0.049	4.757LeuIle: 4.757 ± 0.082	5.471LeuLys: 5.471 ± 0.083	7.673LeuLeu: 7.673 ± 0.126	2.519LeuMet: 2.519 ± 0.067	4.16LeuAsn: 4.16 ± 0.072	3.863LeuPro: 3.863 ± 0.068	2.59LeuGln: 2.59 ± 0.062	4.731LeuArg: 4.731 ± 0.082	6.01LeuSer: 6.01 ± 0.096	5.167LeuThr: 5.167 ± 0.093	5.33LeuVal: 5.33 ± 0.096	0.922LeuTrp: 0.922 ± 0.035	3.467LeuTyr: 3.467 ± 0.082	0.001LeuXaa: 0.001 ± 0.001
Met	2.741MetAla: 2.741 ± 0.056	0.337MetCys: 0.337 ± 0.022	1.494MetAsp: 1.494 ± 0.045	1.691MetGlu: 1.691 ± 0.046	1.243MetPhe: 1.243 ± 0.042	1.805MetGly: 1.805 ± 0.045	0.535MetHis: 0.535 ± 0.026	1.447MetIle: 1.447 ± 0.043	2.54MetLys: 2.54 ± 0.054	2.926MetLeu: 2.926 ± 0.064	0.922MetMet: 0.922 ± 0.035	1.597MetAsn: 1.597 ± 0.045	1.428MetPro: 1.428 ± 0.04	1.074MetGln: 1.074 ± 0.037	1.686MetArg: 1.686 ± 0.049	1.904MetSer: 1.904 ± 0.055	1.822MetThr: 1.822 ± 0.049	1.722MetVal: 1.722 ± 0.054	0.261MetTrp: 0.261 ± 0.02	0.982MetTyr: 0.982 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.842AsnAla: 3.842 ± 0.068	0.571AsnCys: 0.571 ± 0.03	3.035AsnAsp: 3.035 ± 0.067	2.831AsnGlu: 2.831 ± 0.072	2.009AsnPhe: 2.009 ± 0.051	4.107AsnGly: 4.107 ± 0.08	0.853AsnHis: 0.853 ± 0.032	3.85AsnIle: 3.85 ± 0.076	2.906AsnLys: 2.906 ± 0.069	3.839AsnLeu: 3.839 ± 0.08	1.516AsnMet: 1.516 ± 0.042	2.703AsnAsn: 2.703 ± 0.077	2.252AsnPro: 2.252 ± 0.054	1.26AsnGln: 1.26 ± 0.041	2.389AsnArg: 2.389 ± 0.055	2.702AsnSer: 2.702 ± 0.058	2.836AsnThr: 2.836 ± 0.061	3.705AsnVal: 3.705 ± 0.078	0.574AsnTrp: 0.574 ± 0.028	2.109AsnTyr: 2.109 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	2.81ProAla: 2.81 ± 0.061	0.456ProCys: 0.456 ± 0.024	2.79ProAsp: 2.79 ± 0.05	3.133ProGlu: 3.133 ± 0.057	1.787ProPhe: 1.787 ± 0.047	2.55ProGly: 2.55 ± 0.062	0.689ProHis: 0.689 ± 0.031	2.07ProIle: 2.07 ± 0.054	1.859ProLys: 1.859 ± 0.047	3.133ProLeu: 3.133 ± 0.069	1.073ProMet: 1.073 ± 0.044	1.431ProAsn: 1.431 ± 0.045	0.864ProPro: 0.864 ± 0.038	1.289ProGln: 1.289 ± 0.039	1.453ProArg: 1.453 ± 0.047	2.29ProSer: 2.29 ± 0.056	2.088ProThr: 2.088 ± 0.057	3.147ProVal: 3.147 ± 0.059	0.472ProTrp: 0.472 ± 0.023	1.701ProTyr: 1.701 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	2.437GlnAla: 2.437 ± 0.061	0.415GlnCys: 0.415 ± 0.021	1.589GlnAsp: 1.589 ± 0.048	1.744GlnGlu: 1.744 ± 0.049	1.164GlnPhe: 1.164 ± 0.041	1.879GlnGly: 1.879 ± 0.038	0.572GlnHis: 0.572 ± 0.029	1.921GlnIle: 1.921 ± 0.053	1.722GlnLys: 1.722 ± 0.053	2.815GlnLeu: 2.815 ± 0.065	1.026GlnMet: 1.026 ± 0.033	1.385GlnAsn: 1.385 ± 0.045	1.183GlnPro: 1.183 ± 0.045	1.209GlnGln: 1.209 ± 0.047	1.696GlnArg: 1.696 ± 0.053	1.622GlnSer: 1.622 ± 0.044	1.88GlnThr: 1.88 ± 0.053	1.89GlnVal: 1.89 ± 0.054	0.417GlnTrp: 0.417 ± 0.021	1.35GlnTyr: 1.35 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	3.221ArgAla: 3.221 ± 0.073	0.593ArgCys: 0.593 ± 0.025	2.551ArgAsp: 2.551 ± 0.063	3.01ArgGlu: 3.01 ± 0.059	2.24ArgPhe: 2.24 ± 0.062	2.94ArgGly: 2.94 ± 0.062	1.315ArgHis: 1.315 ± 0.039	3.685ArgIle: 3.685 ± 0.064	3.476ArgLys: 3.476 ± 0.065	5.243ArgLeu: 5.243 ± 0.103	1.884ArgMet: 1.884 ± 0.046	2.66ArgAsn: 2.66 ± 0.067	2.01ArgPro: 2.01 ± 0.052	2.2ArgGln: 2.2 ± 0.052	3.368ArgArg: 3.368 ± 0.085	2.497ArgSer: 2.497 ± 0.053	2.613ArgThr: 2.613 ± 0.055	2.883ArgVal: 2.883 ± 0.068	0.715ArgTrp: 0.715 ± 0.026	2.399ArgTyr: 2.399 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	4.485SerAla: 4.485 ± 0.084	0.862SerCys: 0.862 ± 0.034	3.404SerAsp: 3.404 ± 0.071	3.181SerGlu: 3.181 ± 0.063	2.878SerPhe: 2.878 ± 0.064	4.825SerGly: 4.825 ± 0.085	1.176SerHis: 1.176 ± 0.038	3.78SerIle: 3.78 ± 0.067	3.01SerLys: 3.01 ± 0.07	5.642SerLeu: 5.642 ± 0.095	1.682SerMet: 1.682 ± 0.043	2.466SerAsn: 2.466 ± 0.063	2.259SerPro: 2.259 ± 0.056	1.702SerGln: 1.702 ± 0.053	2.923SerArg: 2.923 ± 0.067	3.678SerSer: 3.678 ± 0.086	3.252SerThr: 3.252 ± 0.067	4.487SerVal: 4.487 ± 0.078	0.766SerTrp: 0.766 ± 0.034	2.646SerTyr: 2.646 ± 0.071	0.001SerXaa: 0.001 ± 0.001
Thr	4.924ThrAla: 4.924 ± 0.099	0.673ThrCys: 0.673 ± 0.032	4.006ThrAsp: 4.006 ± 0.079	3.185ThrGlu: 3.185 ± 0.076	2.783ThrPhe: 2.783 ± 0.059	4.737ThrGly: 4.737 ± 0.09	1.042ThrHis: 1.042 ± 0.041	3.782ThrIle: 3.782 ± 0.077	2.786ThrLys: 2.786 ± 0.063	5.48ThrLeu: 5.48 ± 0.092	1.477ThrMet: 1.477 ± 0.041	2.395ThrAsn: 2.395 ± 0.068	2.715ThrPro: 2.715 ± 0.067	1.556ThrGln: 1.556 ± 0.046	2.399ThrArg: 2.399 ± 0.051	3.441ThrSer: 3.441 ± 0.079	3.372ThrThr: 3.372 ± 0.074	4.84ThrVal: 4.84 ± 0.082	0.725ThrTrp: 0.725 ± 0.029	2.544ThrTyr: 2.544 ± 0.069	0.0ThrXaa: 0.0 ± 0.0
Val	5.316ValAla: 5.316 ± 0.099	1.167ValCys: 1.167 ± 0.04	4.288ValAsp: 4.288 ± 0.073	4.129ValGlu: 4.129 ± 0.074	2.962ValPhe: 2.962 ± 0.07	4.497ValGly: 4.497 ± 0.087	1.105ValHis: 1.105 ± 0.044	4.519ValIle: 4.519 ± 0.076	4.45ValLys: 4.45 ± 0.076	5.799ValLeu: 5.799 ± 0.105	2.004ValMet: 2.004 ± 0.059	3.647ValAsn: 3.647 ± 0.078	2.781ValPro: 2.781 ± 0.059	1.743ValGln: 1.743 ± 0.054	3.424ValArg: 3.424 ± 0.065	4.722ValSer: 4.722 ± 0.088	4.327ValThr: 4.327 ± 0.079	5.061ValVal: 5.061 ± 0.095	0.772ValTrp: 0.772 ± 0.031	2.821ValTyr: 2.821 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.833TrpAla: 0.833 ± 0.034	0.193TrpCys: 0.193 ± 0.015	0.667TrpAsp: 0.667 ± 0.03	0.572TrpGlu: 0.572 ± 0.026	0.526TrpPhe: 0.526 ± 0.025	0.863TrpGly: 0.863 ± 0.036	0.3TrpHis: 0.3 ± 0.022	0.732TrpIle: 0.732 ± 0.031	0.708TrpLys: 0.708 ± 0.03	1.292TrpLeu: 1.292 ± 0.048	0.399TrpMet: 0.399 ± 0.022	0.734TrpAsn: 0.734 ± 0.03	0.312TrpPro: 0.312 ± 0.02	0.525TrpGln: 0.525 ± 0.028	0.676TrpArg: 0.676 ± 0.03	0.693TrpSer: 0.693 ± 0.034	0.73TrpThr: 0.73 ± 0.03	0.623TrpVal: 0.623 ± 0.026	0.207TrpTrp: 0.207 ± 0.022	0.535TrpTyr: 0.535 ± 0.029	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.217TyrAla: 3.217 ± 0.06	0.573TyrCys: 0.573 ± 0.024	2.982TyrAsp: 2.982 ± 0.068	2.32TyrGlu: 2.32 ± 0.055	1.962TyrPhe: 1.962 ± 0.056	3.347TyrGly: 3.347 ± 0.072	0.792TyrHis: 0.792 ± 0.033	2.852TyrIle: 2.852 ± 0.065	2.487TyrLys: 2.487 ± 0.059	3.434TyrLeu: 3.434 ± 0.082	1.213TyrMet: 1.213 ± 0.043	2.425TyrAsn: 2.425 ± 0.063	1.719TyrPro: 1.719 ± 0.047	1.159TyrGln: 1.159 ± 0.034	2.153TyrArg: 2.153 ± 0.055	2.689TyrSer: 2.689 ± 0.066	2.565TyrThr: 2.565 ± 0.072	2.837TyrVal: 2.837 ± 0.06	0.519TyrTrp: 0.519 ± 0.025	2.073TyrTyr: 2.073 ± 0.068	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.002XaaGln: 0.002 ± 0.002	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.014XaaXaa: 0.014 ± 0.005

Statistics based on 2441 proteins (807868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---