Lactococcus phage ul36
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0ZVS5|A0ZVS5_9CAUD Uncharacterized protein OS=Lactococcus phage ul36 OX=114416 PE=4 SV=1
MM1 pKa = 7.08 SVKK4 pKa = 9.84 VHH6 pKa = 6.15 FSNGEE11 pKa = 4.04 SIVISEE17 pKa = 4.5 EE18 pKa = 3.94 TRR20 pKa = 11.84 ISAWNSLDD28 pKa = 3.64 KK29 pKa = 11.49 DD30 pKa = 3.32 PDD32 pKa = 3.46 GYY34 pKa = 10.6 YY35 pKa = 10.77 AEE37 pKa = 5.18 GVFSGSNIDD46 pKa = 4.29 SPDD49 pKa = 3.38 LGTSYY54 pKa = 10.29 QHH56 pKa = 6.52 IGLMGLFGSTDD67 pKa = 2.74 WFAIGLDD74 pKa = 3.72 FKK76 pKa = 9.31 TTYY79 pKa = 8.25 KK80 pKa = 10.22 TSAIVSLEE88 pKa = 3.88 EE89 pKa = 4.31 TPWW92 pKa = 4.61
Molecular weight: 10.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.281
IPC2_protein 4.317
IPC_protein 4.19
Toseland 4.012
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.024
Grimsley 3.923
Solomon 4.151
Lehninger 4.113
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.05
EMBOSS 4.088
Sillero 4.317
Patrickios 3.427
IPC_peptide 4.151
IPC2_peptide 4.291
IPC2.peptide.svr19 4.235
Protein with the highest isoelectric point:
>tr|Q8LTR6|Q8LTR6_9CAUD Uncharacterized protein OS=Lactococcus phage ul36 OX=114416 PE=4 SV=1
MM1 pKa = 7.34 IANIIILAYY10 pKa = 10.5 VLFLILGMFITPYY23 pKa = 10.66 AIGKK27 pKa = 7.43 PRR29 pKa = 11.84 SPISIGTATFNILFGIAFLISLYY52 pKa = 10.71 FKK54 pKa = 11.04 LINN57 pKa = 3.82
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.094
IPC2_protein 9.882
IPC_protein 9.955
Toseland 9.75
ProMoST 9.648
Dawson 10.072
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.262
Grimsley 10.204
Solomon 10.16
Lehninger 10.116
Nozaki 9.692
DTASelect 9.823
Thurlkill 9.867
EMBOSS 10.175
Sillero 9.97
Patrickios 7.395
IPC_peptide 10.145
IPC2_peptide 8.624
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
11161
50
908
183.0
20.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.263 ± 0.368
0.753 ± 0.151
6.344 ± 0.235
7.042 ± 0.488
4.614 ± 0.191
6.487 ± 0.451
1.192 ± 0.135
6.908 ± 0.303
8.933 ± 0.505
7.687 ± 0.304
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.67 ± 0.135
6.128 ± 0.255
2.867 ± 0.184
4.426 ± 0.237
3.288 ± 0.214
6.559 ± 0.303
6.137 ± 0.323
6.245 ± 0.242
1.478 ± 0.125
3.978 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here