Proteus phage Privateer
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 144 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8R3T7|A0A6G8R3T7_9CAUD Uncharacterized protein OS=Proteus phage Privateer OX=2712958 GN=CPT_Privateer_048 PE=4 SV=1
MM1 pKa = 7.72 IIKK4 pKa = 10.11 KK5 pKa = 9.79 EE6 pKa = 3.84 YY7 pKa = 11.2 VMLNSDD13 pKa = 3.88 LSKK16 pKa = 10.16 IGEE19 pKa = 4.34 EE20 pKa = 4.71 IGVGWNEE27 pKa = 4.07 TCDD30 pKa = 3.79 LLSNHH35 pKa = 7.45 GIHH38 pKa = 7.03 GEE40 pKa = 4.27 DD41 pKa = 3.2 GTGYY45 pKa = 10.53 CIIYY49 pKa = 10.22 NDD51 pKa = 3.46 QKK53 pKa = 11.62 YY54 pKa = 10.66 GDD56 pKa = 4.14 KK57 pKa = 10.99 LDD59 pKa = 4.35 LVMNKK64 pKa = 9.67 LFSDD68 pKa = 3.49 NPEE71 pKa = 3.72 AVEE74 pKa = 4.4 IYY76 pKa = 11.4 VMDD79 pKa = 4.76 DD80 pKa = 3.24 FF81 pKa = 7.38
Molecular weight: 9.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.896
IPC2_protein 4.19
IPC_protein 4.101
Toseland 3.91
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.935
Grimsley 3.834
Solomon 4.075
Lehninger 4.024
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.012
Sillero 4.228
Patrickios 1.964
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.115
Protein with the highest isoelectric point:
>tr|A0A6G8R409|A0A6G8R409_9CAUD Uncharacterized protein OS=Proteus phage Privateer OX=2712958 GN=CPT_Privateer_142 PE=4 SV=1
MM1 pKa = 7.27 RR2 pKa = 11.84 QLYY5 pKa = 9.55 LGRR8 pKa = 11.84 MSIKK12 pKa = 10.27 HH13 pKa = 5.94 PIVRR17 pKa = 11.84 YY18 pKa = 8.52 PRR20 pKa = 11.84 WGGWLEE26 pKa = 4.23 SIILHH31 pKa = 6.4 KK32 pKa = 10.4 LNKK35 pKa = 9.26 VISYY39 pKa = 9.45 EE40 pKa = 4.09 KK41 pKa = 9.21 ITVVINNGTLWMM53 pKa = 4.88
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 9.999
IPC_protein 10.526
Toseland 10.54
ProMoST 10.204
Dawson 10.687
Bjellqvist 10.365
Wikipedia 10.877
Rodwell 11.096
Grimsley 10.76
Solomon 10.745
Lehninger 10.716
Nozaki 10.496
DTASelect 10.365
Thurlkill 10.555
EMBOSS 10.921
Sillero 10.599
Patrickios 10.906
IPC_peptide 10.745
IPC2_peptide 9.121
IPC2.peptide.svr19 8.466
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
144
0
144
28573
30
1529
198.4
22.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.393 ± 0.323
1.193 ± 0.122
6.839 ± 0.209
7.185 ± 0.258
4.34 ± 0.214
6.597 ± 0.261
1.792 ± 0.091
6.839 ± 0.231
8.011 ± 0.287
7.878 ± 0.207
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.017 ± 0.118
5.992 ± 0.156
3.381 ± 0.141
3.118 ± 0.181
4.476 ± 0.173
7.182 ± 0.202
5.491 ± 0.184
6.321 ± 0.208
1.13 ± 0.107
3.825 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here