Chaetoceros tenuissimus DNA virus type-II
Average proteome isoelectric point is 7.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B6VL42|A0A0B6VL42_9VIRU Uncharacterized protein OS=Chaetoceros tenuissimus DNA virus type-II OX=1516127 PE=4 SV=1
MM1 pKa = 7.34 VFRR4 pKa = 11.84 TMSDD8 pKa = 2.61 NSFDD12 pKa = 3.54 IQQIICDD19 pKa = 4.11 EE20 pKa = 4.16 ASIDD24 pKa = 3.83 CSITIDD30 pKa = 3.75 TQPVDD35 pKa = 4.25 DD36 pKa = 5.39 LSASTEE42 pKa = 3.83 YY43 pKa = 11.03 RR44 pKa = 11.84 EE45 pKa = 4.1 VSNNTRR51 pKa = 11.84 IGRR54 pKa = 11.84 AILTYY59 pKa = 9.81 FPPTSEE65 pKa = 4.17 SKK67 pKa = 10.28 YY68 pKa = 10.51 LEE70 pKa = 4.19 PEE72 pKa = 4.81 FILGRR77 pKa = 11.84 ANVHH81 pKa = 6.35 RR82 pKa = 11.84 VSNWFSQYY90 pKa = 8.92 EE91 pKa = 4.14 LCPEE95 pKa = 4.18 TEE97 pKa = 4.08 RR98 pKa = 11.84 KK99 pKa = 9.65 HH100 pKa = 5.06 IHH102 pKa = 6.38 AYY104 pKa = 10.62 VEE106 pKa = 4.96 FKK108 pKa = 10.95 HH109 pKa = 5.91 SVRR112 pKa = 11.84 PFFLTLDD119 pKa = 3.9 TLFKK123 pKa = 10.94 SHH125 pKa = 7.69 DD126 pKa = 4.26 LGWDD130 pKa = 3.03 CRR132 pKa = 11.84 TARR135 pKa = 11.84 KK136 pKa = 7.62 TSKK139 pKa = 10.25 DD140 pKa = 3.42 QRR142 pKa = 11.84 QGAINYY148 pKa = 6.03 VHH150 pKa = 7.01 KK151 pKa = 10.9 LEE153 pKa = 4.29 SRR155 pKa = 11.84 MDD157 pKa = 3.56 GFDD160 pKa = 3.61 PYY162 pKa = 10.09 TWPGNEE168 pKa = 4.39 DD169 pKa = 3.46 KK170 pKa = 10.89 DD171 pKa = 4.61 LKK173 pKa = 10.64 FDD175 pKa = 3.85 PSFVRR180 pKa = 11.84 GNRR183 pKa = 11.84 KK184 pKa = 8.87 KK185 pKa = 10.62 DD186 pKa = 3.18 KK187 pKa = 10.83 RR188 pKa = 11.84 KK189 pKa = 10.3 ARR191 pKa = 11.84 DD192 pKa = 3.38 DD193 pKa = 4.5 AIEE196 pKa = 3.9 EE197 pKa = 3.69 QRR199 pKa = 11.84 QYY201 pKa = 11.01 IEE203 pKa = 4.12 SKK205 pKa = 9.72 PRR207 pKa = 11.84 IWTWDD212 pKa = 3.48 QIVHH216 pKa = 6.07 EE217 pKa = 4.58 SEE219 pKa = 4.04 EE220 pKa = 4.54 SKK222 pKa = 11.11 KK223 pKa = 10.96 LLVTCSWGKK232 pKa = 9.75 KK233 pKa = 6.24 YY234 pKa = 10.62 HH235 pKa = 6.38 EE236 pKa = 4.89 GRR238 pKa = 11.84 HH239 pKa = 5.65 AEE241 pKa = 4.14 DD242 pKa = 3.29 KK243 pKa = 11.0 RR244 pKa = 11.84 RR245 pKa = 11.84 DD246 pKa = 3.52 VTDD249 pKa = 3.59 VIIYY253 pKa = 9.63 YY254 pKa = 10.43 GAGGTGKK261 pKa = 7.4 TTMCMEE267 pKa = 4.92 HH268 pKa = 7.44 DD269 pKa = 4.07 STSSLEE275 pKa = 4.1 PRR277 pKa = 11.84 VEE279 pKa = 3.56 RR280 pKa = 11.84 YY281 pKa = 8.52 YY282 pKa = 10.96 RR283 pKa = 11.84 RR284 pKa = 11.84 NPDD287 pKa = 4.5 DD288 pKa = 4.03 GNFWGGGRR296 pKa = 11.84 TAYY299 pKa = 9.76 KK300 pKa = 8.82 GQRR303 pKa = 11.84 VIHH306 pKa = 5.98 FEE308 pKa = 3.94 EE309 pKa = 5.09 FGGQEE314 pKa = 3.6 PFSRR318 pKa = 11.84 LKK320 pKa = 10.11 EE321 pKa = 3.9 VCDD324 pKa = 3.38 IGKK327 pKa = 9.09 PGPSVNIKK335 pKa = 10.38 GAGVDD340 pKa = 4.22 LNHH343 pKa = 6.56 HH344 pKa = 5.34 TVIFSSNIHH353 pKa = 5.46 PAGWYY358 pKa = 8.22 RR359 pKa = 11.84 HH360 pKa = 5.44 LWKK363 pKa = 10.38 DD364 pKa = 3.92 DD365 pKa = 3.65 PKK367 pKa = 10.84 QFHH370 pKa = 6.3 PFWRR374 pKa = 11.84 RR375 pKa = 11.84 VTQVKK380 pKa = 8.48 FFPSHH385 pKa = 6.74 RR386 pKa = 11.84 PDD388 pKa = 3.07 GTLNIPDD395 pKa = 4.93 EE396 pKa = 4.56 DD397 pKa = 4.34 TPPYY401 pKa = 10.44 IIDD404 pKa = 3.56 QTDD407 pKa = 2.76 EE408 pKa = 4.02 WKK410 pKa = 10.77 EE411 pKa = 3.69 FAGDD415 pKa = 3.4 YY416 pKa = 9.62 SKK418 pKa = 11.65 CMDD421 pKa = 4.39 HH422 pKa = 8.2 ADD424 pKa = 3.15 IHH426 pKa = 5.74 WPVRR430 pKa = 11.84 EE431 pKa = 3.92 VAVEE435 pKa = 4.03 GFSASFGHH443 pKa = 5.68 SHH445 pKa = 4.8 QTQRR449 pKa = 11.84 SS450 pKa = 3.49
Molecular weight: 52.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.037
IPC2_protein 5.919
IPC_protein 6.02
Toseland 6.453
ProMoST 6.402
Dawson 6.338
Bjellqvist 6.326
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.634
Solomon 6.338
Lehninger 6.326
Nozaki 6.62
DTASelect 6.81
Thurlkill 6.825
EMBOSS 6.795
Sillero 6.722
Patrickios 4.291
IPC_peptide 6.351
IPC2_peptide 6.678
IPC2.peptide.svr19 6.619
Protein with the highest isoelectric point:
>tr|A0A0B6VL42|A0A0B6VL42_9VIRU Uncharacterized protein OS=Chaetoceros tenuissimus DNA virus type-II OX=1516127 PE=4 SV=1
MM1 pKa = 6.72 VRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 GGKK8 pKa = 6.81 TAGSKK13 pKa = 9.5 RR14 pKa = 11.84 PKK16 pKa = 9.54 MSSKK20 pKa = 10.78 NFGANRR26 pKa = 11.84 KK27 pKa = 9.23 RR28 pKa = 11.84 DD29 pKa = 3.58 FRR31 pKa = 11.84 RR32 pKa = 11.84 PARR35 pKa = 11.84 KK36 pKa = 9.57 SKK38 pKa = 10.47 AKK40 pKa = 9.87 KK41 pKa = 9.05 ARR43 pKa = 11.84 SMAPAKK49 pKa = 8.89 TVRR52 pKa = 11.84 KK53 pKa = 8.32 STTAGAHH60 pKa = 5.57 SKK62 pKa = 10.22 HH63 pKa = 6.23 FSVIGNPFSKK73 pKa = 9.75 ATQQPQIPDD82 pKa = 2.99 GRR84 pKa = 11.84 MLEE87 pKa = 4.2 SLPRR91 pKa = 11.84 RR92 pKa = 11.84 CQLVTEE98 pKa = 4.4 IRR100 pKa = 11.84 NNVTVGSNPTYY111 pKa = 10.63 ILVAPSLGLAFQAYY125 pKa = 9.86 QDD127 pKa = 3.89 TNVPGGLDD135 pKa = 3.21 SSVYY139 pKa = 9.73 GLQNRR144 pKa = 11.84 GCTVRR149 pKa = 11.84 ANLSATSIEE158 pKa = 4.31 NYY160 pKa = 10.47 NDD162 pKa = 3.07 IAKK165 pKa = 9.27 WRR167 pKa = 11.84 IVSQGINLKK176 pKa = 10.42 LNNVEE181 pKa = 4.74 DD182 pKa = 4.89 EE183 pKa = 4.05 NDD185 pKa = 3.08 GWYY188 pKa = 8.29 EE189 pKa = 3.82 ACRR192 pKa = 11.84 FQHH195 pKa = 6.89 DD196 pKa = 3.7 WTPDD200 pKa = 3.54 EE201 pKa = 4.29 LCLRR205 pKa = 11.84 STEE208 pKa = 4.22 NDD210 pKa = 2.89 ASTISQDD217 pKa = 2.91 EE218 pKa = 4.35 DD219 pKa = 3.75 LVMGVISSSFMNGALNTIGNNMVEE243 pKa = 4.01 QRR245 pKa = 11.84 GYY247 pKa = 11.32 EE248 pKa = 3.98 SGLLKK253 pKa = 10.6 NIHH256 pKa = 6.07 KK257 pKa = 10.72 RR258 pKa = 11.84 MFQLHH263 pKa = 6.56 NNTSAIRR270 pKa = 11.84 PKK272 pKa = 9.88 TLQGQFNYY280 pKa = 10.37 GSEE283 pKa = 3.95 ITFSGTEE290 pKa = 3.81 SEE292 pKa = 5.26 ARR294 pKa = 11.84 FTDD297 pKa = 3.87 VPSNRR302 pKa = 11.84 QLVDD306 pKa = 3.89 SLWHH310 pKa = 6.55 NDD312 pKa = 3.33 YY313 pKa = 11.57 DD314 pKa = 4.92 CILIKK319 pKa = 10.49 LYY321 pKa = 10.22 PRR323 pKa = 11.84 EE324 pKa = 4.06 NTGAAGQTGSALIVNAIQNLEE345 pKa = 4.11 LQYY348 pKa = 11.37 SPTSDD353 pKa = 2.86 LSTYY357 pKa = 10.42 HH358 pKa = 6.83 IANKK362 pKa = 8.51 RR363 pKa = 11.84 ARR365 pKa = 11.84 MVEE368 pKa = 3.73 AKK370 pKa = 10.21 LDD372 pKa = 3.76 KK373 pKa = 11.13 KK374 pKa = 11.09 NNTDD378 pKa = 3.22 AAGEE382 pKa = 4.09 PFVPGSSRR390 pKa = 3.14
Molecular weight: 43.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.98
IPC2_protein 8.96
IPC_protein 8.916
Toseland 9.663
ProMoST 9.443
Dawson 9.897
Bjellqvist 9.619
Wikipedia 10.072
Rodwell 10.218
Grimsley 9.97
Solomon 9.926
Lehninger 9.897
Nozaki 9.75
DTASelect 9.575
Thurlkill 9.75
EMBOSS 10.087
Sillero 9.838
Patrickios 5.766
IPC_peptide 9.926
IPC2_peptide 8.273
IPC2.peptide.svr19 8.044
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1226
386
450
408.7
46.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.444 ± 1.045
1.06 ± 0.408
7.341 ± 0.868
5.791 ± 0.581
4.323 ± 0.5
7.586 ± 0.663
3.263 ± 0.646
5.465 ± 0.211
7.993 ± 1.407
5.628 ± 0.728
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.039 ± 0.308
4.812 ± 1.199
4.16 ± 0.625
3.752 ± 0.314
6.607 ± 0.703
7.912 ± 0.463
5.628 ± 0.441
5.546 ± 0.242
1.631 ± 0.441
3.018 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here