Microbacterium phage Margaery

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mementomorivirus; unclassified Mementomorivirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DHN5|A0A514DHN5_9CAUD Uncharacterized protein OS=Microbacterium phage Margaery OX=2591217 GN=70 PE=4 SV=1
MM1 pKa = 7.36TEE3 pKa = 4.24YY4 pKa = 10.29IRR6 pKa = 11.84LAEE9 pKa = 4.36APQYY13 pKa = 10.46SYY15 pKa = 11.61SYY17 pKa = 7.16PTCSACSVDD26 pKa = 3.85LTHH29 pKa = 7.78DD30 pKa = 3.8GDD32 pKa = 3.52EE33 pKa = 4.42WMCPVCGSTWPSDD46 pKa = 3.47ATDD49 pKa = 3.75GDD51 pKa = 3.79TGNLYY56 pKa = 10.2EE57 pKa = 4.23EE58 pKa = 4.69WSGEE62 pKa = 4.26SLGDD66 pKa = 3.94APTLDD71 pKa = 3.75EE72 pKa = 4.89SAASDD77 pKa = 2.91AGYY80 pKa = 10.42AYY82 pKa = 10.07EE83 pKa = 3.87RR84 pKa = 11.84AEE86 pKa = 4.1RR87 pKa = 11.84KK88 pKa = 9.39RR89 pKa = 11.84AFVSWGWCEE98 pKa = 3.55HH99 pKa = 5.36GMPGEE104 pKa = 4.45CAHH107 pKa = 7.03RR108 pKa = 11.84DD109 pKa = 3.96CPGGTARR116 pKa = 11.84RR117 pKa = 11.84AEE119 pKa = 4.38ANPDD123 pKa = 3.13

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DHR0|A0A514DHR0_9CAUD Uncharacterized protein OS=Microbacterium phage Margaery OX=2591217 GN=76 PE=4 SV=1
MM1 pKa = 7.42SLTRR5 pKa = 11.84GRR7 pKa = 11.84IDD9 pKa = 3.42TMTTATKK16 pKa = 9.93TPAASALADD25 pKa = 3.42VEE27 pKa = 4.38AAFTKK32 pKa = 10.3RR33 pKa = 11.84RR34 pKa = 11.84EE35 pKa = 3.85QAKK38 pKa = 10.14RR39 pKa = 11.84LSEE42 pKa = 3.66QDD44 pKa = 3.22RR45 pKa = 11.84YY46 pKa = 10.11IGSVVRR52 pKa = 11.84TARR55 pKa = 11.84AAGCTWQAIADD66 pKa = 4.31RR67 pKa = 11.84AGTSDD72 pKa = 3.49VAVIKK77 pKa = 10.29AARR80 pKa = 11.84RR81 pKa = 11.84PAKK84 pKa = 10.06PKK86 pKa = 9.9EE87 pKa = 3.9

Molecular weight: 9.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

17948

38

948

187.0

20.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.848 ± 0.357

0.903 ± 0.132

6.391 ± 0.346

7.137 ± 0.333

2.162 ± 0.132

8.859 ± 0.287

2.056 ± 0.171

4.892 ± 0.237

2.446 ± 0.186

6.942 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.112

2.518 ± 0.164

6.296 ± 0.256

2.864 ± 0.13

7.466 ± 0.401

5.053 ± 0.225

7.494 ± 0.344

6.992 ± 0.278

2.028 ± 0.158

2.312 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski