Canis familiaris papillomavirus 9
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G4XF64|G4XF64_9PAPI Replication protein E1 OS=Canis familiaris papillomavirus 9 OX=1087108 GN=E1 PE=3 SV=1
MM1 pKa = 7.39 IGKK4 pKa = 9.27 DD5 pKa = 2.95 ATLRR9 pKa = 11.84 DD10 pKa = 3.81 IVLEE14 pKa = 4.06 EE15 pKa = 4.37 QPTPLDD21 pKa = 3.72 NLWCDD26 pKa = 3.59 EE27 pKa = 4.17 EE28 pKa = 4.86 LAPSDD33 pKa = 4.41 DD34 pKa = 4.44 EE35 pKa = 5.18 EE36 pKa = 4.86 EE37 pKa = 4.08 EE38 pKa = 4.46 SPPNQNLKK46 pKa = 10.05 PFRR49 pKa = 11.84 IQTYY53 pKa = 9.85 CGTCGRR59 pKa = 11.84 GIRR62 pKa = 11.84 IVVLCTQDD70 pKa = 4.41 GIHH73 pKa = 6.26 SLEE76 pKa = 4.16 SLLCRR81 pKa = 11.84 NTSLCCPTCAATYY94 pKa = 9.87 RR95 pKa = 11.84 FEE97 pKa = 5.84 HH98 pKa = 6.64 GGG100 pKa = 3.37
Molecular weight: 11.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.198
IPC2_protein 4.482
IPC_protein 4.355
Toseland 4.202
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.177
Rodwell 4.19
Grimsley 4.113
Solomon 4.291
Lehninger 4.253
Nozaki 4.418
DTASelect 4.558
Thurlkill 4.215
EMBOSS 4.19
Sillero 4.469
Patrickios 1.036
IPC_peptide 4.304
IPC2_peptide 4.457
IPC2.peptide.svr19 4.381
Protein with the highest isoelectric point:
>tr|G4XF67|G4XF67_9PAPI Minor capsid protein L2 OS=Canis familiaris papillomavirus 9 OX=1087108 GN=L2 PE=3 SV=1
MM1 pKa = 7.4 NLFAPPNLLPAQHH14 pKa = 6.71 RR15 pKa = 11.84 PNTLVQKK22 pKa = 10.66 LWDD25 pKa = 4.55 LLTGPNGWPEE35 pKa = 3.9 EE36 pKa = 4.23 VEE38 pKa = 3.92 LRR40 pKa = 11.84 EE41 pKa = 3.9 LGEE44 pKa = 3.94 RR45 pKa = 11.84 GRR47 pKa = 11.84 GRR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 SPCACARR58 pKa = 11.84 PPTPQTPPHH67 pKa = 6.09 TPPTRR72 pKa = 11.84 SRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 HH77 pKa = 5.44 RR78 pKa = 11.84 SRR80 pKa = 11.84 YY81 pKa = 7.5 TPPQSDD87 pKa = 3.52 STDD90 pKa = 3.29 WDD92 pKa = 3.76 DD93 pKa = 4.38 LQALGACSLTPPLGSPLLTPEE114 pKa = 4.32 EE115 pKa = 4.27 VWFPRR120 pKa = 11.84 SQNSPPVLSPCPGASPLPRR139 pKa = 11.84 PSPGPCTPRR148 pKa = 11.84 PRR150 pKa = 11.84 LLDD153 pKa = 3.79 LDD155 pKa = 3.5 QDD157 pKa = 3.55 QRR159 pKa = 11.84 LPHH162 pKa = 6.87 PSPLPLPDD170 pKa = 3.86 SRR172 pKa = 11.84 PSLTQQRR179 pKa = 11.84 KK180 pKa = 9.11 ALDD183 pKa = 3.23 KK184 pKa = 10.99 ALRR187 pKa = 11.84 QVQKK191 pKa = 10.96 DD192 pKa = 3.54 AFQLEE197 pKa = 4.28 VDD199 pKa = 3.79 IGADD203 pKa = 3.29 LGRR206 pKa = 11.84 FFGKK210 pKa = 10.45 LGIAPRR216 pKa = 11.84 QQ217 pKa = 3.32
Molecular weight: 24.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.197
IPC2_protein 8.873
IPC_protein 9.077
Toseland 9.94
ProMoST 9.575
Dawson 10.072
Bjellqvist 9.838
Wikipedia 10.204
Rodwell 10.233
Grimsley 10.072
Solomon 10.175
Lehninger 10.175
Nozaki 10.145
DTASelect 9.75
Thurlkill 9.984
EMBOSS 10.335
Sillero 10.072
Patrickios 10.058
IPC_peptide 10.189
IPC2_peptide 9.209
IPC2.peptide.svr19 7.686
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2582
100
621
368.9
40.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.468 ± 1.039
2.169 ± 0.601
6.003 ± 0.532
5.693 ± 0.515
3.757 ± 0.496
7.901 ± 0.828
2.091 ± 0.129
3.447 ± 0.382
3.796 ± 0.839
8.869 ± 0.591
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.433 ± 0.226
3.215 ± 0.379
7.978 ± 1.524
4.338 ± 0.482
7.32 ± 0.583
7.397 ± 0.569
6.894 ± 0.247
6.894 ± 1.023
1.549 ± 0.297
2.789 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here