Azoarcus sp. CC-YHH848

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4APE3|A0A4S4APE3_9RHOO DmsE family decaheme c-type cytochrome OS=Azoarcus sp. CC-YHH848 OX=2565932 GN=E6O51_13960 PE=4 SV=1
MM1 pKa = 6.98TAPVQPLPCLTWGYY15 pKa = 10.69RR16 pKa = 11.84PNTDD20 pKa = 3.2PTLPGYY26 pKa = 10.08IVDD29 pKa = 4.06GDD31 pKa = 3.99GGTVANVQPGGLALGVLLAMAPEE54 pKa = 4.26ILSDD58 pKa = 3.91LCAVVLALVQQDD70 pKa = 2.82EE71 pKa = 4.67DD72 pKa = 3.54YY73 pKa = 10.95RR74 pKa = 11.84RR75 pKa = 11.84EE76 pKa = 3.99HH77 pKa = 7.0GIDD80 pKa = 3.95LGDD83 pKa = 3.71ITPKK87 pKa = 10.55DD88 pKa = 3.81ALRR91 pKa = 11.84EE92 pKa = 4.36AIGNAVATLAYY103 pKa = 9.75AAEE106 pKa = 4.59AGLPVEE112 pKa = 4.84LPGLAQLEE120 pKa = 4.5DD121 pKa = 3.87TLL123 pKa = 4.88

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4ARU1|A0A4S4ARU1_9RHOO AMP-binding protein OS=Azoarcus sp. CC-YHH848 OX=2565932 GN=E6O51_05905 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4218

0

4218

1398363

32

4641

331.5

35.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.355 ± 0.068

0.927 ± 0.014

5.483 ± 0.031

5.894 ± 0.038

3.47 ± 0.026

8.804 ± 0.047

2.312 ± 0.02

4.308 ± 0.028

2.656 ± 0.033

11.062 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.021

2.451 ± 0.027

5.266 ± 0.033

3.31 ± 0.023

7.819 ± 0.043

4.714 ± 0.032

4.864 ± 0.039

7.514 ± 0.035

1.423 ± 0.017

2.265 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski