Hymenobacter sp. PAMC 26628

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126PA54|A0A126PA54_9BACT Hydrolase OS=Hymenobacter sp. PAMC 26628 OX=1484118 GN=AXW84_11615 PE=4 SV=1
MM1 pKa = 7.42TATLYY6 pKa = 9.93WPQQPPQSVPVYY18 pKa = 9.4GLSFPEE24 pKa = 4.31SAAGFAGVLEE34 pKa = 4.35QVPSLLDD41 pKa = 3.6CAPGLVDD48 pKa = 4.91VLFSNPRR55 pKa = 11.84CIIYY59 pKa = 10.43AVFDD63 pKa = 3.79SEE65 pKa = 5.49GEE67 pKa = 4.24INGTAMDD74 pKa = 3.89VAAAASGVPFDD85 pKa = 5.72RR86 pKa = 11.84DD87 pKa = 3.49DD88 pKa = 3.45EE89 pKa = 4.36DD90 pKa = 6.3AILRR94 pKa = 11.84GPILVVSRR102 pKa = 4.27

Molecular weight: 10.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126PIR6|A0A126PIR6_9BACT Response regulatory domain-containing protein OS=Hymenobacter sp. PAMC 26628 OX=1484118 GN=AXW84_03855 PE=4 SV=1
MM1 pKa = 7.31VKK3 pKa = 10.46FLGGPLMGVSMGLPFGFTLLLTVAGMMTTVFIISGVGRR41 pKa = 11.84VWVRR45 pKa = 11.84HH46 pKa = 4.84KK47 pKa = 10.24QQQRR51 pKa = 11.84RR52 pKa = 11.84LRR54 pKa = 11.84RR55 pKa = 11.84VPIFSSRR62 pKa = 11.84SRR64 pKa = 11.84NIIKK68 pKa = 9.56VFRR71 pKa = 11.84RR72 pKa = 11.84FGMGGIAFLTPVLLSPIGGTVIATLLGVPRR102 pKa = 11.84GRR104 pKa = 11.84ILLHH108 pKa = 5.71MLWSAVFWGSVLTLASVRR126 pKa = 11.84FGHH129 pKa = 7.41LIHH132 pKa = 6.91HH133 pKa = 7.27

Molecular weight: 14.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4319

0

4319

1435981

26

2603

332.5

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.404 ± 0.061

0.696 ± 0.011

4.974 ± 0.023

4.706 ± 0.034

3.999 ± 0.024

8.231 ± 0.04

2.132 ± 0.018

3.843 ± 0.035

3.653 ± 0.032

11.093 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.698 ± 0.015

3.497 ± 0.034

5.617 ± 0.032

4.48 ± 0.026

6.243 ± 0.038

4.814 ± 0.029

5.934 ± 0.035

7.334 ± 0.031

1.242 ± 0.016

3.409 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski